Gene expression-based biological test for major depressive disorder: an advanced study

Shin-ya Watanabe,1 Shusuke Numata,1 Jun-ichi Iga,2 Makoto Kinoshita,1 Hidehiro Umehara,1 Kazuo Ishii,3 Tetsuro Ohmori1 1Department of Psychiatry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 2Department of Neuropsychiatry, Molecules and Function, Ehime Universi...

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Autores principales:	Watanabe S, Numata S, Iga J, Kinoshita M, Umehara H, Ishii K, Ohmori T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	depressive disorder biomarker gene expression schizophrenia bipolar disorder Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/24dca6390f384e50bedaf99f7b4963d8
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spelling	oai:doaj.org-article:24dca6390f384e50bedaf99f7b4963d82021-12-02T05:54:03ZGene expression-based biological test for major depressive disorder: an advanced study1178-2021https://doaj.org/article/24dca6390f384e50bedaf99f7b4963d82017-02-01T00:00:00Zhttps://www.dovepress.com/gene-expression-based-biological-test-for-major-depressive-disorder-an-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Shin-ya Watanabe,1 Shusuke Numata,1 Jun-ichi Iga,2 Makoto Kinoshita,1 Hidehiro Umehara,1 Kazuo Ishii,3 Tetsuro Ohmori1 1Department of Psychiatry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 2Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Ehime, 3Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan Purpose: Recently, we could distinguished patients with major depressive disorder (MDD) from nonpsychiatric controls with high accuracy using a panel of five gene expression markers (ARHGAP24, HDAC5, PDGFC, PRNP, and SLC6A4) in leukocyte. In the present study, we examined whether this biological test is able to discriminate patients with MDD from those without MDD, including those with schizophrenia and bipolar disorder.Patients and methods: We measured messenger ribonucleic acid expression levels of the aforementioned five genes in peripheral leukocytes in 17 patients with schizophrenia and 36 patients with bipolar disorder using quantitative real-time polymerase chain reaction (PCR), and we combined these expression data with our previous expression data of 25 patients with MDD and 25 controls. Subsequently, a linear discriminant function was developed for use in discriminating between patients with MDD and without MDD.Results: This expression panel was able to segregate patients with MDD from those without MDD with a sensitivity and specificity of 64% and 67.9%, respectively.Conclusion: Further research to identify MDD-specific markers is needed to improve the performance of this biological test. Keywords: depressive disorder, biomarker, gene expression, schizophrenia, bipolar disorderWatanabe SNumata SIga JKinoshita MUmehara HIshii KOhmori TDove Medical Pressarticledepressive disorderbiomarkergene expressionschizophreniabipolar disorderNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 13, Pp 535-541 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	depressive disorder biomarker gene expression schizophrenia bipolar disorder Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	depressive disorder biomarker gene expression schizophrenia bipolar disorder Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Watanabe S Numata S Iga J Kinoshita M Umehara H Ishii K Ohmori T Gene expression-based biological test for major depressive disorder: an advanced study
description	Shin-ya Watanabe,1 Shusuke Numata,1 Jun-ichi Iga,2 Makoto Kinoshita,1 Hidehiro Umehara,1 Kazuo Ishii,3 Tetsuro Ohmori1 1Department of Psychiatry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 2Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Ehime, 3Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan Purpose: Recently, we could distinguished patients with major depressive disorder (MDD) from nonpsychiatric controls with high accuracy using a panel of five gene expression markers (ARHGAP24, HDAC5, PDGFC, PRNP, and SLC6A4) in leukocyte. In the present study, we examined whether this biological test is able to discriminate patients with MDD from those without MDD, including those with schizophrenia and bipolar disorder.Patients and methods: We measured messenger ribonucleic acid expression levels of the aforementioned five genes in peripheral leukocytes in 17 patients with schizophrenia and 36 patients with bipolar disorder using quantitative real-time polymerase chain reaction (PCR), and we combined these expression data with our previous expression data of 25 patients with MDD and 25 controls. Subsequently, a linear discriminant function was developed for use in discriminating between patients with MDD and without MDD.Results: This expression panel was able to segregate patients with MDD from those without MDD with a sensitivity and specificity of 64% and 67.9%, respectively.Conclusion: Further research to identify MDD-specific markers is needed to improve the performance of this biological test. Keywords: depressive disorder, biomarker, gene expression, schizophrenia, bipolar disorder
format	article
author	Watanabe S Numata S Iga J Kinoshita M Umehara H Ishii K Ohmori T
author_facet	Watanabe S Numata S Iga J Kinoshita M Umehara H Ishii K Ohmori T
author_sort	Watanabe S
title	Gene expression-based biological test for major depressive disorder: an advanced study
title_short	Gene expression-based biological test for major depressive disorder: an advanced study
title_full	Gene expression-based biological test for major depressive disorder: an advanced study
title_fullStr	Gene expression-based biological test for major depressive disorder: an advanced study
title_full_unstemmed	Gene expression-based biological test for major depressive disorder: an advanced study
title_sort	gene expression-based biological test for major depressive disorder: an advanced study
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/24dca6390f384e50bedaf99f7b4963d8
work_keys_str_mv	AT watanabes geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy AT numatas geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy AT igaj geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy AT kinoshitam geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy AT umeharah geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy AT ishiik geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy AT ohmorit geneexpressionbasedbiologicaltestformajordepressivedisorderanadvancedstudy
_version_	1718400160227131392

Gene expression-based biological test for major depressive disorder: an advanced study

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