Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection

Abstract Developing point-of-care (PoC) diagnostic platforms for carcinoembryonic antigen detection is essential. However, thefew implementations of transferring the signal amplification strategies in electrochemical sensing on paper-based platforms are not satisfactory in terms of detection limit (...

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Autores principales:	B. Chakraborty, A. Das, N. Mandal, N. Samanta, N. Das, C. Roy Chaudhuri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24e867c7a3a94375bdd1fd48058d9d84
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spelling	oai:doaj.org-article:24e867c7a3a94375bdd1fd48058d9d842021-12-02T14:06:56ZLabel free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection10.1038/s41598-021-82580-y2045-2322https://doaj.org/article/24e867c7a3a94375bdd1fd48058d9d842021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82580-yhttps://doaj.org/toc/2045-2322Abstract Developing point-of-care (PoC) diagnostic platforms for carcinoembryonic antigen detection is essential. However, thefew implementations of transferring the signal amplification strategies in electrochemical sensing on paper-based platforms are not satisfactory in terms of detection limit (LOD). In the quest for pushing down LOD, majority of the research has been targeted towards development of improved nanostructured substrates for entrapping more analyte molecules and augmenting the electron transfer rate to the working electrode. But, such approaches have reached saturation. This paper focuses on enhancing the mass transport of the analyte towards the sensor surface through the application of an electric field, in graphene-ZnO nanorods heterostructure. These hybrid nanostructures have been deposited on flexible polyethylene terephthalate substrates with screen printed electrodes for PoC application. The ZnO nanorods have been functionalized with aptamers and the working sensor has been integrated with smartphone interfaced indigenously developed low cost potentiostat. The performance of the system, requiring only 50 µl analyte has been evaluated using electrochemical impedance spectroscopy and validated against commercially available ELISA kit. Limit of detection of 1 fg/ml in human serum with 6.5% coefficient of variation has been demonstrated, which is more than three orders of magnitude lower than the existing attempts on PoC device.B. ChakrabortyA. DasN. MandalN. SamantaN. DasC. Roy ChaudhuriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q B. Chakraborty A. Das N. Mandal N. Samanta N. Das C. Roy Chaudhuri Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
description	Abstract Developing point-of-care (PoC) diagnostic platforms for carcinoembryonic antigen detection is essential. However, thefew implementations of transferring the signal amplification strategies in electrochemical sensing on paper-based platforms are not satisfactory in terms of detection limit (LOD). In the quest for pushing down LOD, majority of the research has been targeted towards development of improved nanostructured substrates for entrapping more analyte molecules and augmenting the electron transfer rate to the working electrode. But, such approaches have reached saturation. This paper focuses on enhancing the mass transport of the analyte towards the sensor surface through the application of an electric field, in graphene-ZnO nanorods heterostructure. These hybrid nanostructures have been deposited on flexible polyethylene terephthalate substrates with screen printed electrodes for PoC application. The ZnO nanorods have been functionalized with aptamers and the working sensor has been integrated with smartphone interfaced indigenously developed low cost potentiostat. The performance of the system, requiring only 50 µl analyte has been evaluated using electrochemical impedance spectroscopy and validated against commercially available ELISA kit. Limit of detection of 1 fg/ml in human serum with 6.5% coefficient of variation has been demonstrated, which is more than three orders of magnitude lower than the existing attempts on PoC device.
format	article
author	B. Chakraborty A. Das N. Mandal N. Samanta N. Das C. Roy Chaudhuri
author_facet	B. Chakraborty A. Das N. Mandal N. Samanta N. Das C. Roy Chaudhuri
author_sort	B. Chakraborty
title	Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
title_short	Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
title_full	Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
title_fullStr	Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
title_full_unstemmed	Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
title_sort	label free, electric field mediated ultrasensitive electrochemical point-of-care device for cea detection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/24e867c7a3a94375bdd1fd48058d9d84
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Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection

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