The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish
Abstract Amyloid precursor protein (APP) is expressed in many tissues in human, mice and in zebrafish. In zebrafish, there are two orthologues, Appa and Appb. Interestingly, some cellular processes associated with APP overlap with cilia-mediated functions. Whereas the localization of APP to primary...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/24e291f60ef94c26ae05525f793703cf |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:24e291f60ef94c26ae05525f793703cf |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:24e291f60ef94c26ae05525f793703cf2021-12-02T17:17:40ZThe localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish10.1038/s41598-021-98487-72045-2322https://doaj.org/article/24e291f60ef94c26ae05525f793703cf2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98487-7https://doaj.org/toc/2045-2322Abstract Amyloid precursor protein (APP) is expressed in many tissues in human, mice and in zebrafish. In zebrafish, there are two orthologues, Appa and Appb. Interestingly, some cellular processes associated with APP overlap with cilia-mediated functions. Whereas the localization of APP to primary cilia of in vitro-cultured cells has been reported, we addressed the presence of APP in motile and in non-motile sensory cilia and its potential implication for ciliogenesis using zebrafish, mouse, and human samples. We report that Appa and Appb are expressed by ciliated cells and become localized at the membrane of cilia in the olfactory epithelium, otic vesicle and in the brain ventricles of zebrafish embryos. App in ependymal cilia persisted in adult zebrafish and was also detected in mouse and human brain. Finally, we found morphologically abnormal ependymal cilia and smaller brain ventricles in appa −/− appb −/− mutant zebrafish. Our findings demonstrate an evolutionary conserved localisation of APP to cilia and suggest a role of App in ciliogenesis and cilia-related functions.Jasmine ChebliMaryam RahmatiTammaryn LashleyBrigitta EdemanAnders OldforsHenrik ZetterbergAlexandra AbramssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Jasmine Chebli Maryam Rahmati Tammaryn Lashley Brigitta Edeman Anders Oldfors Henrik Zetterberg Alexandra Abramsson The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| description |
Abstract Amyloid precursor protein (APP) is expressed in many tissues in human, mice and in zebrafish. In zebrafish, there are two orthologues, Appa and Appb. Interestingly, some cellular processes associated with APP overlap with cilia-mediated functions. Whereas the localization of APP to primary cilia of in vitro-cultured cells has been reported, we addressed the presence of APP in motile and in non-motile sensory cilia and its potential implication for ciliogenesis using zebrafish, mouse, and human samples. We report that Appa and Appb are expressed by ciliated cells and become localized at the membrane of cilia in the olfactory epithelium, otic vesicle and in the brain ventricles of zebrafish embryos. App in ependymal cilia persisted in adult zebrafish and was also detected in mouse and human brain. Finally, we found morphologically abnormal ependymal cilia and smaller brain ventricles in appa −/− appb −/− mutant zebrafish. Our findings demonstrate an evolutionary conserved localisation of APP to cilia and suggest a role of App in ciliogenesis and cilia-related functions. |
| format |
article |
| author |
Jasmine Chebli Maryam Rahmati Tammaryn Lashley Brigitta Edeman Anders Oldfors Henrik Zetterberg Alexandra Abramsson |
| author_facet |
Jasmine Chebli Maryam Rahmati Tammaryn Lashley Brigitta Edeman Anders Oldfors Henrik Zetterberg Alexandra Abramsson |
| author_sort |
Jasmine Chebli |
| title |
The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| title_short |
The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| title_full |
The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| title_fullStr |
The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| title_full_unstemmed |
The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| title_sort |
localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/24e291f60ef94c26ae05525f793703cf |
| work_keys_str_mv |
AT jasminechebli thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT maryamrahmati thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT tammarynlashley thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT brigittaedeman thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT andersoldfors thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT henrikzetterberg thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT alexandraabramsson thelocalizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT jasminechebli localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT maryamrahmati localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT tammarynlashley localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT brigittaedeman localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT andersoldfors localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT henrikzetterberg localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish AT alexandraabramsson localizationofamyloidprecursorproteintoependymalciliainvertebratesanditsroleinciliogenesisandbraindevelopmentinzebrafish |
| _version_ |
1718381132504891392 |