C/EBPδ gene targets in human keratinocytes.

C/EBPs are a family of B-Zip transcription factors--TFs--involved in the regulation of differentiation in several tissues. The two most studied members--C/EBPα and C/EBPβ--play important roles in skin homeostasis and their ablation reveals cells with stem cells signatures. Much less is known about C...

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Autores principales:	Serena Borrelli, Daniele Fanoni, Diletta Dolfini, Daniela Alotto, Maria Ravo, Olì Maria Victoria Grober, Alessandro Weisz, Carlotta Castagnoli, Emilio Berti, M Alessandra Vigano, Roberto Mantovani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2520ed40875f4147af2a2fe8c38a66c6
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spelling	oai:doaj.org-article:2520ed40875f4147af2a2fe8c38a66c62021-11-18T07:02:35ZC/EBPδ gene targets in human keratinocytes.1932-620310.1371/journal.pone.0013789https://doaj.org/article/2520ed40875f4147af2a2fe8c38a66c62010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21072181/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203C/EBPs are a family of B-Zip transcription factors--TFs--involved in the regulation of differentiation in several tissues. The two most studied members--C/EBPα and C/EBPβ--play important roles in skin homeostasis and their ablation reveals cells with stem cells signatures. Much less is known about C/EBPδ which is highly expressed in the granular layer of interfollicular epidermis and is a direct target of p63, the master regular of multilayered epithelia. We identified C/EBPδ target genes in human primary keratinocytes by ChIP on chip and profiling of cells functionally inactivated with siRNA. Categorization suggests a role in differentiation and control of cell-cycle, particularly of G2/M genes. Among positively controlled targets are numerous genes involved in barrier function. Functional inactivation of C/EBPδ as well as overexpressions of two TF targets--MafB and SOX2--affect expression of markers of keratinocyte differentiation. We performed IHC on skin tumor tissue arrays: expression of C/EBPδ is lost in Basal Cell Carcinomas, but a majority of Squamous Cell Carcinomas showed elevated levels of the protein. Our data indicate that C/EBPδ plays a role in late stages of keratinocyte differentiation.Serena BorrelliDaniele FanoniDiletta DolfiniDaniela AlottoMaria RavoOlì Maria Victoria GroberAlessandro WeiszCarlotta CastagnoliEmilio BertiM Alessandra ViganoRoberto MantovaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 11, p e13789 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Serena Borrelli Daniele Fanoni Diletta Dolfini Daniela Alotto Maria Ravo Olì Maria Victoria Grober Alessandro Weisz Carlotta Castagnoli Emilio Berti M Alessandra Vigano Roberto Mantovani C/EBPδ gene targets in human keratinocytes.
description	C/EBPs are a family of B-Zip transcription factors--TFs--involved in the regulation of differentiation in several tissues. The two most studied members--C/EBPα and C/EBPβ--play important roles in skin homeostasis and their ablation reveals cells with stem cells signatures. Much less is known about C/EBPδ which is highly expressed in the granular layer of interfollicular epidermis and is a direct target of p63, the master regular of multilayered epithelia. We identified C/EBPδ target genes in human primary keratinocytes by ChIP on chip and profiling of cells functionally inactivated with siRNA. Categorization suggests a role in differentiation and control of cell-cycle, particularly of G2/M genes. Among positively controlled targets are numerous genes involved in barrier function. Functional inactivation of C/EBPδ as well as overexpressions of two TF targets--MafB and SOX2--affect expression of markers of keratinocyte differentiation. We performed IHC on skin tumor tissue arrays: expression of C/EBPδ is lost in Basal Cell Carcinomas, but a majority of Squamous Cell Carcinomas showed elevated levels of the protein. Our data indicate that C/EBPδ plays a role in late stages of keratinocyte differentiation.
format	article
author	Serena Borrelli Daniele Fanoni Diletta Dolfini Daniela Alotto Maria Ravo Olì Maria Victoria Grober Alessandro Weisz Carlotta Castagnoli Emilio Berti M Alessandra Vigano Roberto Mantovani
author_facet	Serena Borrelli Daniele Fanoni Diletta Dolfini Daniela Alotto Maria Ravo Olì Maria Victoria Grober Alessandro Weisz Carlotta Castagnoli Emilio Berti M Alessandra Vigano Roberto Mantovani
author_sort	Serena Borrelli
title	C/EBPδ gene targets in human keratinocytes.
title_short	C/EBPδ gene targets in human keratinocytes.
title_full	C/EBPδ gene targets in human keratinocytes.
title_fullStr	C/EBPδ gene targets in human keratinocytes.
title_full_unstemmed	C/EBPδ gene targets in human keratinocytes.
title_sort	c/ebpδ gene targets in human keratinocytes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/2520ed40875f4147af2a2fe8c38a66c6
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C/EBPδ gene targets in human keratinocytes.

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