Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
Genus <i>Littorina</i> subgenus <i>Neritrema</i> (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (<i>Littorina obtusata</i> and <i>L. fabalis</i>). The anatomy of the adult reproductive system (pallial oviduct) is t...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/24d5c8f0fe5a4172b93b52dca0876ebc |
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| Sumario: | Genus <i>Littorina</i> subgenus <i>Neritrema</i> (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (<i>Littorina obtusata</i> and <i>L. fabalis</i>). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of <i>L. obtusata</i> and <i>L. fabalis</i> by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between <i>L. obtusata</i> and <i>L. fabalis</i>, (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the <i>Littorina</i> species divergence, which has not been reported previously. |
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