Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.

N-methyl-D-aspartate (NMDA) receptors are associated with many forms of synaptic plasticity. Their expression level and subunit composition undergo developmental changes in several brain regions. In the mouse cerebellum, beside a developmental switch between NR2B and NR2A/C subunits in granule cells...

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Autores principales:	Etienne Lonchamp, Frédéric Gambino, Jean Luc Dupont, Frédéric Doussau, Antoine Valera, Bernard Poulain, Jean-Louis Bossu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24eba025e7c44e47ae75d6d65b50445e
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spelling	oai:doaj.org-article:24eba025e7c44e47ae75d6d65b50445e2021-11-18T07:29:49ZPre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.1932-620310.1371/journal.pone.0030180https://doaj.org/article/24eba025e7c44e47ae75d6d65b50445e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22276158/?tool=EBIhttps://doaj.org/toc/1932-6203N-methyl-D-aspartate (NMDA) receptors are associated with many forms of synaptic plasticity. Their expression level and subunit composition undergo developmental changes in several brain regions. In the mouse cerebellum, beside a developmental switch between NR2B and NR2A/C subunits in granule cells, functional postsynaptic NMDA receptors are seen in Purkinje cells of neonate and adult but not juvenile rat and mice. A presynaptic effect of NMDA on GABA release by cerebellar interneurons was identified recently. Nevertheless whereas NMDA receptor subunits are detected on parallel fiber terminals, a presynaptic effect of NMDA on spontaneous release of glutamate has not been demonstrated. Using mouse cerebellar cultures and patch-clamp recordings we show that NMDA facilitates glutamate release onto Purkinje cells in young cultures via a presynaptic mechanism, whereas NMDA activates extrasynaptic receptors in Purkinje cells recorded in old cultures. The presynaptic effect of NMDA on glutamate release is also observed in Purkinje cells recorded in acute slices prepared from juvenile but not from adult mice and requires a specific protocol of NMDA application.Etienne LonchampFrédéric GambinoJean Luc DupontFrédéric DoussauAntoine ValeraBernard PoulainJean-Louis BossuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30180 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Etienne Lonchamp Frédéric Gambino Jean Luc Dupont Frédéric Doussau Antoine Valera Bernard Poulain Jean-Louis Bossu Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.
description	N-methyl-D-aspartate (NMDA) receptors are associated with many forms of synaptic plasticity. Their expression level and subunit composition undergo developmental changes in several brain regions. In the mouse cerebellum, beside a developmental switch between NR2B and NR2A/C subunits in granule cells, functional postsynaptic NMDA receptors are seen in Purkinje cells of neonate and adult but not juvenile rat and mice. A presynaptic effect of NMDA on GABA release by cerebellar interneurons was identified recently. Nevertheless whereas NMDA receptor subunits are detected on parallel fiber terminals, a presynaptic effect of NMDA on spontaneous release of glutamate has not been demonstrated. Using mouse cerebellar cultures and patch-clamp recordings we show that NMDA facilitates glutamate release onto Purkinje cells in young cultures via a presynaptic mechanism, whereas NMDA activates extrasynaptic receptors in Purkinje cells recorded in old cultures. The presynaptic effect of NMDA on glutamate release is also observed in Purkinje cells recorded in acute slices prepared from juvenile but not from adult mice and requires a specific protocol of NMDA application.
format	article
author	Etienne Lonchamp Frédéric Gambino Jean Luc Dupont Frédéric Doussau Antoine Valera Bernard Poulain Jean-Louis Bossu
author_facet	Etienne Lonchamp Frédéric Gambino Jean Luc Dupont Frédéric Doussau Antoine Valera Bernard Poulain Jean-Louis Bossu
author_sort	Etienne Lonchamp
title	Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.
title_short	Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.
title_full	Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.
title_fullStr	Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.
title_full_unstemmed	Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.
title_sort	pre and post synaptic nmda effects targeting purkinje cells in the mouse cerebellar cortex.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/24eba025e7c44e47ae75d6d65b50445e
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Pre and post synaptic NMDA effects targeting Purkinje cells in the mouse cerebellar cortex.

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