Essential oil-loaded lipid nanoparticles for wound healing

Francesca Saporito,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Silvia Rossi,1 Cinzia Boselli,1 Antonia Icaro Cornaglia,2 Barbara Mannucci,3 Pietro Grisoli,1 Barbara Vigani,1 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, 2Department of Public Health, Experimental and...

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Autores principales:	Saporito F, Sandri G, Bonferoni MC, Rossi S, Boselli C, Icaro Cornaglia A, Mannucci B, Grisoli P, Vigani B, Ferrari F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	lipid nanoparticles eucalyptus essential oil antimicrobial properties in vitro and in vivo wound healing properties Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/253e63349a5c47c0856e3d80a833ffe6
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spelling	oai:doaj.org-article:253e63349a5c47c0856e3d80a833ffe62021-12-02T08:35:51ZEssential oil-loaded lipid nanoparticles for wound healing1178-2013https://doaj.org/article/253e63349a5c47c0856e3d80a833ffe62017-12-01T00:00:00Zhttps://www.dovepress.com/essential-oil-loaded-lipid-nanoparticles-for-wound-healing-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Francesca Saporito,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Silvia Rossi,1 Cinzia Boselli,1 Antonia Icaro Cornaglia,2 Barbara Mannucci,3 Pietro Grisoli,1 Barbara Vigani,1 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, 2Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, 3Centro Grandi Strumenti, University of Pavia, Pavia, Italy Abstract: Chronic wounds and severe burns are diseases responsible for severe morbidity and even death. Wound repair is a crucial process and tissue regeneration enhancement and infection prevention are key factors to minimize pain, discomfort, and scar formation. The aim of this work was the development of lipid nanoparticles (solid lipid nanoparticles and nanostructured lipid carriers [NLC]), to be loaded with eucalyptus or rosemary essential oils and to be used, as medical devices, to enhance healing of skin wounds. Lipid nanoparticles were based on natural lipids: cocoa butter, as solid lipid, and olive oil or sesame oil, as liquid lipids. Lecithin was chosen as surfactant to stabilize nanoparticles and to prevent their aggregation. The systems were prepared by high shear homogenization followed by ultrasound application. Nanoparticles were characterized for physical–chemical properties, bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward normal human dermal fibroblasts. Antimicrobial activity of nanoparticles was evaluated against two reference microbial strains, one of Staphylococcus aureus, the other of Streptococcus pyogenes. Finally, the capability of nanoparticles to promote wound healing in vivo was evaluated on a rat burn model. NLC based on olive oil and loaded with eucalyptus oil showed appropriate physical–chemical properties, good bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward fibroblasts, associated to antimicrobial properties. Moreover, the in vivo results evidenced the capability of these NLC to enhance the healing process. Olive oil, which is characterized by a high content of oleic acid, proved to exert a synergic effect with eucalyptus oil with respect to antimicrobial activity and wound repair promotion. Keywords: lipid nanoparticles, eucalyptus essential oil, antimicrobial properties, wound healingSaporito FSandri GBonferoni MCRossi SBoselli CIcaro Cornaglia AMannucci BGrisoli PVigani BFerrari FDove Medical Pressarticlelipid nanoparticleseucalyptus essential oilantimicrobial propertiesin vitro and in vivo wound healing propertiesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 175-186 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid nanoparticles eucalyptus essential oil antimicrobial properties in vitro and in vivo wound healing properties Medicine (General) R5-920
spellingShingle	lipid nanoparticles eucalyptus essential oil antimicrobial properties in vitro and in vivo wound healing properties Medicine (General) R5-920 Saporito F Sandri G Bonferoni MC Rossi S Boselli C Icaro Cornaglia A Mannucci B Grisoli P Vigani B Ferrari F Essential oil-loaded lipid nanoparticles for wound healing
description	Francesca Saporito,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Silvia Rossi,1 Cinzia Boselli,1 Antonia Icaro Cornaglia,2 Barbara Mannucci,3 Pietro Grisoli,1 Barbara Vigani,1 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, 2Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, 3Centro Grandi Strumenti, University of Pavia, Pavia, Italy Abstract: Chronic wounds and severe burns are diseases responsible for severe morbidity and even death. Wound repair is a crucial process and tissue regeneration enhancement and infection prevention are key factors to minimize pain, discomfort, and scar formation. The aim of this work was the development of lipid nanoparticles (solid lipid nanoparticles and nanostructured lipid carriers [NLC]), to be loaded with eucalyptus or rosemary essential oils and to be used, as medical devices, to enhance healing of skin wounds. Lipid nanoparticles were based on natural lipids: cocoa butter, as solid lipid, and olive oil or sesame oil, as liquid lipids. Lecithin was chosen as surfactant to stabilize nanoparticles and to prevent their aggregation. The systems were prepared by high shear homogenization followed by ultrasound application. Nanoparticles were characterized for physical–chemical properties, bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward normal human dermal fibroblasts. Antimicrobial activity of nanoparticles was evaluated against two reference microbial strains, one of Staphylococcus aureus, the other of Streptococcus pyogenes. Finally, the capability of nanoparticles to promote wound healing in vivo was evaluated on a rat burn model. NLC based on olive oil and loaded with eucalyptus oil showed appropriate physical–chemical properties, good bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward fibroblasts, associated to antimicrobial properties. Moreover, the in vivo results evidenced the capability of these NLC to enhance the healing process. Olive oil, which is characterized by a high content of oleic acid, proved to exert a synergic effect with eucalyptus oil with respect to antimicrobial activity and wound repair promotion. Keywords: lipid nanoparticles, eucalyptus essential oil, antimicrobial properties, wound healing
format	article
author	Saporito F Sandri G Bonferoni MC Rossi S Boselli C Icaro Cornaglia A Mannucci B Grisoli P Vigani B Ferrari F
author_facet	Saporito F Sandri G Bonferoni MC Rossi S Boselli C Icaro Cornaglia A Mannucci B Grisoli P Vigani B Ferrari F
author_sort	Saporito F
title	Essential oil-loaded lipid nanoparticles for wound healing
title_short	Essential oil-loaded lipid nanoparticles for wound healing
title_full	Essential oil-loaded lipid nanoparticles for wound healing
title_fullStr	Essential oil-loaded lipid nanoparticles for wound healing
title_full_unstemmed	Essential oil-loaded lipid nanoparticles for wound healing
title_sort	essential oil-loaded lipid nanoparticles for wound healing
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/253e63349a5c47c0856e3d80a833ffe6
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Essential oil-loaded lipid nanoparticles for wound healing

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