Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.

Acinetobacter baumannii is a common pathogen whose recent resistance to drugs has emerged as a major health problem. Ethanol has been found to increase the virulence of A. baumannii in Dictyostelium discoideum and Caenorhabditis elegans models of infection. To better understand the causes of this ef...

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Autores principales:	Laura Camarena, Vincent Bruno, Ghia Euskirchen, Sebastian Poggio, Michael Snyder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/24f6110ba317460a91e99f402a51e5c9
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spelling	oai:doaj.org-article:24f6110ba317460a91e99f402a51e5c92021-11-25T05:48:11ZMolecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.1553-73661553-737410.1371/journal.ppat.1000834https://doaj.org/article/24f6110ba317460a91e99f402a51e5c92010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20368969/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Acinetobacter baumannii is a common pathogen whose recent resistance to drugs has emerged as a major health problem. Ethanol has been found to increase the virulence of A. baumannii in Dictyostelium discoideum and Caenorhabditis elegans models of infection. To better understand the causes of this effect, we examined the transcriptional profile of A. baumannii grown in the presence or absence of ethanol using RNA-Seq. Using the Illumina/Solexa platform, a total of 43,453,960 reads (35 nt) were obtained, of which 3,596,474 mapped uniquely to the genome. Our analysis revealed that ethanol induces the expression of 49 genes that belong to different functional categories. A strong induction was observed for genes encoding metabolic enzymes, indicating that ethanol is efficiently assimilated. In addition, we detected the induction of genes encoding stress proteins, including upsA, hsp90, groEL and lon as well as permeases, efflux pumps and a secreted phospholipase C. In stationary phase, ethanol strongly induced several genes involved with iron assimilation and a high-affinity phosphate transport system, indicating that A. baumannii makes a better use of the iron and phosphate resources in the medium when ethanol is used as a carbon source. To evaluate the role of phospholipase C (Plc1) in virulence, we generated and analyzed a deletion mutant for plc1. This strain exhibits a modest, but reproducible, reduction in the cytotoxic effect caused by A. baumannii on epithelial cells, suggesting that phospholipase C is important for virulence. Overall, our results indicate the power of applying RNA-Seq to identify key modulators of bacterial pathogenesis. We suggest that the effect of ethanol on the virulence of A. baumannii is multifactorial and includes a general stress response and other specific components such as phospholipase C.Laura CamarenaVincent BrunoGhia EuskirchenSebastian PoggioMichael SnyderPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 4, p e1000834 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Laura Camarena Vincent Bruno Ghia Euskirchen Sebastian Poggio Michael Snyder Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.
description	Acinetobacter baumannii is a common pathogen whose recent resistance to drugs has emerged as a major health problem. Ethanol has been found to increase the virulence of A. baumannii in Dictyostelium discoideum and Caenorhabditis elegans models of infection. To better understand the causes of this effect, we examined the transcriptional profile of A. baumannii grown in the presence or absence of ethanol using RNA-Seq. Using the Illumina/Solexa platform, a total of 43,453,960 reads (35 nt) were obtained, of which 3,596,474 mapped uniquely to the genome. Our analysis revealed that ethanol induces the expression of 49 genes that belong to different functional categories. A strong induction was observed for genes encoding metabolic enzymes, indicating that ethanol is efficiently assimilated. In addition, we detected the induction of genes encoding stress proteins, including upsA, hsp90, groEL and lon as well as permeases, efflux pumps and a secreted phospholipase C. In stationary phase, ethanol strongly induced several genes involved with iron assimilation and a high-affinity phosphate transport system, indicating that A. baumannii makes a better use of the iron and phosphate resources in the medium when ethanol is used as a carbon source. To evaluate the role of phospholipase C (Plc1) in virulence, we generated and analyzed a deletion mutant for plc1. This strain exhibits a modest, but reproducible, reduction in the cytotoxic effect caused by A. baumannii on epithelial cells, suggesting that phospholipase C is important for virulence. Overall, our results indicate the power of applying RNA-Seq to identify key modulators of bacterial pathogenesis. We suggest that the effect of ethanol on the virulence of A. baumannii is multifactorial and includes a general stress response and other specific components such as phospholipase C.
format	article
author	Laura Camarena Vincent Bruno Ghia Euskirchen Sebastian Poggio Michael Snyder
author_facet	Laura Camarena Vincent Bruno Ghia Euskirchen Sebastian Poggio Michael Snyder
author_sort	Laura Camarena
title	Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.
title_short	Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.
title_full	Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.
title_fullStr	Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.
title_full_unstemmed	Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.
title_sort	molecular mechanisms of ethanol-induced pathogenesis revealed by rna-sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/24f6110ba317460a91e99f402a51e5c9
work_keys_str_mv	AT lauracamarena molecularmechanismsofethanolinducedpathogenesisrevealedbyrnasequencing AT vincentbruno molecularmechanismsofethanolinducedpathogenesisrevealedbyrnasequencing AT ghiaeuskirchen molecularmechanismsofethanolinducedpathogenesisrevealedbyrnasequencing AT sebastianpoggio molecularmechanismsofethanolinducedpathogenesisrevealedbyrnasequencing AT michaelsnyder molecularmechanismsofethanolinducedpathogenesisrevealedbyrnasequencing
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Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing.

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