Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model

Abstract Axonal motor driven cargo utilizes the microtubule cytoskeleton in order to direct cargo, such as synaptic vesicle precursors (SVP), to where they are needed. This transport requires vesicles to travel up to microns in distance. It has recently been observed that finite microtubule lengths...

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Autores principales:	M. W. Gramlich, S. Balseiro-Gómez, S. M. Ali Tabei, M. Parkes, S. Yogev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/251c2e5ce23f4893a55e84759e2426a8
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spelling	oai:doaj.org-article:251c2e5ce23f4893a55e84759e2426a82021-12-02T14:26:54ZDistinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model10.1038/s41598-021-82836-72045-2322https://doaj.org/article/251c2e5ce23f4893a55e84759e2426a82021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82836-7https://doaj.org/toc/2045-2322Abstract Axonal motor driven cargo utilizes the microtubule cytoskeleton in order to direct cargo, such as synaptic vesicle precursors (SVP), to where they are needed. This transport requires vesicles to travel up to microns in distance. It has recently been observed that finite microtubule lengths can act as roadblocks inhibiting SVP and increasing the time required for transport. SVPs reach the end of a microtubule and pause until they can navigate to a neighboring microtubule in order to continue transport. The mechanism(s) by which axonal SVPs navigate the end of a microtubule in order to continue mobility is unknown. In this manuscript we model experimentally observed vesicle pausing at microtubule ends in C. elegans. We show that a single rate-constant model reproduces the time SVPs pause at MT-ends. This model is based on the time an SVP must detach from its current microtubule and re-attach to a neighboring microtubule. We show that vesicle pause times are different for anterograde and retrograde motion, suggesting that vesicles utilize different proteins at plus and minus end sites. Last, we show that vesicles do not likely utilize a tug-of-war like mechanism and reverse direction in order to navigate microtubule ends.M. W. GramlichS. Balseiro-GómezS. M. Ali TabeiM. ParkesS. YogevNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. W. Gramlich S. Balseiro-Gómez S. M. Ali Tabei M. Parkes S. Yogev Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
description	Abstract Axonal motor driven cargo utilizes the microtubule cytoskeleton in order to direct cargo, such as synaptic vesicle precursors (SVP), to where they are needed. This transport requires vesicles to travel up to microns in distance. It has recently been observed that finite microtubule lengths can act as roadblocks inhibiting SVP and increasing the time required for transport. SVPs reach the end of a microtubule and pause until they can navigate to a neighboring microtubule in order to continue transport. The mechanism(s) by which axonal SVPs navigate the end of a microtubule in order to continue mobility is unknown. In this manuscript we model experimentally observed vesicle pausing at microtubule ends in C. elegans. We show that a single rate-constant model reproduces the time SVPs pause at MT-ends. This model is based on the time an SVP must detach from its current microtubule and re-attach to a neighboring microtubule. We show that vesicle pause times are different for anterograde and retrograde motion, suggesting that vesicles utilize different proteins at plus and minus end sites. Last, we show that vesicles do not likely utilize a tug-of-war like mechanism and reverse direction in order to navigate microtubule ends.
format	article
author	M. W. Gramlich S. Balseiro-Gómez S. M. Ali Tabei M. Parkes S. Yogev
author_facet	M. W. Gramlich S. Balseiro-Gómez S. M. Ali Tabei M. Parkes S. Yogev
author_sort	M. W. Gramlich
title	Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
title_short	Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
title_full	Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
title_fullStr	Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
title_full_unstemmed	Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
title_sort	distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/251c2e5ce23f4893a55e84759e2426a8
work_keys_str_mv	AT mwgramlich distinguishingsynapticvesicleprecursornavigationofmicrotubuleendswithasinglerateconstantmodel AT sbalseirogomez distinguishingsynapticvesicleprecursornavigationofmicrotubuleendswithasinglerateconstantmodel AT smalitabei distinguishingsynapticvesicleprecursornavigationofmicrotubuleendswithasinglerateconstantmodel AT mparkes distinguishingsynapticvesicleprecursornavigationofmicrotubuleendswithasinglerateconstantmodel AT syogev distinguishingsynapticvesicleprecursornavigationofmicrotubuleendswithasinglerateconstantmodel
_version_	1718391297798045696

Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model

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