Biochemical Mapping of the Inflamed Human Dental Pulp

Dental pulp inflammation, caused by the evolution of caries, involves numerous interrelated activities at a cellular and molecular level. Cytokines, proteases, growth factors, and other biomarkers of the host response may take part in dental pulp’s immune defense. The aim of this pilot study was to...

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Autores principales:	Konstantina Kritikou, Marina Imre, Mihaela Tanase, Arina Vinereanu, Alexandra Ripszky Totan, Tudor-Claudiu Spinu, Radu Ilinca, Daniela Miricescu, Iulia-Ioana Stanescu-Spinu, Maria Greabu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dental pulp inflammation biomarkers enzyme-linked immunosorbent assay oxidative stress extracellular matrix degradation Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/24d93aa20fff436290be673a3c5a23b3
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spelling	oai:doaj.org-article:24d93aa20fff436290be673a3c5a23b32021-11-11T15:23:58ZBiochemical Mapping of the Inflamed Human Dental Pulp10.3390/app1121103952076-3417https://doaj.org/article/24d93aa20fff436290be673a3c5a23b32021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10395https://doaj.org/toc/2076-3417Dental pulp inflammation, caused by the evolution of caries, involves numerous interrelated activities at a cellular and molecular level. Cytokines, proteases, growth factors, and other biomarkers of the host response may take part in dental pulp’s immune defense. The aim of this pilot study was to determine the levels of inflammation, oxidative stress, and extracellular matrix degradation biomarkers in healthy and symptomatic irreversibly inflamed dental pulp samples from children and adolescents. Twenty-three dental pulp samples were collected from permanent teeth with irreversible inflammation, while nineteen healthy dental pulp samples were obtained from teeth extracted for orthodontic reasons. Pulp lysates were obtained and the levels of IL-2, IL-17, TNF-α, SOD3, TGF-<i>β</i>1, catalase, osteocalcin, MMP-7, and MMP-9 were determined using the enzyme-linked immunosorbent assay (ELISA) technique. We detected significantly higher levels (<i>p</i> < 0.001) of IL-2, IL-17, TNF-α, SOD3, osteocalcin, and TGF-<i>β</i>1 in pulp samples with irreversible inflammation than in controls. Catalase and MMP-7 showed higher levels in the experimental group, while MMP-9 showed slightly increased levels in the control group, but none of these differences were statistically significant (<i>p</i> = 0.064/<i>p</i> = 0.061/<i>p</i> = 0.625). Inflamed dental pulp samples showed an up-regulation of IL-2, IL-17, TNF-α, SOD3, osteocalcin, and TGF-<i>β</i>1. These biomarkers appear to have a powerful role in the inflammation process of human dental pulp.Konstantina KritikouMarina ImreMihaela TanaseArina VinereanuAlexandra Ripszky TotanTudor-Claudiu SpinuRadu IlincaDaniela MiricescuIulia-Ioana Stanescu-SpinuMaria GreabuMDPI AGarticledental pulpinflammationbiomarkersenzyme-linked immunosorbent assayoxidative stressextracellular matrix degradationTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10395, p 10395 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dental pulp inflammation biomarkers enzyme-linked immunosorbent assay oxidative stress extracellular matrix degradation Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	dental pulp inflammation biomarkers enzyme-linked immunosorbent assay oxidative stress extracellular matrix degradation Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Konstantina Kritikou Marina Imre Mihaela Tanase Arina Vinereanu Alexandra Ripszky Totan Tudor-Claudiu Spinu Radu Ilinca Daniela Miricescu Iulia-Ioana Stanescu-Spinu Maria Greabu Biochemical Mapping of the Inflamed Human Dental Pulp
description	Dental pulp inflammation, caused by the evolution of caries, involves numerous interrelated activities at a cellular and molecular level. Cytokines, proteases, growth factors, and other biomarkers of the host response may take part in dental pulp’s immune defense. The aim of this pilot study was to determine the levels of inflammation, oxidative stress, and extracellular matrix degradation biomarkers in healthy and symptomatic irreversibly inflamed dental pulp samples from children and adolescents. Twenty-three dental pulp samples were collected from permanent teeth with irreversible inflammation, while nineteen healthy dental pulp samples were obtained from teeth extracted for orthodontic reasons. Pulp lysates were obtained and the levels of IL-2, IL-17, TNF-α, SOD3, TGF-<i>β</i>1, catalase, osteocalcin, MMP-7, and MMP-9 were determined using the enzyme-linked immunosorbent assay (ELISA) technique. We detected significantly higher levels (<i>p</i> < 0.001) of IL-2, IL-17, TNF-α, SOD3, osteocalcin, and TGF-<i>β</i>1 in pulp samples with irreversible inflammation than in controls. Catalase and MMP-7 showed higher levels in the experimental group, while MMP-9 showed slightly increased levels in the control group, but none of these differences were statistically significant (<i>p</i> = 0.064/<i>p</i> = 0.061/<i>p</i> = 0.625). Inflamed dental pulp samples showed an up-regulation of IL-2, IL-17, TNF-α, SOD3, osteocalcin, and TGF-<i>β</i>1. These biomarkers appear to have a powerful role in the inflammation process of human dental pulp.
format	article
author	Konstantina Kritikou Marina Imre Mihaela Tanase Arina Vinereanu Alexandra Ripszky Totan Tudor-Claudiu Spinu Radu Ilinca Daniela Miricescu Iulia-Ioana Stanescu-Spinu Maria Greabu
author_facet	Konstantina Kritikou Marina Imre Mihaela Tanase Arina Vinereanu Alexandra Ripszky Totan Tudor-Claudiu Spinu Radu Ilinca Daniela Miricescu Iulia-Ioana Stanescu-Spinu Maria Greabu
author_sort	Konstantina Kritikou
title	Biochemical Mapping of the Inflamed Human Dental Pulp
title_short	Biochemical Mapping of the Inflamed Human Dental Pulp
title_full	Biochemical Mapping of the Inflamed Human Dental Pulp
title_fullStr	Biochemical Mapping of the Inflamed Human Dental Pulp
title_full_unstemmed	Biochemical Mapping of the Inflamed Human Dental Pulp
title_sort	biochemical mapping of the inflamed human dental pulp
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24d93aa20fff436290be673a3c5a23b3
work_keys_str_mv	AT konstantinakritikou biochemicalmappingoftheinflamedhumandentalpulp AT marinaimre biochemicalmappingoftheinflamedhumandentalpulp AT mihaelatanase biochemicalmappingoftheinflamedhumandentalpulp AT arinavinereanu biochemicalmappingoftheinflamedhumandentalpulp AT alexandraripszkytotan biochemicalmappingoftheinflamedhumandentalpulp AT tudorclaudiuspinu biochemicalmappingoftheinflamedhumandentalpulp AT raduilinca biochemicalmappingoftheinflamedhumandentalpulp AT danielamiricescu biochemicalmappingoftheinflamedhumandentalpulp AT iuliaioanastanescuspinu biochemicalmappingoftheinflamedhumandentalpulp AT mariagreabu biochemicalmappingoftheinflamedhumandentalpulp
_version_	1718435355486584832

Biochemical Mapping of the Inflamed Human Dental Pulp

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