Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens

Prodigiosin is a ‘natural red pigment produced by Serratia marcescens which exhibits immunosuppressive and anticancer properties in addition to antimicrobial activities. This work presents an attempt to maximize the production of prodigiosin by two different strategies: one factor at time (OFAT) an...

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Autores principales: Sura Jasem Mohammed, Khalid Jaber Kadhum, Khalid waleed Hameed
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Publicado: Al-Khwarizmi College of Engineering – University of Baghdad 2018
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Acceso en línea:https://doaj.org/article/25366ebce1e2459ca5e43e180427c929
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spelling oai:doaj.org-article:25366ebce1e2459ca5e43e180427c9292021-12-02T05:46:59ZClassical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens10.22153/kej.2018.03.0061818-11712312-0789https://doaj.org/article/25366ebce1e2459ca5e43e180427c9292018-12-01T00:00:00Zhttp://alkej.uobaghdad.edu.iq/index.php/alkej/article/view/62https://doaj.org/toc/1818-1171https://doaj.org/toc/2312-0789 Prodigiosin is a ‘natural red pigment produced by Serratia marcescens which exhibits immunosuppressive and anticancer properties in addition to antimicrobial activities. This work presents an attempt to maximize the production of prodigiosin by two different strategies: one factor at time (OFAT) and statistical optimization. The result of OFAT revealed that sucrose and peptone were the best carbon and nitrogen sources for pigment production with concentration of prodigiosin of about 135 mg/ L. This value was increased to 331.6mg/ L with an optimized ratio of C/N (60:40) and reached 356.8 with pH 6 and 2% inoculum size at end of classical optimization. Statistical experimental design based on Response surface methodology was conducted to optimize the composition of trace element. The design revealed that the predicted prodigiosin production of 406 mg/L can be achieved when concentrations of trace element CaCl2·2H2O, FeSO4·4H2O, MgSO4·7H2O and MnSO4·4H2O were equal to 9.22, 0.32, 0.67 and 2.48 g/L, respectively. The actual production of prodigiosin in the optimized medium was 375  mg/L. Growth kinetics of S. marcescens were evaluated in optimized medium which revealed that prodigiosin was ‘non-associated growth’ secondary metabolite with maximum production of approximately 365.7 mg/L obtained after 54 hours of incubation.   Sura Jasem MohammedKhalid Jaber KadhumKhalid waleed HameedAl-Khwarizmi College of Engineering – University of BaghdadarticleClassical optimization, prodigiosin, Serratia marcescens , statistical optimizationChemical engineeringTP155-156Engineering (General). Civil engineering (General)TA1-2040ENAl-Khawarizmi Engineering Journal, Vol 14, Iss 4 (2018)
institution DOAJ
collection DOAJ
language EN
topic Classical optimization, prodigiosin, Serratia marcescens , statistical optimization
Chemical engineering
TP155-156
Engineering (General). Civil engineering (General)
TA1-2040
spellingShingle Classical optimization, prodigiosin, Serratia marcescens , statistical optimization
Chemical engineering
TP155-156
Engineering (General). Civil engineering (General)
TA1-2040
Sura Jasem Mohammed
Khalid Jaber Kadhum
Khalid waleed Hameed
Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens
description Prodigiosin is a ‘natural red pigment produced by Serratia marcescens which exhibits immunosuppressive and anticancer properties in addition to antimicrobial activities. This work presents an attempt to maximize the production of prodigiosin by two different strategies: one factor at time (OFAT) and statistical optimization. The result of OFAT revealed that sucrose and peptone were the best carbon and nitrogen sources for pigment production with concentration of prodigiosin of about 135 mg/ L. This value was increased to 331.6mg/ L with an optimized ratio of C/N (60:40) and reached 356.8 with pH 6 and 2% inoculum size at end of classical optimization. Statistical experimental design based on Response surface methodology was conducted to optimize the composition of trace element. The design revealed that the predicted prodigiosin production of 406 mg/L can be achieved when concentrations of trace element CaCl2·2H2O, FeSO4·4H2O, MgSO4·7H2O and MnSO4·4H2O were equal to 9.22, 0.32, 0.67 and 2.48 g/L, respectively. The actual production of prodigiosin in the optimized medium was 375  mg/L. Growth kinetics of S. marcescens were evaluated in optimized medium which revealed that prodigiosin was ‘non-associated growth’ secondary metabolite with maximum production of approximately 365.7 mg/L obtained after 54 hours of incubation.  
format article
author Sura Jasem Mohammed
Khalid Jaber Kadhum
Khalid waleed Hameed
author_facet Sura Jasem Mohammed
Khalid Jaber Kadhum
Khalid waleed Hameed
author_sort Sura Jasem Mohammed
title Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens
title_short Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens
title_full Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens
title_fullStr Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens
title_full_unstemmed Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens
title_sort classical and statistical optimization of medium composition for promoting prodigiosin produced by local isolate of serratia marcescens
publisher Al-Khwarizmi College of Engineering – University of Baghdad
publishDate 2018
url https://doaj.org/article/25366ebce1e2459ca5e43e180427c929
work_keys_str_mv AT surajasemmohammed classicalandstatisticaloptimizationofmediumcompositionforpromotingprodigiosinproducedbylocalisolateofserratiamarcescens
AT khalidjaberkadhum classicalandstatisticaloptimizationofmediumcompositionforpromotingprodigiosinproducedbylocalisolateofserratiamarcescens
AT khalidwaleedhameed classicalandstatisticaloptimizationofmediumcompositionforpromotingprodigiosinproducedbylocalisolateofserratiamarcescens
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