MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity

Ting Zhang,1,2 Meng Tang,1,2 Ying Yao,1 Ying Ma,1 Yuepu Pu1,2 1Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Occupational and Environmental Health, School of Public Health & Collaborative Innovation Center of Suzhou Nano Science and Technolog...

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Autores principales:	Zhang T, Tang M, Yao Y, Ma Y, Pu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	multiwalled carbon nanotubes surface-functionalized protein corona cellular uptake cytotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/251f0b86817e4b7f8b87e347dada5bcd
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spelling	oai:doaj.org-article:251f0b86817e4b7f8b87e347dada5bcd2021-12-02T07:38:42ZMWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity1178-2013https://doaj.org/article/251f0b86817e4b7f8b87e347dada5bcd2019-02-01T00:00:00Zhttps://www.dovepress.com/mwcnt-interactions-with-protein-surface-induced-changes-in-protein-ads-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ting Zhang,1,2 Meng Tang,1,2 Ying Yao,1 Ying Ma,1 Yuepu Pu1,2 1Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Occupational and Environmental Health, School of Public Health & Collaborative Innovation Center of Suzhou Nano Science and Technology Southeast University, Nanjing, Jiangsu, 210009, China; 2Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, Jiangsu, 210009, China Purpose: Protein adsorption onto nanoparticles in the form of protein corona, affects properties of nanomaterials and their behavior in the biological milieu. This study aims at exploring the effects of multiwalled carbon nanotubes (MWCNTs) surface chemistry on bovine serum albumin (BSA) and immunoglobulin G (IgG), including their adsorption behavior and spatial configurations, as well as the impact on cellular uptake, cytotoxicity, and cellular responses. Methods: Three types of MWCNTs (pristine MWCNTs, MWCNTs-COOH, and MWCNTs-PEG) were synthesized by classical chemical reduction. The size, morphology, hydrodynamic size, and zeta potential were characterized using transmission electron microscopy and dynamic light scattering. MWCNTs were exposed to BSA and IgG solutions, then the amount of MWCNT absorption was performed by bicinchoninic acid assay, and the effects were assessed by utilizing fluorescence spectroscopy, circular dichroism (CD) spectroscopy. Quantitative measurement of MWCNTs uptake with or without protein corona was performed as turbidity method. CCK assay and a microdilution method were performed to evaluate the effects of protein corona on cytotoxicity and pro-inflammatory cytokines release.Results: The BSA and IgG adsorption capacities of MWCNTs followed the order pristine MWCNTs>MWCNTs-COOH and MWCNTs-PEG. MWCNT binding can cause fluorescence quenching and conformational changes in BSA and IgG, indicating that both the physicochemical properties of MWCNTs and protein properties play critical roles in determining their adsorption behavior. Further study showed time-dependent increases in MWCNT cellular uptake and internalization. Hydrophobicity is the major factor increasing cellular uptake of pristine MWCNTs, but a protein corona enriched with dysoposnins is the main factor reducing uptake of MWCNT-COOH by RAW264.7 cells. The cytotoxicity and pro-inflammatory response related to physicochemical properties of MWCNTs, and frustrated phagocytosis is a key initiating event in the pro-inflammatory response of MWCNT-exposed macrophages. Conclusion: These findings shed light on how functionalized MWCNTs interact with protein coronas and provide useful insight into the dramatic effect of protein coronas on different functionalized MWCNTs. These events affect cellular uptake and cytotoxicity, which could inform how to enhance MWCNT biocompatibility and develop approaches for managing MWCNT hazards. Keywords: multiwalled carbon nanotubes, protein corona, cellular uptake, cytotoxicity, inflammation  Zhang TTang MYao YMa YPu YDove Medical Pressarticlemultiwalled carbon nanotubessurface-functionalizedprotein coronacellular uptakecytotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 993-1009 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	multiwalled carbon nanotubes surface-functionalized protein corona cellular uptake cytotoxicity Medicine (General) R5-920
spellingShingle	multiwalled carbon nanotubes surface-functionalized protein corona cellular uptake cytotoxicity Medicine (General) R5-920 Zhang T Tang M Yao Y Ma Y Pu Y MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
description	Ting Zhang,1,2 Meng Tang,1,2 Ying Yao,1 Ying Ma,1 Yuepu Pu1,2 1Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Occupational and Environmental Health, School of Public Health & Collaborative Innovation Center of Suzhou Nano Science and Technology Southeast University, Nanjing, Jiangsu, 210009, China; 2Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, Jiangsu, 210009, China Purpose: Protein adsorption onto nanoparticles in the form of protein corona, affects properties of nanomaterials and their behavior in the biological milieu. This study aims at exploring the effects of multiwalled carbon nanotubes (MWCNTs) surface chemistry on bovine serum albumin (BSA) and immunoglobulin G (IgG), including their adsorption behavior and spatial configurations, as well as the impact on cellular uptake, cytotoxicity, and cellular responses. Methods: Three types of MWCNTs (pristine MWCNTs, MWCNTs-COOH, and MWCNTs-PEG) were synthesized by classical chemical reduction. The size, morphology, hydrodynamic size, and zeta potential were characterized using transmission electron microscopy and dynamic light scattering. MWCNTs were exposed to BSA and IgG solutions, then the amount of MWCNT absorption was performed by bicinchoninic acid assay, and the effects were assessed by utilizing fluorescence spectroscopy, circular dichroism (CD) spectroscopy. Quantitative measurement of MWCNTs uptake with or without protein corona was performed as turbidity method. CCK assay and a microdilution method were performed to evaluate the effects of protein corona on cytotoxicity and pro-inflammatory cytokines release.Results: The BSA and IgG adsorption capacities of MWCNTs followed the order pristine MWCNTs>MWCNTs-COOH and MWCNTs-PEG. MWCNT binding can cause fluorescence quenching and conformational changes in BSA and IgG, indicating that both the physicochemical properties of MWCNTs and protein properties play critical roles in determining their adsorption behavior. Further study showed time-dependent increases in MWCNT cellular uptake and internalization. Hydrophobicity is the major factor increasing cellular uptake of pristine MWCNTs, but a protein corona enriched with dysoposnins is the main factor reducing uptake of MWCNT-COOH by RAW264.7 cells. The cytotoxicity and pro-inflammatory response related to physicochemical properties of MWCNTs, and frustrated phagocytosis is a key initiating event in the pro-inflammatory response of MWCNT-exposed macrophages. Conclusion: These findings shed light on how functionalized MWCNTs interact with protein coronas and provide useful insight into the dramatic effect of protein coronas on different functionalized MWCNTs. These events affect cellular uptake and cytotoxicity, which could inform how to enhance MWCNT biocompatibility and develop approaches for managing MWCNT hazards. Keywords: multiwalled carbon nanotubes, protein corona, cellular uptake, cytotoxicity, inflammation
format	article
author	Zhang T Tang M Yao Y Ma Y Pu Y
author_facet	Zhang T Tang M Yao Y Ma Y Pu Y
author_sort	Zhang T
title	MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
title_short	MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
title_full	MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
title_fullStr	MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
title_full_unstemmed	MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
title_sort	mwcnt interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/251f0b86817e4b7f8b87e347dada5bcd
work_keys_str_mv	AT zhangt mwcntinteractionswithproteinsurfaceinducedchangesinproteinadsorptionandtheimpactofproteincoronaoncellularuptakeandcytotoxicity AT tangm mwcntinteractionswithproteinsurfaceinducedchangesinproteinadsorptionandtheimpactofproteincoronaoncellularuptakeandcytotoxicity AT yaoy mwcntinteractionswithproteinsurfaceinducedchangesinproteinadsorptionandtheimpactofproteincoronaoncellularuptakeandcytotoxicity AT may mwcntinteractionswithproteinsurfaceinducedchangesinproteinadsorptionandtheimpactofproteincoronaoncellularuptakeandcytotoxicity AT puy mwcntinteractionswithproteinsurfaceinducedchangesinproteinadsorptionandtheimpactofproteincoronaoncellularuptakeandcytotoxicity
_version_	1718399269471256576

MWCNT interactions with protein: surface-induced changes in protein adsorption and the impact of protein corona on cellular uptake and cytotoxicity

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