Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.

Bats harbor several highly pathogenic zoonotic viruses including Rabies, Marburg, and henipaviruses, without overt clinical symptoms in the animals. It has been suspected that bats might have evolved particularly effective mechanisms to suppress viral replication. Here, we investigated interferon (I...

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Autores principales:	Susanne E Biesold, Daniel Ritz, Florian Gloza-Rausch, Robert Wollny, Jan Felix Drexler, Victor M Corman, Elisabeth K V Kalko, Samuel Oppong, Christian Drosten, Marcel A Müller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/250bd677ca8b46089261210516cbe2c5
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spelling	oai:doaj.org-article:250bd677ca8b46089261210516cbe2c52021-11-18T07:33:21ZType I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.1932-620310.1371/journal.pone.0028131https://doaj.org/article/250bd677ca8b46089261210516cbe2c52011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22140523/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Bats harbor several highly pathogenic zoonotic viruses including Rabies, Marburg, and henipaviruses, without overt clinical symptoms in the animals. It has been suspected that bats might have evolved particularly effective mechanisms to suppress viral replication. Here, we investigated interferon (IFN) response, -induction, -secretion and -signaling in epithelial-like cells of the relevant and abundant African fruit bat species, Eidolon helvum (E. helvum). Immortalized cell lines were generated; their potential to induce and react on IFN was confirmed, and biological assays were adapted to application in bat cell cultures, enabling comparison of landmark IFN properties with that of common mammalian cell lines. E. helvum cells were fully capable of reacting to viral and artificial IFN stimuli. E. helvum cells showed highest IFN mRNA induction, highly productive IFN protein secretion, and evidence of efficient IFN stimulated gene induction. In an Alphavirus infection model, O'nyong-nyong virus exhibited strong IFN induction but evaded the IFN response by translational rather than transcriptional shutoff, similar to other Alphavirus infections. These novel IFN-competent cell lines will allow comparative research on zoonotic, bat-borne viruses in order to model mechanisms of viral maintenance and emergence in bat reservoirs.Susanne E BiesoldDaniel RitzFlorian Gloza-RauschRobert WollnyJan Felix DrexlerVictor M CormanElisabeth K V KalkoSamuel OppongChristian DrostenMarcel A MüllerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e28131 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susanne E Biesold Daniel Ritz Florian Gloza-Rausch Robert Wollny Jan Felix Drexler Victor M Corman Elisabeth K V Kalko Samuel Oppong Christian Drosten Marcel A Müller Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.
description	Bats harbor several highly pathogenic zoonotic viruses including Rabies, Marburg, and henipaviruses, without overt clinical symptoms in the animals. It has been suspected that bats might have evolved particularly effective mechanisms to suppress viral replication. Here, we investigated interferon (IFN) response, -induction, -secretion and -signaling in epithelial-like cells of the relevant and abundant African fruit bat species, Eidolon helvum (E. helvum). Immortalized cell lines were generated; their potential to induce and react on IFN was confirmed, and biological assays were adapted to application in bat cell cultures, enabling comparison of landmark IFN properties with that of common mammalian cell lines. E. helvum cells were fully capable of reacting to viral and artificial IFN stimuli. E. helvum cells showed highest IFN mRNA induction, highly productive IFN protein secretion, and evidence of efficient IFN stimulated gene induction. In an Alphavirus infection model, O'nyong-nyong virus exhibited strong IFN induction but evaded the IFN response by translational rather than transcriptional shutoff, similar to other Alphavirus infections. These novel IFN-competent cell lines will allow comparative research on zoonotic, bat-borne viruses in order to model mechanisms of viral maintenance and emergence in bat reservoirs.
format	article
author	Susanne E Biesold Daniel Ritz Florian Gloza-Rausch Robert Wollny Jan Felix Drexler Victor M Corman Elisabeth K V Kalko Samuel Oppong Christian Drosten Marcel A Müller
author_facet	Susanne E Biesold Daniel Ritz Florian Gloza-Rausch Robert Wollny Jan Felix Drexler Victor M Corman Elisabeth K V Kalko Samuel Oppong Christian Drosten Marcel A Müller
author_sort	Susanne E Biesold
title	Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.
title_short	Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.
title_full	Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.
title_fullStr	Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.
title_full_unstemmed	Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.
title_sort	type i interferon reaction to viral infection in interferon-competent, immortalized cell lines from the african fruit bat eidolon helvum.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/250bd677ca8b46089261210516cbe2c5
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Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.

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