Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines

The goal of this study is to investigate the influence of different types of modifiers, such as sodium hyaluronate (NaH), graphene oxide (GO), silica oxycarbide (SiOC) and oxidation process (ox) on physicochemical, morphological, and biological properties of electrospun carbon nanofibers (eCNFs). Sc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wojciech Smolka, Monika Ptas, Agnieszka Panek, Malgorzata Krok-Borkowicz, Marcel Zambrzycki, Maciej Gubernat, Jaroslaw Markowski, Aneta Fraczek-Szczypta
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrospun carbon nanofibers (eCNFs) surface modification microstructure physicochemical properties biocompatibility osteoblast and chondrocytes cell lines Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/24d585990360429e82aa40706ccfd6df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:24d585990360429e82aa40706ccfd6df
record_format	dspace
spelling	oai:doaj.org-article:24d585990360429e82aa40706ccfd6df2021-11-11T17:56:40ZSurface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines10.3390/ma142163701996-1944https://doaj.org/article/24d585990360429e82aa40706ccfd6df2021-10-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/21/6370https://doaj.org/toc/1996-1944The goal of this study is to investigate the influence of different types of modifiers, such as sodium hyaluronate (NaH), graphene oxide (GO), silica oxycarbide (SiOC) and oxidation process (ox) on physicochemical, morphological, and biological properties of electrospun carbon nanofibers (eCNFs). Scanning electron microscopy, X-ray photoelectron spectroscopy and infrared spectroscopy (FTIR) were used to evaluate the microstructure and chemistry of as-prepared and modified CNFs. The electrical properties of CNFs scaffolds were examined using a four-point probe method to evaluate the influence of modifiers on the volume conductivity and surface resistivity of the obtained samples. The wettability of the surfaces of modified and unmodified CNFs scaffolds was also tested by contact angle measurement. During the in vitro study all samples were put into direct contact with human chondrocyte CHON-001 cells and human osteosarcoma MG-63 cells. Their viability was analysed after 72 h in culture. Moreover, the cell morphology and cell area in contact with CNFs was observed by means of fluorescence microscopy. The obtained results show great potential for the modification of CNFs with polymer, ceramic and carbon modifiers, which do not change the fiber form of the substrate but significantly affect their surface and volume properties. Preliminary biological studies have shown that the type of modification of CNFs affects either the rate of increase in the number of cells or the degree of spreading in relation to the unmodified sample. More hydrophilic and low electrically conductive samples such as CNF_ox and CNF_NaH significantly increase cell proliferation, while other GO and SiOC modified samples have an effect on cell adhesion and thus cell spreading. From the point of view of further research and the possibility of combining the electrical properties of modified CNF scaffolds with electrical stimulation, where these scaffolds would be able to transport electrical signals to cells and thus affect cell adhesion, spreading, and consequently tissue regeneration, samples CNF_GO and CNF_SiOC would be the most desirable.Wojciech SmolkaMonika PtasAgnieszka PanekMalgorzata Krok-BorkowiczMarcel ZambrzyckiMaciej GubernatJaroslaw MarkowskiAneta Fraczek-SzczyptaMDPI AGarticleelectrospun carbon nanofibers (eCNFs)surface modificationmicrostructurephysicochemical propertiesbiocompatibilityosteoblast and chondrocytes cell linesTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6370, p 6370 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrospun carbon nanofibers (eCNFs) surface modification microstructure physicochemical properties biocompatibility osteoblast and chondrocytes cell lines Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	electrospun carbon nanofibers (eCNFs) surface modification microstructure physicochemical properties biocompatibility osteoblast and chondrocytes cell lines Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Wojciech Smolka Monika Ptas Agnieszka Panek Malgorzata Krok-Borkowicz Marcel Zambrzycki Maciej Gubernat Jaroslaw Markowski Aneta Fraczek-Szczypta Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines
description	The goal of this study is to investigate the influence of different types of modifiers, such as sodium hyaluronate (NaH), graphene oxide (GO), silica oxycarbide (SiOC) and oxidation process (ox) on physicochemical, morphological, and biological properties of electrospun carbon nanofibers (eCNFs). Scanning electron microscopy, X-ray photoelectron spectroscopy and infrared spectroscopy (FTIR) were used to evaluate the microstructure and chemistry of as-prepared and modified CNFs. The electrical properties of CNFs scaffolds were examined using a four-point probe method to evaluate the influence of modifiers on the volume conductivity and surface resistivity of the obtained samples. The wettability of the surfaces of modified and unmodified CNFs scaffolds was also tested by contact angle measurement. During the in vitro study all samples were put into direct contact with human chondrocyte CHON-001 cells and human osteosarcoma MG-63 cells. Their viability was analysed after 72 h in culture. Moreover, the cell morphology and cell area in contact with CNFs was observed by means of fluorescence microscopy. The obtained results show great potential for the modification of CNFs with polymer, ceramic and carbon modifiers, which do not change the fiber form of the substrate but significantly affect their surface and volume properties. Preliminary biological studies have shown that the type of modification of CNFs affects either the rate of increase in the number of cells or the degree of spreading in relation to the unmodified sample. More hydrophilic and low electrically conductive samples such as CNF_ox and CNF_NaH significantly increase cell proliferation, while other GO and SiOC modified samples have an effect on cell adhesion and thus cell spreading. From the point of view of further research and the possibility of combining the electrical properties of modified CNF scaffolds with electrical stimulation, where these scaffolds would be able to transport electrical signals to cells and thus affect cell adhesion, spreading, and consequently tissue regeneration, samples CNF_GO and CNF_SiOC would be the most desirable.
format	article
author	Wojciech Smolka Monika Ptas Agnieszka Panek Malgorzata Krok-Borkowicz Marcel Zambrzycki Maciej Gubernat Jaroslaw Markowski Aneta Fraczek-Szczypta
author_facet	Wojciech Smolka Monika Ptas Agnieszka Panek Malgorzata Krok-Borkowicz Marcel Zambrzycki Maciej Gubernat Jaroslaw Markowski Aneta Fraczek-Szczypta
author_sort	Wojciech Smolka
title	Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines
title_short	Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines
title_full	Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines
title_fullStr	Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines
title_full_unstemmed	Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines
title_sort	surface modification of carbon nanofibers to improve their biocompatibility in contact with osteoblast and chondrocytes cell lines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24d585990360429e82aa40706ccfd6df
work_keys_str_mv	AT wojciechsmolka surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT monikaptas surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT agnieszkapanek surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT malgorzatakrokborkowicz surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT marcelzambrzycki surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT maciejgubernat surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT jaroslawmarkowski surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines AT anetafraczekszczypta surfacemodificationofcarbonnanofiberstoimprovetheirbiocompatibilityincontactwithosteoblastandchondrocytescelllines
_version_	1718431930932789248

Surface Modification of Carbon Nanofibers to Improve Their Biocompatibility in Contact with Osteoblast and Chondrocytes Cell Lines

Ejemplares similares