Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation

Nan Jiang, Shuaiwei Zhao, Shilei Wang, Zhong Lu Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of ChinaCorrespondence: Zhong LuWuhan Institute of Technolog...

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Autores principales:	Jiang N, Zhao S, Wang S, Lu Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	antibiofilm protein profiling nanoparticles s. mutans biofilm-associated diseases Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/24ec8c5ab8c94735a27ca6a622263790
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spelling	oai:doaj.org-article:24ec8c5ab8c94735a27ca6a6222637902021-12-02T18:47:23ZProteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation1178-2013https://doaj.org/article/24ec8c5ab8c94735a27ca6a6222637902021-11-01T00:00:00Zhttps://www.dovepress.com/proteomics-of-streptococcus-mutans-to-reveal-the-antibiofilm-formation-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Nan Jiang, Shuaiwei Zhao, Shilei Wang, Zhong Lu Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of ChinaCorrespondence: Zhong LuWuhan Institute of Technology, Wuhan, 430205, People’s Republic of ChinaTel +86-18062036269Fax +86-27-67905262Email zhonglu@wit.edu.cnIntroduction: As a biofilm-associated disease, dental caries benefits from nanoparticle (NP)-based therapies. Streptococcus mutans (S. mutans) is a primary aetiologic agent for dental caries development. We successfully applied a synergistic therapy of Ag/ZnO nanocomposites combined with light-emitting diode (LED) radiation to inhibit S. mutans biofilms. However, the antibiofilm mechanism has not been fully elucidated, and little is known about the biofilm formation ability of bacteria that survive NP-based therapies.Methods: This study explored the antibiofilm formation mechanism of this synergistic therapy by an integrated approach based upon proteomics.Results: Synergistic therapy killed 99.8% of bacteria, while the biofilm formation ability of 0.2% surviving bacteria was inhibited. The proteomic responses of S. mutans to synergistic therapy were comprehensively characterized to unveil the mechanism of bacterial death and biofilm formation inhibition of the surviving bacteria. In total, 55 differentially expressed proteins (12 upregulated and 43 downregulated) were recorded. The bioinformatic analysis demonstrated that cellular integrity damage and regulated expression of structure-associated proteins were the main reasons for bacterial death. In addition, the proteomic study indicated the potential inhibition of metabolism in surviving bacteria and provided a biofilm-related network consisting of 17 differentially expressed proteins, explaining the multiantibiofilm formation actions. Finally, we reported and verified the inhibitory effects of synergistic therapy on sucrose metabolism and D-alanine metabolism, which disturbed the biofilm formation of surviving bacteria.Conclusion: Our findings demonstrated that synergistic therapy killed most bacteria and inhibited the surviving bacteria from forming biofilms. Furthermore, the antibiofilm formation mechanism was revealed by proteomics analysis of S. mutans after synergistic therapy and subsequent metabolic studies. Our success may provide a showcase to explore the antibiofilm formation mechanism of NP-based therapies using proteomic studies.Keywords: antibiofilm, protein profiling, nanoparticles, S. mutans, biofilm-associated diseasesJiang NZhao SWang SLu ZDove Medical Pressarticleantibiofilmprotein profilingnanoparticless. mutansbiofilm-associated diseasesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 7741-7757 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiofilm protein profiling nanoparticles s. mutans biofilm-associated diseases Medicine (General) R5-920
spellingShingle	antibiofilm protein profiling nanoparticles s. mutans biofilm-associated diseases Medicine (General) R5-920 Jiang N Zhao S Wang S Lu Z Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation
description	Nan Jiang, Shuaiwei Zhao, Shilei Wang, Zhong Lu Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of ChinaCorrespondence: Zhong LuWuhan Institute of Technology, Wuhan, 430205, People’s Republic of ChinaTel +86-18062036269Fax +86-27-67905262Email zhonglu@wit.edu.cnIntroduction: As a biofilm-associated disease, dental caries benefits from nanoparticle (NP)-based therapies. Streptococcus mutans (S. mutans) is a primary aetiologic agent for dental caries development. We successfully applied a synergistic therapy of Ag/ZnO nanocomposites combined with light-emitting diode (LED) radiation to inhibit S. mutans biofilms. However, the antibiofilm mechanism has not been fully elucidated, and little is known about the biofilm formation ability of bacteria that survive NP-based therapies.Methods: This study explored the antibiofilm formation mechanism of this synergistic therapy by an integrated approach based upon proteomics.Results: Synergistic therapy killed 99.8% of bacteria, while the biofilm formation ability of 0.2% surviving bacteria was inhibited. The proteomic responses of S. mutans to synergistic therapy were comprehensively characterized to unveil the mechanism of bacterial death and biofilm formation inhibition of the surviving bacteria. In total, 55 differentially expressed proteins (12 upregulated and 43 downregulated) were recorded. The bioinformatic analysis demonstrated that cellular integrity damage and regulated expression of structure-associated proteins were the main reasons for bacterial death. In addition, the proteomic study indicated the potential inhibition of metabolism in surviving bacteria and provided a biofilm-related network consisting of 17 differentially expressed proteins, explaining the multiantibiofilm formation actions. Finally, we reported and verified the inhibitory effects of synergistic therapy on sucrose metabolism and D-alanine metabolism, which disturbed the biofilm formation of surviving bacteria.Conclusion: Our findings demonstrated that synergistic therapy killed most bacteria and inhibited the surviving bacteria from forming biofilms. Furthermore, the antibiofilm formation mechanism was revealed by proteomics analysis of S. mutans after synergistic therapy and subsequent metabolic studies. Our success may provide a showcase to explore the antibiofilm formation mechanism of NP-based therapies using proteomic studies.Keywords: antibiofilm, protein profiling, nanoparticles, S. mutans, biofilm-associated diseases
format	article
author	Jiang N Zhao S Wang S Lu Z
author_facet	Jiang N Zhao S Wang S Lu Z
author_sort	Jiang N
title	Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation
title_short	Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation
title_full	Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation
title_fullStr	Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation
title_full_unstemmed	Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation
title_sort	proteomics of streptococcus mutans to reveal the antibiofilm formation mechanism of ag/zno nanocomposites with light-emitting diode radiation
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/24ec8c5ab8c94735a27ca6a622263790
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Proteomics of Streptococcus mutans to Reveal the Antibiofilm Formation Mechanism of Ag/ZnO Nanocomposites with Light-Emitting Diode Radiation

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