Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells

Single-molecule localization microscopy (SMLM) requires the use of fluorophores with specific sets of properties. Here the authors employ conventional BODIPY dyes as SMLM fluorophores by making use of rarely reported red-shifted ground state BODIPY dimers to image fatty acids, lipid droplets and lys...

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Autores principales:	Santosh Adhikari, Joe Moscatelli, Elizabeth M. Smith, Chiranjib Banerjee, Elias M. Puchner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2540317227af4dadb8f1c4f456ee4df3
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spelling	oai:doaj.org-article:2540317227af4dadb8f1c4f456ee4df32021-12-02T14:40:20ZSingle-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells10.1038/s41467-019-11384-62041-1723https://doaj.org/article/2540317227af4dadb8f1c4f456ee4df32019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11384-6https://doaj.org/toc/2041-1723Single-molecule localization microscopy (SMLM) requires the use of fluorophores with specific sets of properties. Here the authors employ conventional BODIPY dyes as SMLM fluorophores by making use of rarely reported red-shifted ground state BODIPY dimers to image fatty acids, lipid droplets and lysosomes at single-molecule resolution.Santosh AdhikariJoe MoscatelliElizabeth M. SmithChiranjib BanerjeeElias M. PuchnerNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Santosh Adhikari Joe Moscatelli Elizabeth M. Smith Chiranjib Banerjee Elias M. Puchner Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
description	Single-molecule localization microscopy (SMLM) requires the use of fluorophores with specific sets of properties. Here the authors employ conventional BODIPY dyes as SMLM fluorophores by making use of rarely reported red-shifted ground state BODIPY dimers to image fatty acids, lipid droplets and lysosomes at single-molecule resolution.
format	article
author	Santosh Adhikari Joe Moscatelli Elizabeth M. Smith Chiranjib Banerjee Elias M. Puchner
author_facet	Santosh Adhikari Joe Moscatelli Elizabeth M. Smith Chiranjib Banerjee Elias M. Puchner
author_sort	Santosh Adhikari
title	Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
title_short	Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
title_full	Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
title_fullStr	Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
title_full_unstemmed	Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
title_sort	single-molecule localization microscopy and tracking with red-shifted states of conventional bodipy conjugates in living cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/2540317227af4dadb8f1c4f456ee4df3
work_keys_str_mv	AT santoshadhikari singlemoleculelocalizationmicroscopyandtrackingwithredshiftedstatesofconventionalbodipyconjugatesinlivingcells AT joemoscatelli singlemoleculelocalizationmicroscopyandtrackingwithredshiftedstatesofconventionalbodipyconjugatesinlivingcells AT elizabethmsmith singlemoleculelocalizationmicroscopyandtrackingwithredshiftedstatesofconventionalbodipyconjugatesinlivingcells AT chiranjibbanerjee singlemoleculelocalizationmicroscopyandtrackingwithredshiftedstatesofconventionalbodipyconjugatesinlivingcells AT eliasmpuchner singlemoleculelocalizationmicroscopyandtrackingwithredshiftedstatesofconventionalbodipyconjugatesinlivingcells
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Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells

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