Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan

Abstract Mammalian orthoreovirus (MRV), a non-enveloped virus with a ten-segmented double-stranded RNA genome, infects virtually all mammals, including humans. Human infection with MRV seems to be common in early childhood, but is rarely symptomatic. Despite the ubiquitous presence of MRV in mammals...

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Autores principales:	Kouichi Kitamura, Hirotaka Takagi, Tomoichiro Oka, Michiyo Kataoka, Yo Ueki, Akie Sakagami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/250ef6c681ab4ec1bf0e261b63c3270c
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spelling	oai:doaj.org-article:250ef6c681ab4ec1bf0e261b63c3270c2021-12-02T17:41:32ZIntertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan10.1038/s41598-021-92019-z2045-2322https://doaj.org/article/250ef6c681ab4ec1bf0e261b63c3270c2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92019-zhttps://doaj.org/toc/2045-2322Abstract Mammalian orthoreovirus (MRV), a non-enveloped virus with a ten-segmented double-stranded RNA genome, infects virtually all mammals, including humans. Human infection with MRV seems to be common in early childhood, but is rarely symptomatic. Despite the ubiquitous presence of MRV in mammals as well as in environmental waters, the molecular characterisation of the MRV genome remains to be fully elucidated. In this study, two novel strains, MRV-2 THK0325 and MRV-1 THK0617, were unintentionally isolated from wastewater in Japan via an environmental surveillance of enteric viruses. Homology and phylogenetic analysis demonstrated that all the segments of THK0325 were closely related to the MRV-2 Osaka strains, which were recently proposed to have existed for at least two decades in Japan. Most of the segments in THK0617 also showed a close relationship with the MRV-2 Osaka strains, but the M2, S1, and S3 segments belong to another MRV cluster. According to the S1 sequence, the determinant of serotype THK0617 was classified as MRV-1, and both the M2 and S3 segments were closely related to MRV-1 and -3 from the tree shrew in China. These results suggest that the MRV-2 Osaka-like strain spread widely throughout Japan, accompanied by intertypic reassortment occurring in East Asia.Kouichi KitamuraHirotaka TakagiTomoichiro OkaMichiyo KataokaYo UekiAkie SakagamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kouichi Kitamura Hirotaka Takagi Tomoichiro Oka Michiyo Kataoka Yo Ueki Akie Sakagami Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan
description	Abstract Mammalian orthoreovirus (MRV), a non-enveloped virus with a ten-segmented double-stranded RNA genome, infects virtually all mammals, including humans. Human infection with MRV seems to be common in early childhood, but is rarely symptomatic. Despite the ubiquitous presence of MRV in mammals as well as in environmental waters, the molecular characterisation of the MRV genome remains to be fully elucidated. In this study, two novel strains, MRV-2 THK0325 and MRV-1 THK0617, were unintentionally isolated from wastewater in Japan via an environmental surveillance of enteric viruses. Homology and phylogenetic analysis demonstrated that all the segments of THK0325 were closely related to the MRV-2 Osaka strains, which were recently proposed to have existed for at least two decades in Japan. Most of the segments in THK0617 also showed a close relationship with the MRV-2 Osaka strains, but the M2, S1, and S3 segments belong to another MRV cluster. According to the S1 sequence, the determinant of serotype THK0617 was classified as MRV-1, and both the M2 and S3 segments were closely related to MRV-1 and -3 from the tree shrew in China. These results suggest that the MRV-2 Osaka-like strain spread widely throughout Japan, accompanied by intertypic reassortment occurring in East Asia.
format	article
author	Kouichi Kitamura Hirotaka Takagi Tomoichiro Oka Michiyo Kataoka Yo Ueki Akie Sakagami
author_facet	Kouichi Kitamura Hirotaka Takagi Tomoichiro Oka Michiyo Kataoka Yo Ueki Akie Sakagami
author_sort	Kouichi Kitamura
title	Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan
title_short	Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan
title_full	Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan
title_fullStr	Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan
title_full_unstemmed	Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan
title_sort	intertypic reassortment of mammalian orthoreovirus identified in wastewater in japan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/250ef6c681ab4ec1bf0e261b63c3270c
work_keys_str_mv	AT kouichikitamura intertypicreassortmentofmammalianorthoreovirusidentifiedinwastewaterinjapan AT hirotakatakagi intertypicreassortmentofmammalianorthoreovirusidentifiedinwastewaterinjapan AT tomoichirooka intertypicreassortmentofmammalianorthoreovirusidentifiedinwastewaterinjapan AT michiyokataoka intertypicreassortmentofmammalianorthoreovirusidentifiedinwastewaterinjapan AT youeki intertypicreassortmentofmammalianorthoreovirusidentifiedinwastewaterinjapan AT akiesakagami intertypicreassortmentofmammalianorthoreovirusidentifiedinwastewaterinjapan
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Intertypic reassortment of mammalian orthoreovirus identified in wastewater in Japan

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