Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids

Protein content in the inferior grains (IG) has a great impact on eating quality of indica-japonica rice hybrids. However, the mechanisms of the difference in protein accumulation between IG and superior grains (SG) remains unclear. A field experiment was carried out using an indica-japonica hybrid...

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Autores principales:	Xincheng Zhang, Guoping Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Indica-japonica rice hybrid Protein accumulation Inferior grain High nitrogen Transcriptome Metabolome Botany QK1-989
Acceso en línea:	https://doaj.org/article/24d50c32aa9847158d0a90d9cf2479ee
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spelling	oai:doaj.org-article:24d50c32aa9847158d0a90d9cf2479ee2021-12-02T05:01:42ZTranscriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids2214-662810.1016/j.cpb.2021.100226https://doaj.org/article/24d50c32aa9847158d0a90d9cf2479ee2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2214662821000311https://doaj.org/toc/2214-6628Protein content in the inferior grains (IG) has a great impact on eating quality of indica-japonica rice hybrids. However, the mechanisms of the difference in protein accumulation between IG and superior grains (SG) remains unclear. A field experiment was carried out using an indica-japonica hybrid Yongyou17 and two nitrogen levels. The result showed that prolamin concentration was significantly higher in IG than SG, while there was no significant difference in glutelin concentration between the two types of grains. Totally, 465 metabolites were identified, and 26 “core metabolites” differentially accumulated in IG could be divided into three types. Asparagine and 1-aminocyclopropanecarboxylic acid were markedly increased in IG, while flavonoid compounds were obviously reduced, in comparison with those in SG. Weighted gene co-expression network analysis indicated that bisque4 and darkorange2 modules for type 1 and type 3 of the “core metabolites” were closely associated with the synthesis of ribosomal protein and stress response, respectively. Eight hub genes, i.e. rpL32_8.1, novel.907, RPS15a and RPL13a.4 in bisque4 and OsSAP5, OsHSP20, PRX12 and DUF3741 were identified in darkorange2. In addition, genes WDR, R2R3-MYB and bHLHs were significantly down-regulated in IG, companied by reduced synthesis of flavonoid compounds. IG had higher ethylene and N levels, resulting more protein accumulation. The current study provides a new insight into the regulation network of protein synthesis in IG of indica-japonica rice hybrids.Xincheng ZhangGuoping ZhangElsevierarticleIndica-japonica rice hybridProtein accumulationInferior grainHigh nitrogenTranscriptomeMetabolomeBotanyQK1-989ENCurrent Plant Biology, Vol 28, Iss , Pp 100226- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Indica-japonica rice hybrid Protein accumulation Inferior grain High nitrogen Transcriptome Metabolome Botany QK1-989
spellingShingle	Indica-japonica rice hybrid Protein accumulation Inferior grain High nitrogen Transcriptome Metabolome Botany QK1-989 Xincheng Zhang Guoping Zhang Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
description	Protein content in the inferior grains (IG) has a great impact on eating quality of indica-japonica rice hybrids. However, the mechanisms of the difference in protein accumulation between IG and superior grains (SG) remains unclear. A field experiment was carried out using an indica-japonica hybrid Yongyou17 and two nitrogen levels. The result showed that prolamin concentration was significantly higher in IG than SG, while there was no significant difference in glutelin concentration between the two types of grains. Totally, 465 metabolites were identified, and 26 “core metabolites” differentially accumulated in IG could be divided into three types. Asparagine and 1-aminocyclopropanecarboxylic acid were markedly increased in IG, while flavonoid compounds were obviously reduced, in comparison with those in SG. Weighted gene co-expression network analysis indicated that bisque4 and darkorange2 modules for type 1 and type 3 of the “core metabolites” were closely associated with the synthesis of ribosomal protein and stress response, respectively. Eight hub genes, i.e. rpL32_8.1, novel.907, RPS15a and RPL13a.4 in bisque4 and OsSAP5, OsHSP20, PRX12 and DUF3741 were identified in darkorange2. In addition, genes WDR, R2R3-MYB and bHLHs were significantly down-regulated in IG, companied by reduced synthesis of flavonoid compounds. IG had higher ethylene and N levels, resulting more protein accumulation. The current study provides a new insight into the regulation network of protein synthesis in IG of indica-japonica rice hybrids.
format	article
author	Xincheng Zhang Guoping Zhang
author_facet	Xincheng Zhang Guoping Zhang
author_sort	Xincheng Zhang
title	Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
title_short	Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
title_full	Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
title_fullStr	Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
title_full_unstemmed	Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
title_sort	transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/24d50c32aa9847158d0a90d9cf2479ee
work_keys_str_mv	AT xinchengzhang transcriptomeandmetabolomeprofilingrevealtheregulatorymechanismofproteinaccumulationininferiorgrainsofindicajaponicaricehybrids AT guopingzhang transcriptomeandmetabolomeprofilingrevealtheregulatorymechanismofproteinaccumulationininferiorgrainsofindicajaponicaricehybrids
_version_	1718400758009823232

Transcriptome and metabolome profiling reveal the regulatory mechanism of protein accumulation in inferior grains of indica-japonica rice hybrids

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