PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1

Abstract The epithelial–mesenchymal transition (EMT) is an embryonic program frequently reactivated during cancer progression and is implicated in cancer invasion and metastasis. Cancer cells can also acquire stem cell properties to self-renew and give rise to new tumors through the EMT. Inactivatio...

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Autores principales:	Yanmei Qi, Jie Liu, Joshua Chao, Mark P. Scheuerman, Saum A. Rahimi, Leonard Y. Lee, Shaohua Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/250e7653b49d4b5ab298c891dfc9a266
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spelling	oai:doaj.org-article:250e7653b49d4b5ab298c891dfc9a2662021-12-02T16:06:40ZPTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi110.1038/s41598-020-69698-12045-2322https://doaj.org/article/250e7653b49d4b5ab298c891dfc9a2662020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69698-1https://doaj.org/toc/2045-2322Abstract The epithelial–mesenchymal transition (EMT) is an embryonic program frequently reactivated during cancer progression and is implicated in cancer invasion and metastasis. Cancer cells can also acquire stem cell properties to self-renew and give rise to new tumors through the EMT. Inactivation of the tumor suppressor PTEN has been shown to induce the EMT, but the underlying molecular mechanisms are less understood. In this study, we reconstituted PTEN-deficient breast cancer cells with wild-type and mutant PTEN, demonstrating that restoration of PTEN expression converted cancer cells with mesenchymal traits to an epithelial phenotype and inhibited cancer stem cell (CSC) activity. The protein rather than the lipid phosphatase activity of PTEN accounts for the reversal of the EMT. PTEN dephosphorylates and downregulates Abi1 in breast cancer cells. Gain- and loss-of-function analysis indicates that upregulation of Abi1 mediates PTEN loss-induced EMT and CSC activity. These results suggest that PTEN may suppress breast cancer invasion and metastasis via dephosphorylating and downregulating Abi1.Yanmei QiJie LiuJoshua ChaoMark P. ScheuermanSaum A. RahimiLeonard Y. LeeShaohua LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanmei Qi Jie Liu Joshua Chao Mark P. Scheuerman Saum A. Rahimi Leonard Y. Lee Shaohua Li PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1
description	Abstract The epithelial–mesenchymal transition (EMT) is an embryonic program frequently reactivated during cancer progression and is implicated in cancer invasion and metastasis. Cancer cells can also acquire stem cell properties to self-renew and give rise to new tumors through the EMT. Inactivation of the tumor suppressor PTEN has been shown to induce the EMT, but the underlying molecular mechanisms are less understood. In this study, we reconstituted PTEN-deficient breast cancer cells with wild-type and mutant PTEN, demonstrating that restoration of PTEN expression converted cancer cells with mesenchymal traits to an epithelial phenotype and inhibited cancer stem cell (CSC) activity. The protein rather than the lipid phosphatase activity of PTEN accounts for the reversal of the EMT. PTEN dephosphorylates and downregulates Abi1 in breast cancer cells. Gain- and loss-of-function analysis indicates that upregulation of Abi1 mediates PTEN loss-induced EMT and CSC activity. These results suggest that PTEN may suppress breast cancer invasion and metastasis via dephosphorylating and downregulating Abi1.
format	article
author	Yanmei Qi Jie Liu Joshua Chao Mark P. Scheuerman Saum A. Rahimi Leonard Y. Lee Shaohua Li
author_facet	Yanmei Qi Jie Liu Joshua Chao Mark P. Scheuerman Saum A. Rahimi Leonard Y. Lee Shaohua Li
author_sort	Yanmei Qi
title	PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1
title_short	PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1
title_full	PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1
title_fullStr	PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1
title_full_unstemmed	PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1
title_sort	pten suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating abi1
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/250e7653b49d4b5ab298c891dfc9a266
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_version_	1718384891469496320

PTEN suppresses epithelial–mesenchymal transition and cancer stem cell activity by downregulating Abi1

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