Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation

Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation

Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has facilitated dramatic progress in the field of genome engineering. Whilst microinjection of the Cas9 protein and a single guide RNA (sgRNA) into mouse zygotes is a widespread method for producing genetical...

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Autores principales: Satoru Iwata, Hitomi Nakadai, Daisuke Fukushi, Mami Jose, Miki Nagahara, Takashi Iwamoto
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/253e25a047044934a4003b6259c44dd2
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