Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour

The process of alveologenesis is incompletely understood, partly due to the lack of applicable real-time imaging methods. Here the authors describe the process of alveologenesis and the behaviour of epithelial cells in real-time, using widefield microscopy and image deconvolution in precision-cut lu...

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Autores principales: Khondoker M. Akram, Laura L. Yates, Róisín Mongey, Stephen Rothery, David C. A. Gaboriau, Jeremy Sanderson, Matthew Hind, Mark Griffiths, Charlotte H. Dean
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/24ffe26043ce43fea34a19872584d905
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Sumario:The process of alveologenesis is incompletely understood, partly due to the lack of applicable real-time imaging methods. Here the authors describe the process of alveologenesis and the behaviour of epithelial cells in real-time, using widefield microscopy and image deconvolution in precision-cut lung slices, revealing the dominant role of epithelial cell migration.