KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST

BACKGROUND AND OBJECTIVE: Ki67 is proliferation marker in proliferative cells and P53 is a tumor suppressor gene. Meanwhile the cause of high recurrence of keratocyst is unknown. The purpose of this study was to evaluate immunostaining reaction of Ki67 (proliferation marker) and P53 (tumor suppresso...

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Autores principales:	S Seifi, S Mahjoub, E Shafigh, A Bijani, MH Ahangari
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2008
Materias:	ki67 protein p53 protein immunohistochemistry odontogenic keratocyst cell proliferation Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/250cd1774e5e4112a658633130b87fc2
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spelling	oai:doaj.org-article:250cd1774e5e4112a658633130b87fc22021-11-10T09:07:50ZKI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST1561-41072251-7170https://doaj.org/article/250cd1774e5e4112a658633130b87fc22008-12-01T00:00:00Zhttp://jbums.org/article-1-3097-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Ki67 is proliferation marker in proliferative cells and P53 is a tumor suppressor gene. Meanwhile the cause of high recurrence of keratocyst is unknown. The purpose of this study was to evaluate immunostaining reaction of Ki67 (proliferation marker) and P53 (tumor suppressor gene) in different and all layers of odontogenic keratocyst (OKC).METHODS: This descriptive study was performed on 20 paraffinized blocks. From parafinized blocks, 3-µm section was cutted and then IHC was done for Ki67, P53 markers with anti Ki67 antibody and anti P53 antibody. Nucleus of epithelial cells was immunostained in different layers of OKC in 1000 epithelial cells, in 10 HPF (high power field) were counted and compared to each other.FINDINGS: Immunostained nucleus of epithelial cells with Ki67 in basal, intermediate and surface layers and all lining epithelium of OKC were 14.3±4.4%, 41.4±12.8%, 0, 18.5±6.1%, respectively. Mean of nucleus immunoreactive epithelial cells with P53 in basal, intermediate, surface layers and all lining epithelium of OKC were 11.7±3.2%, 23.9±7.6%, 0, 11.8±3.6%, respectively. Mean of immunostained nucleus of epithelial cells with Ki67 and P53 markers was statistically significant in different layers of epithelium of OKC (p<0.0001). There was a significant relationship between mean of nucleus immunoreactive epithelial cells with Ki67 and P53 in intermediate layer of OKC. CONCLUSION: According to the results, high immunoreactivity with Ki67 and P53 markers in intermediate layers compared to basal, surface and in all lining epithelium of OKC showed exclusion of epithelial cells from normal cell cycle and tendency to recurrence and aggressive behavior in OKC. Also, high expression of Ki67 and P53 in intermediate layers could be used to differentiate aggressive of odontogenic cysts from other odontogenic cysts.S SeifiS MahjoubE Shafigh,A BijaniMH AhangariBabol University of Medical Sciencesarticleki67 proteinp53 proteinimmunohistochemistryodontogenic keratocystcell proliferationMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 10, Iss 5, Pp 23-29 (2008)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	ki67 protein p53 protein immunohistochemistry odontogenic keratocyst cell proliferation Medicine R Medicine (General) R5-920
spellingShingle	ki67 protein p53 protein immunohistochemistry odontogenic keratocyst cell proliferation Medicine R Medicine (General) R5-920 S Seifi S Mahjoub E Shafigh, A Bijani MH Ahangari KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
description	BACKGROUND AND OBJECTIVE: Ki67 is proliferation marker in proliferative cells and P53 is a tumor suppressor gene. Meanwhile the cause of high recurrence of keratocyst is unknown. The purpose of this study was to evaluate immunostaining reaction of Ki67 (proliferation marker) and P53 (tumor suppressor gene) in different and all layers of odontogenic keratocyst (OKC).METHODS: This descriptive study was performed on 20 paraffinized blocks. From parafinized blocks, 3-µm section was cutted and then IHC was done for Ki67, P53 markers with anti Ki67 antibody and anti P53 antibody. Nucleus of epithelial cells was immunostained in different layers of OKC in 1000 epithelial cells, in 10 HPF (high power field) were counted and compared to each other.FINDINGS: Immunostained nucleus of epithelial cells with Ki67 in basal, intermediate and surface layers and all lining epithelium of OKC were 14.3±4.4%, 41.4±12.8%, 0, 18.5±6.1%, respectively. Mean of nucleus immunoreactive epithelial cells with P53 in basal, intermediate, surface layers and all lining epithelium of OKC were 11.7±3.2%, 23.9±7.6%, 0, 11.8±3.6%, respectively. Mean of immunostained nucleus of epithelial cells with Ki67 and P53 markers was statistically significant in different layers of epithelium of OKC (p<0.0001). There was a significant relationship between mean of nucleus immunoreactive epithelial cells with Ki67 and P53 in intermediate layer of OKC. CONCLUSION: According to the results, high immunoreactivity with Ki67 and P53 markers in intermediate layers compared to basal, surface and in all lining epithelium of OKC showed exclusion of epithelial cells from normal cell cycle and tendency to recurrence and aggressive behavior in OKC. Also, high expression of Ki67 and P53 in intermediate layers could be used to differentiate aggressive of odontogenic cysts from other odontogenic cysts.
format	article
author	S Seifi S Mahjoub E Shafigh, A Bijani MH Ahangari
author_facet	S Seifi S Mahjoub E Shafigh, A Bijani MH Ahangari
author_sort	S Seifi
title	KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
title_short	KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
title_full	KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
title_fullStr	KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
title_full_unstemmed	KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
title_sort	ki67 and p53 proteins in odontogenic keratocyst
publisher	Babol University of Medical Sciences
publishDate	2008
url	https://doaj.org/article/250cd1774e5e4112a658633130b87fc2
work_keys_str_mv	AT sseifi ki67andp53proteinsinodontogenickeratocyst AT smahjoub ki67andp53proteinsinodontogenickeratocyst AT eshafigh ki67andp53proteinsinodontogenickeratocyst AT abijani ki67andp53proteinsinodontogenickeratocyst AT mhahangari ki67andp53proteinsinodontogenickeratocyst
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KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST

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