Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil

Context: Cola gigantea A. Chev. (Sterculiaceae) is an important medicinal tropical flora. Objective: The seed oil of C. gigantea, an underutilized tropical plant was investigated for its antioxidant, anti-inflammatory, anti-Toxoplasma, and cytotoxicity activities as well as the chemical composition....

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Autores principales:	O. Atolani, H. Oguntoye, E. T. Areh, O. S. Adeyemi, L. Kambizi
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	fatty acid toxoplasma gondii fibroblast sterolic acid stigmasterol β-sitosterol dpph abts cell membrane stabilization Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/24eb4f7106f84711b921c43fc29d0509
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spelling	oai:doaj.org-article:24eb4f7106f84711b921c43fc29d05092021-11-17T14:21:55ZChemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil1388-02091744-511610.1080/13880209.2019.1577468https://doaj.org/article/24eb4f7106f84711b921c43fc29d05092019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1577468https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Cola gigantea A. Chev. (Sterculiaceae) is an important medicinal tropical flora. Objective: The seed oil of C. gigantea, an underutilized tropical plant was investigated for its antioxidant, anti-inflammatory, anti-Toxoplasma, and cytotoxicity activities as well as the chemical composition. Materials and methods: The physicochemical parameters of the seed oil obtained via Soxhlet extraction was determined while the fatty acid and non-fatty acid component were analyzed by gas chromatography-mass spectrometry. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays (10–50 µg/mL) while the anti-inflammatory property was determined through Cell Membrane Stabilization assay. The anti-parasite and cytotoxicity activity were evaluated (0–1000 µg/mL) using Toxoplasma gondii and mammalian cell line assays, respectively. Results: The oil had fatty acids which ranged from C-12 to C-23 with linoleic (18:2) and palmitic acids (16:0) being dominant. The oil had 89.41% unsaturated fatty acids with sterolic acid, an uncommon acetylenic fatty acid reported for the first time. Non-fatty acids obtained include cholesterol (2.12%), campesterol (14.12%), stigmasterol (34.07%) and β-sitosterol (49.68%). The oil had a significantly (p < 0.05) low scavenging activity against DPPH radicals (IC50 > 50 µg/mL) compared with ascorbic acid. In contrast, the oil showed better activity against ABTS radicals (IC50 44.19 ± 6.27 µg/mL) compared with ascorbic acid or quercetin. Furthermore, the oil showed anti-T. gondii and dose-dependent cytotoxicity in HFF cells with selectivity index (IC50/EC50 < 1). Discussion and conclusions: The antioxidant potential of the oil suggests that it may serve as a potential source for various preparations for pharmaceuticals and cosmeceuticals.O. AtolaniH. OguntoyeE. T. ArehO. S. AdeyemiL. KambiziTaylor & Francis Grouparticlefatty acidtoxoplasma gondiifibroblaststerolic acidstigmasterolβ-sitosteroldpphabtscell membrane stabilizationTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 154-160 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	fatty acid toxoplasma gondii fibroblast sterolic acid stigmasterol β-sitosterol dpph abts cell membrane stabilization Therapeutics. Pharmacology RM1-950
spellingShingle	fatty acid toxoplasma gondii fibroblast sterolic acid stigmasterol β-sitosterol dpph abts cell membrane stabilization Therapeutics. Pharmacology RM1-950 O. Atolani H. Oguntoye E. T. Areh O. S. Adeyemi L. Kambizi Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil
description	Context: Cola gigantea A. Chev. (Sterculiaceae) is an important medicinal tropical flora. Objective: The seed oil of C. gigantea, an underutilized tropical plant was investigated for its antioxidant, anti-inflammatory, anti-Toxoplasma, and cytotoxicity activities as well as the chemical composition. Materials and methods: The physicochemical parameters of the seed oil obtained via Soxhlet extraction was determined while the fatty acid and non-fatty acid component were analyzed by gas chromatography-mass spectrometry. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays (10–50 µg/mL) while the anti-inflammatory property was determined through Cell Membrane Stabilization assay. The anti-parasite and cytotoxicity activity were evaluated (0–1000 µg/mL) using Toxoplasma gondii and mammalian cell line assays, respectively. Results: The oil had fatty acids which ranged from C-12 to C-23 with linoleic (18:2) and palmitic acids (16:0) being dominant. The oil had 89.41% unsaturated fatty acids with sterolic acid, an uncommon acetylenic fatty acid reported for the first time. Non-fatty acids obtained include cholesterol (2.12%), campesterol (14.12%), stigmasterol (34.07%) and β-sitosterol (49.68%). The oil had a significantly (p < 0.05) low scavenging activity against DPPH radicals (IC50 > 50 µg/mL) compared with ascorbic acid. In contrast, the oil showed better activity against ABTS radicals (IC50 44.19 ± 6.27 µg/mL) compared with ascorbic acid or quercetin. Furthermore, the oil showed anti-T. gondii and dose-dependent cytotoxicity in HFF cells with selectivity index (IC50/EC50 < 1). Discussion and conclusions: The antioxidant potential of the oil suggests that it may serve as a potential source for various preparations for pharmaceuticals and cosmeceuticals.
format	article
author	O. Atolani H. Oguntoye E. T. Areh O. S. Adeyemi L. Kambizi
author_facet	O. Atolani H. Oguntoye E. T. Areh O. S. Adeyemi L. Kambizi
author_sort	O. Atolani
title	Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil
title_short	Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil
title_full	Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil
title_fullStr	Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil
title_full_unstemmed	Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil
title_sort	chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of cola gigantea seed oil
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/24eb4f7106f84711b921c43fc29d0509
work_keys_str_mv	AT oatolani chemicalcompositionantitoxoplasmacytotoxicityantioxidantandantiinflammatorypotentialsofcolagiganteaseedoil AT hoguntoye chemicalcompositionantitoxoplasmacytotoxicityantioxidantandantiinflammatorypotentialsofcolagiganteaseedoil AT etareh chemicalcompositionantitoxoplasmacytotoxicityantioxidantandantiinflammatorypotentialsofcolagiganteaseedoil AT osadeyemi chemicalcompositionantitoxoplasmacytotoxicityantioxidantandantiinflammatorypotentialsofcolagiganteaseedoil AT lkambizi chemicalcompositionantitoxoplasmacytotoxicityantioxidantandantiinflammatorypotentialsofcolagiganteaseedoil
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Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil

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