Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains

Wei Hong, Lipeng Liu, Zehui Zhang, Yining Zhao, Dexian Zhang, Mingchun Liu Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, People’s Republic of China Background: Mult...

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Autores principales:	Hong W, Liu L, Zhang Z, Zhao Y, Zhang D, Liu M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	PEGylated Nano-BA12K multidrug-resistant Streptococcus pneumonia Penicillin-sensitive and -resistant. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/24e493249d6b4917967eaf0cd7e69aa8
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spelling	oai:doaj.org-article:24e493249d6b4917967eaf0cd7e69aa82021-12-02T02:22:34ZInsights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains1178-2013https://doaj.org/article/24e493249d6b4917967eaf0cd7e69aa82018-10-01T00:00:00Zhttps://www.dovepress.com/insights-into-the-antibacterial-mechanism-of-peg-ylated-nano-bacitraci-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Hong, Lipeng Liu, Zehui Zhang, Yining Zhao, Dexian Zhang, Mingchun Liu Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, People’s Republic of China Background: Multidrug-resistant (MDR) Streptococcus pneumonia constitute a major worldwide public health concern. Materials and methods: In our preliminary study, PEGylated nano-self-assemblies of bacitracin A (PEGylated Nano-BA12K) showed strong antibacterial potency against reference S. pneumonia strain (ATCC 49619). In this study, the possibility of applying PEGylated Nano-BA12K against penicillin-resistant S. pneumonia was further investigated. In addition, the underlying antibacterial mechanism of PEGylated Nano-BA12K against both sensitive and resistant S. pneumonia was also clarified systematically, since S. pneumonia was naturally resistant to its unassembled counterpart bacitracin A (BA). Results: PEGylated Nano-BA12K showed strong antibacterial potency against 13 clinical isolates of S. pneumonia, including five penicillin-resistant strains. Structural changes, partial collapse, and even lysis of both penicillin-sensitive and penicillin-resistant bacteria were observed after incubation with PEGylated Nano-BA12K via transmission electron microscopy and atomic force microscopy. Thus, the cell wall or/and cell membrane might be the main target of PEGylated Nano-BA12K against S. pneumonia. PEGylated Nano-BA12K exhibited limited effect on the permeabilization and peptidoglycan content of cell wall. Surface pressure measurement suggested that PEGylated Nano-BA12K was much more tensioactive than BA, which was usually translated into a good membranolytic effect, and is helpful to permeabilize the cell membrane and damage membrane integrity, as evidenced by depolarization of the membrane potential, permeabilization of membrane and leakage of calcein from liposomes. Conclusion: Collectively, great cell membrane permeability and formidable membrane disruption may work together for the strong antibacterial activity of PEGylated Nano-BA12K against S. pneumonia. Taken together, PEGylated Nano-BA12K has excellent potential against both penicillin-sensitive and penicillin-resistant S. pneumonia and might be suitable for the treatment of S. pneumonia infectious diseases. Keywords: PEGylated Nano-BA12K, multidrug-resistant, Streptococcus pneumonia, penicillin-sensitive and penicillin-resistantHong WLiu LZhang ZZhao YZhang DLiu MDove Medical PressarticlePEGylated Nano-BA12Kmultidrug-resistantStreptococcus pneumoniaPenicillin-sensitive and -resistant.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6297-6309 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	PEGylated Nano-BA12K multidrug-resistant Streptococcus pneumonia Penicillin-sensitive and -resistant. Medicine (General) R5-920
spellingShingle	PEGylated Nano-BA12K multidrug-resistant Streptococcus pneumonia Penicillin-sensitive and -resistant. Medicine (General) R5-920 Hong W Liu L Zhang Z Zhao Y Zhang D Liu M Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
description	Wei Hong, Lipeng Liu, Zehui Zhang, Yining Zhao, Dexian Zhang, Mingchun Liu Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, People’s Republic of China Background: Multidrug-resistant (MDR) Streptococcus pneumonia constitute a major worldwide public health concern. Materials and methods: In our preliminary study, PEGylated nano-self-assemblies of bacitracin A (PEGylated Nano-BA12K) showed strong antibacterial potency against reference S. pneumonia strain (ATCC 49619). In this study, the possibility of applying PEGylated Nano-BA12K against penicillin-resistant S. pneumonia was further investigated. In addition, the underlying antibacterial mechanism of PEGylated Nano-BA12K against both sensitive and resistant S. pneumonia was also clarified systematically, since S. pneumonia was naturally resistant to its unassembled counterpart bacitracin A (BA). Results: PEGylated Nano-BA12K showed strong antibacterial potency against 13 clinical isolates of S. pneumonia, including five penicillin-resistant strains. Structural changes, partial collapse, and even lysis of both penicillin-sensitive and penicillin-resistant bacteria were observed after incubation with PEGylated Nano-BA12K via transmission electron microscopy and atomic force microscopy. Thus, the cell wall or/and cell membrane might be the main target of PEGylated Nano-BA12K against S. pneumonia. PEGylated Nano-BA12K exhibited limited effect on the permeabilization and peptidoglycan content of cell wall. Surface pressure measurement suggested that PEGylated Nano-BA12K was much more tensioactive than BA, which was usually translated into a good membranolytic effect, and is helpful to permeabilize the cell membrane and damage membrane integrity, as evidenced by depolarization of the membrane potential, permeabilization of membrane and leakage of calcein from liposomes. Conclusion: Collectively, great cell membrane permeability and formidable membrane disruption may work together for the strong antibacterial activity of PEGylated Nano-BA12K against S. pneumonia. Taken together, PEGylated Nano-BA12K has excellent potential against both penicillin-sensitive and penicillin-resistant S. pneumonia and might be suitable for the treatment of S. pneumonia infectious diseases. Keywords: PEGylated Nano-BA12K, multidrug-resistant, Streptococcus pneumonia, penicillin-sensitive and penicillin-resistant
format	article
author	Hong W Liu L Zhang Z Zhao Y Zhang D Liu M
author_facet	Hong W Liu L Zhang Z Zhao Y Zhang D Liu M
author_sort	Hong W
title	Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
title_short	Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
title_full	Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
title_fullStr	Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
title_full_unstemmed	Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
title_sort	insights into the antibacterial mechanism of pegylated nano-bacitracin a against streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/24e493249d6b4917967eaf0cd7e69aa8
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Insights into the antibacterial mechanism of PEGylated nano-bacitracin A against Streptococcus pneumonia: both penicillin-sensitive and penicillin-resistant strains

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