Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata

Genus Littorina subgenus Neritrema (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (Littorina obtusata and L. fabalis). The anatomy of the adult reproductive system (pallial oviduct) is t...

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Auteurs principaux:	Arseniy A. Lobov, Irina Y. Babkina, Lavrentii G. Danilov, Alexey E. Masharskiy, Alexander V. Predeus, Natalia A. Mikhailova, Andrei I. Granovitch, Arina L. Maltseva
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	<i>Littorina</i> mollusca 2D DIGE RNA-seq sibling species invertebrate reproduction Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/24d5c8f0fe5a4172b93b52dca0876ebc
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spelling	oai:doaj.org-article:24d5c8f0fe5a4172b93b52dca0876ebc2021-11-25T16:46:53ZSpecies-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>10.3390/biology101110872079-7737https://doaj.org/article/24d5c8f0fe5a4172b93b52dca0876ebc2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1087https://doaj.org/toc/2079-7737Genus <i>Littorina</i> subgenus <i>Neritrema</i> (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (<i>Littorina obtusata</i> and <i>L. fabalis</i>). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of <i>L. obtusata</i> and <i>L. fabalis</i> by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between <i>L. obtusata</i> and <i>L. fabalis</i>, (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the <i>Littorina</i> species divergence, which has not been reported previously.Arseniy A. LobovIrina Y. BabkinaLavrentii G. DanilovAlexey E. MasharskiyAlexander V. PredeusNatalia A. MikhailovaAndrei I. GranovitchArina L. MaltsevaMDPI AGarticle<i>Littorina</i>mollusca2D DIGERNA-seqsibling speciesinvertebrate reproductionBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1087, p 1087 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Littorina</i> mollusca 2D DIGE RNA-seq sibling species invertebrate reproduction Biology (General) QH301-705.5
spellingShingle	<i>Littorina</i> mollusca 2D DIGE RNA-seq sibling species invertebrate reproduction Biology (General) QH301-705.5 Arseniy A. Lobov Irina Y. Babkina Lavrentii G. Danilov Alexey E. Masharskiy Alexander V. Predeus Natalia A. Mikhailova Andrei I. Granovitch Arina L. Maltseva Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
description	Genus <i>Littorina</i> subgenus <i>Neritrema</i> (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (<i>Littorina obtusata</i> and <i>L. fabalis</i>). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of <i>L. obtusata</i> and <i>L. fabalis</i> by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between <i>L. obtusata</i> and <i>L. fabalis</i>, (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the <i>Littorina</i> species divergence, which has not been reported previously.
format	article
author	Arseniy A. Lobov Irina Y. Babkina Lavrentii G. Danilov Alexey E. Masharskiy Alexander V. Predeus Natalia A. Mikhailova Andrei I. Granovitch Arina L. Maltseva
author_facet	Arseniy A. Lobov Irina Y. Babkina Lavrentii G. Danilov Alexey E. Masharskiy Alexander V. Predeus Natalia A. Mikhailova Andrei I. Granovitch Arina L. Maltseva
author_sort	Arseniy A. Lobov
title	Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
title_short	Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
title_full	Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
title_fullStr	Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
title_full_unstemmed	Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>
title_sort	species-specific proteins in the oviducts of snail sibling species: proteotranscriptomic study of <i>littorina fabalis</i> and <i>l. obtusata</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24d5c8f0fe5a4172b93b52dca0876ebc
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Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of <i>Littorina fabalis</i> and <i>L. obtusata</i>

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