Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia

Abstract. Firawati I, Murwantoko, Setyobudi E. 2017. Morphological and molecular characterization of hairtail (Trichiurus spp.) from Indian Ocean, southern coast of East Java, Indonesia. Biodiversitas 18: 190-196. The objective of this study was to identify species of hairtails (Trichiurus spp.) whi...

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Autores principales:	IKA FIRAWATI, MURWANTOKO MURWANTOKO, EKO SETYOBUDI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	16s rrna gene, identification, molecular, morphometric, trichiurus spp. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/24e2f802c2884929a635bee479ee27e0
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spelling	oai:doaj.org-article:24e2f802c2884929a635bee479ee27e02021-11-16T13:50:45ZMorphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia1412-033X2085-472210.13057/biodiv/d180125https://doaj.org/article/24e2f802c2884929a635bee479ee27e02017-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/768https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Firawati I, Murwantoko, Setyobudi E. 2017. Morphological and molecular characterization of hairtail (Trichiurus spp.) from Indian Ocean, southern coast of East Java, Indonesia. Biodiversitas 18: 190-196. The objective of this study was to identify species of hairtails (Trichiurus spp.) which caught from the southern coast of East Java, based on morphology and molecular characters. Three hundred and forty-nine fish samples were collected from two fishing port i.e. Muncar and Prigi. Twenty-two morphometric and four meristic characters were measured from each sample. The result showed that the hairtail samples consisted of two species, T. lepturus and T. brevis based on morphology characters. Based on Principal Component Analysis (PCA) and Discriminant Function Analysis (DFA), these species can be distinguished by the head and caudal character, which the length of an upper jaw and caudal peduncle characters were the main distinguish. Analysis of fragment (~600 bp) of the mitochondrial DNA encoding 16S rRNA gene showed that T. lepturus samples were 100% genetically identical with T. lepturus while T. brevis samples were 100% identical with T. brevis. Phylogenetic analysis showed that T. lepturus and T. brevis were distinct species while T. lepturus both which caught from Prigi and Muncar were closely related species. The molecular identification can be used to verify the result of morphological identification. Key words: 16S rRNA gene, identification, molecular, morphometric, Trichiurus spp.IKA FIRAWATIMURWANTOKO MURWANTOKOEKO SETYOBUDIMBI & UNS Soloarticle16s rrna gene, identification, molecular, morphometric, trichiurus spp.Biology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene, identification, molecular, morphometric, trichiurus spp. Biology (General) QH301-705.5
spellingShingle	16s rrna gene, identification, molecular, morphometric, trichiurus spp. Biology (General) QH301-705.5 IKA FIRAWATI MURWANTOKO MURWANTOKO EKO SETYOBUDI Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia
description	Abstract. Firawati I, Murwantoko, Setyobudi E. 2017. Morphological and molecular characterization of hairtail (Trichiurus spp.) from Indian Ocean, southern coast of East Java, Indonesia. Biodiversitas 18: 190-196. The objective of this study was to identify species of hairtails (Trichiurus spp.) which caught from the southern coast of East Java, based on morphology and molecular characters. Three hundred and forty-nine fish samples were collected from two fishing port i.e. Muncar and Prigi. Twenty-two morphometric and four meristic characters were measured from each sample. The result showed that the hairtail samples consisted of two species, T. lepturus and T. brevis based on morphology characters. Based on Principal Component Analysis (PCA) and Discriminant Function Analysis (DFA), these species can be distinguished by the head and caudal character, which the length of an upper jaw and caudal peduncle characters were the main distinguish. Analysis of fragment (~600 bp) of the mitochondrial DNA encoding 16S rRNA gene showed that T. lepturus samples were 100% genetically identical with T. lepturus while T. brevis samples were 100% identical with T. brevis. Phylogenetic analysis showed that T. lepturus and T. brevis were distinct species while T. lepturus both which caught from Prigi and Muncar were closely related species. The molecular identification can be used to verify the result of morphological identification. Key words: 16S rRNA gene, identification, molecular, morphometric, Trichiurus spp.
format	article
author	IKA FIRAWATI MURWANTOKO MURWANTOKO EKO SETYOBUDI
author_facet	IKA FIRAWATI MURWANTOKO MURWANTOKO EKO SETYOBUDI
author_sort	IKA FIRAWATI
title	Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia
title_short	Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia
title_full	Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia
title_fullStr	Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia
title_full_unstemmed	Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia
title_sort	morphological and molecular characterization of hairtail (trichiurus spp.) from the indian ocean, southern coast of east java, indonesia
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/24e2f802c2884929a635bee479ee27e0
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Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia

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