Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains

Abstract To identify the species of butyrolactol-producing Streptomyces strain TP-A0882, whole genome-sequencing of three type strains in a close taxonomic relationship was performed. In silico DNA-DNA hybridization using the genome sequences suggested that Streptomyces sp. TP-A0882 is classified as...

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Autores principales:	Hisayuki Komaki, Kenta Sakurai, Akira Hosoyama, Akane Kimura, Yasuhiro Igarashi, Tomohiko Tamura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24d4efeca1694d4585cb1c25fe106cab
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spelling	oai:doaj.org-article:24d4efeca1694d4585cb1c25fe106cab2021-12-02T12:32:22ZDiversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains10.1038/s41598-018-24921-y2045-2322https://doaj.org/article/24d4efeca1694d4585cb1c25fe106cab2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24921-yhttps://doaj.org/toc/2045-2322Abstract To identify the species of butyrolactol-producing Streptomyces strain TP-A0882, whole genome-sequencing of three type strains in a close taxonomic relationship was performed. In silico DNA-DNA hybridization using the genome sequences suggested that Streptomyces sp. TP-A0882 is classified as Streptomyces diastaticus subsp. ardesiacus. Strain TP-A0882, S. diastaticus subsp. ardesiacus NBRC 15402T, Streptomyces coelicoflavus NBRC 15399T, and Streptomyces rubrogriseus NBRC 15455T harbor at least 14, 14, 10, and 12 biosynthetic gene clusters (BGCs), respectively, coding for nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). All 14 gene clusters were shared by S. diastaticus subsp. ardesiacus strains TP-A0882 and NBRC 15402T, while only four gene clusters were shared by the three distinct species. Although BGCs for bacteriocin, ectoine, indole, melanine, siderophores such as deferrioxamine, terpenes such as albaflavenone, hopene, carotenoid and geosmin are shared by the three species, many BGCs for secondary metabolites such as butyrolactone, lantipeptides, oligosaccharide, some terpenes are species-specific. These results indicate the possibility that strains belonging to the same species possess the same set of secondary metabolite-biosynthetic pathways, whereas strains belonging to distinct species have species-specific pathways, in addition to some common pathways, even if the strains are taxonomically close.Hisayuki KomakiKenta SakuraiAkira HosoyamaAkane KimuraYasuhiro IgarashiTomohiko TamuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hisayuki Komaki Kenta Sakurai Akira Hosoyama Akane Kimura Yasuhiro Igarashi Tomohiko Tamura Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains
description	Abstract To identify the species of butyrolactol-producing Streptomyces strain TP-A0882, whole genome-sequencing of three type strains in a close taxonomic relationship was performed. In silico DNA-DNA hybridization using the genome sequences suggested that Streptomyces sp. TP-A0882 is classified as Streptomyces diastaticus subsp. ardesiacus. Strain TP-A0882, S. diastaticus subsp. ardesiacus NBRC 15402T, Streptomyces coelicoflavus NBRC 15399T, and Streptomyces rubrogriseus NBRC 15455T harbor at least 14, 14, 10, and 12 biosynthetic gene clusters (BGCs), respectively, coding for nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). All 14 gene clusters were shared by S. diastaticus subsp. ardesiacus strains TP-A0882 and NBRC 15402T, while only four gene clusters were shared by the three distinct species. Although BGCs for bacteriocin, ectoine, indole, melanine, siderophores such as deferrioxamine, terpenes such as albaflavenone, hopene, carotenoid and geosmin are shared by the three species, many BGCs for secondary metabolites such as butyrolactone, lantipeptides, oligosaccharide, some terpenes are species-specific. These results indicate the possibility that strains belonging to the same species possess the same set of secondary metabolite-biosynthetic pathways, whereas strains belonging to distinct species have species-specific pathways, in addition to some common pathways, even if the strains are taxonomically close.
format	article
author	Hisayuki Komaki Kenta Sakurai Akira Hosoyama Akane Kimura Yasuhiro Igarashi Tomohiko Tamura
author_facet	Hisayuki Komaki Kenta Sakurai Akira Hosoyama Akane Kimura Yasuhiro Igarashi Tomohiko Tamura
author_sort	Hisayuki Komaki
title	Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains
title_short	Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains
title_full	Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains
title_fullStr	Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains
title_full_unstemmed	Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains
title_sort	diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close streptomyces strains
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/24d4efeca1694d4585cb1c25fe106cab
work_keys_str_mv	AT hisayukikomaki diversityofnonribosomalpeptidesynthetaseandpolyketidesynthasegeneclustersamongtaxonomicallyclosestreptomycesstrains AT kentasakurai diversityofnonribosomalpeptidesynthetaseandpolyketidesynthasegeneclustersamongtaxonomicallyclosestreptomycesstrains AT akirahosoyama diversityofnonribosomalpeptidesynthetaseandpolyketidesynthasegeneclustersamongtaxonomicallyclosestreptomycesstrains AT akanekimura diversityofnonribosomalpeptidesynthetaseandpolyketidesynthasegeneclustersamongtaxonomicallyclosestreptomycesstrains AT yasuhiroigarashi diversityofnonribosomalpeptidesynthetaseandpolyketidesynthasegeneclustersamongtaxonomicallyclosestreptomycesstrains AT tomohikotamura diversityofnonribosomalpeptidesynthetaseandpolyketidesynthasegeneclustersamongtaxonomicallyclosestreptomycesstrains
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Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains

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