RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR

Abstract Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi g...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thais B. Rodrigues, Ramesh Kumar Dhandapani, Jian J. Duan, Subba Reddy Palli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24fd3910853f427c9f28f7ec502bc800
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:24fd3910853f427c9f28f7ec502bc800
record_format	dspace
spelling	oai:doaj.org-article:24fd3910853f427c9f28f7ec502bc8002021-12-02T11:52:30ZRNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR10.1038/s41598-017-08813-12045-2322https://doaj.org/article/24fd3910853f427c9f28f7ec502bc8002017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08813-1https://doaj.org/toc/2045-2322Abstract Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding proteins (dsRBP) as well as for proteins involved in dsRNA transport and the systemic RNAi. We also compared expression of six potential reference genes that could be used to normalize gene expression and selected gapdh and rpl32 as the most reliable genes among different tissues and stages of ALB. Injection of double-stranded RNA (dsRNA) targeting gene coding for inhibitor of apoptosis (IAP) into larvae and adults resulted in a significant knockdown of this gene and caused the death of 90% of the larvae and 100% of adults. No mortality of both larvae and adults injected with dsRNA targeting gene coding for green fluorescence protein (GFP, as a negative control) was observed. These data suggest that functional RNAi machinery exists in ALB and a potential RNAi-based method could be developed for controlling this insect.Thais B. RodriguesRamesh Kumar DhandapaniJian J. DuanSubba Reddy PalliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thais B. Rodrigues Ramesh Kumar Dhandapani Jian J. Duan Subba Reddy Palli RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
description	Abstract Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding proteins (dsRBP) as well as for proteins involved in dsRNA transport and the systemic RNAi. We also compared expression of six potential reference genes that could be used to normalize gene expression and selected gapdh and rpl32 as the most reliable genes among different tissues and stages of ALB. Injection of double-stranded RNA (dsRNA) targeting gene coding for inhibitor of apoptosis (IAP) into larvae and adults resulted in a significant knockdown of this gene and caused the death of 90% of the larvae and 100% of adults. No mortality of both larvae and adults injected with dsRNA targeting gene coding for green fluorescence protein (GFP, as a negative control) was observed. These data suggest that functional RNAi machinery exists in ALB and a potential RNAi-based method could be developed for controlling this insect.
format	article
author	Thais B. Rodrigues Ramesh Kumar Dhandapani Jian J. Duan Subba Reddy Palli
author_facet	Thais B. Rodrigues Ramesh Kumar Dhandapani Jian J. Duan Subba Reddy Palli
author_sort	Thais B. Rodrigues
title	RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
title_short	RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
title_full	RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
title_fullStr	RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
title_full_unstemmed	RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
title_sort	rna interference in the asian longhorned beetle:identification of key rnai genes and reference genes for rt-qpcr
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/24fd3910853f427c9f28f7ec502bc800
work_keys_str_mv	AT thaisbrodrigues rnainterferenceintheasianlonghornedbeetleidentificationofkeyrnaigenesandreferencegenesforrtqpcr AT rameshkumardhandapani rnainterferenceintheasianlonghornedbeetleidentificationofkeyrnaigenesandreferencegenesforrtqpcr AT jianjduan rnainterferenceintheasianlonghornedbeetleidentificationofkeyrnaigenesandreferencegenesforrtqpcr AT subbareddypalli rnainterferenceintheasianlonghornedbeetleidentificationofkeyrnaigenesandreferencegenesforrtqpcr
_version_	1718395035479703552

RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR

Ejemplares similares