Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha

Abstract According to the investigation of wild Actinidia eriantha in Jiangxi province of China, we found that soluble solids content of fruit was lower than edible standard (14%). However, we found a high-sugar type A. eriantha line (code was ‘MM24’, test material) during investigative process at N...

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Autores principales:	Xiaobiao Xu, Guanglian Liao, Chunhui Huang, Min Zhong, Dongfeng Jia, Xueyan Qu, Qing Liu, Yanqun He, Yiqi Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/251b601feae642a3b3123ca9d068ca92
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spelling	oai:doaj.org-article:251b601feae642a3b3123ca9d068ca922021-12-02T15:10:19ZDifferences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha10.1038/s41598-020-77464-62045-2322https://doaj.org/article/251b601feae642a3b3123ca9d068ca922020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77464-6https://doaj.org/toc/2045-2322Abstract According to the investigation of wild Actinidia eriantha in Jiangxi province of China, we found that soluble solids content of fruit was lower than edible standard (14%). However, we found a high-sugar type A. eriantha line (code was ‘MM24’, test material) during investigative process at Nancheng county (E 116° 48′, N 26° 23′, 845 m). We sheared its scions to asexual reproduction in Fengxin County (rootstock was A. deliciosa ‘Miliang 1’ with 7 years old) and at the same time DUS (Distinctness, Uniformity and Stability) test was also carried out. There were uncontested differences between the two comparative genotypes according to the results of polyacrylamide gel electrophoresis, it can be judged as a new cultivar. In addition, there was great similarity on most important morphological and quality characteristics. While, there was difference on SSC, DM and TS between the two materials on ripen fruit, these indicators were much higher on test material than on control. The sugar degree assessment showed that the sugar degree of test material was strong and retention time was long. Further, no sucrose was found before DAF 135 d in test material and sucrose were significantly higher than in control only at DAF 165 d and DAF 175 d. The qRT-PCR results of sucrose-related genes showed that the relative expression levels of AcSPS1, AcSPS3, AcSPS5 and AcSUS5 genes were consistent with the sucrose accumulation trend, which was probably the main genes for the difference in sugar degree.Xiaobiao XuGuanglian LiaoChunhui HuangMin ZhongDongfeng JiaXueyan QuQing LiuYanqun HeYiqi LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaobiao Xu Guanglian Liao Chunhui Huang Min Zhong Dongfeng Jia Xueyan Qu Qing Liu Yanqun He Yiqi Li Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha
description	Abstract According to the investigation of wild Actinidia eriantha in Jiangxi province of China, we found that soluble solids content of fruit was lower than edible standard (14%). However, we found a high-sugar type A. eriantha line (code was ‘MM24’, test material) during investigative process at Nancheng county (E 116° 48′, N 26° 23′, 845 m). We sheared its scions to asexual reproduction in Fengxin County (rootstock was A. deliciosa ‘Miliang 1’ with 7 years old) and at the same time DUS (Distinctness, Uniformity and Stability) test was also carried out. There were uncontested differences between the two comparative genotypes according to the results of polyacrylamide gel electrophoresis, it can be judged as a new cultivar. In addition, there was great similarity on most important morphological and quality characteristics. While, there was difference on SSC, DM and TS between the two materials on ripen fruit, these indicators were much higher on test material than on control. The sugar degree assessment showed that the sugar degree of test material was strong and retention time was long. Further, no sucrose was found before DAF 135 d in test material and sucrose were significantly higher than in control only at DAF 165 d and DAF 175 d. The qRT-PCR results of sucrose-related genes showed that the relative expression levels of AcSPS1, AcSPS3, AcSPS5 and AcSUS5 genes were consistent with the sucrose accumulation trend, which was probably the main genes for the difference in sugar degree.
format	article
author	Xiaobiao Xu Guanglian Liao Chunhui Huang Min Zhong Dongfeng Jia Xueyan Qu Qing Liu Yanqun He Yiqi Li
author_facet	Xiaobiao Xu Guanglian Liao Chunhui Huang Min Zhong Dongfeng Jia Xueyan Qu Qing Liu Yanqun He Yiqi Li
author_sort	Xiaobiao Xu
title	Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha
title_short	Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha
title_full	Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha
title_fullStr	Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha
title_full_unstemmed	Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha
title_sort	differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of actinidia eriantha
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/251b601feae642a3b3123ca9d068ca92
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Differences of sucrose accumulation concentration and related genes expression between two sucrose accumulation types of Actinidia eriantha

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