Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum

Abstract Several Lactobacillus plantarum strains are marketed as probiotics for their potential health benefits. Prebiotics, e.g., galacto-oligosaccharides (GOS), have the potential to selectively stimulate the growth of L. plantarum probiotic strains based on their phenotypic diversity in carbohydr...

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Autores principales:	Jori Fuhren, Markus Schwalbe, Lucía Peralta-Marzal, Christiane Rösch, Henk A. Schols, Michiel Kleerebezem
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2541e0d6953e4d4f912ff5fd48d4ffe7
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spelling	oai:doaj.org-article:2541e0d6953e4d4f912ff5fd48d4ffe72021-12-02T16:18:02ZPhenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum10.1038/s41598-020-78721-42045-2322https://doaj.org/article/2541e0d6953e4d4f912ff5fd48d4ffe72020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78721-4https://doaj.org/toc/2045-2322Abstract Several Lactobacillus plantarum strains are marketed as probiotics for their potential health benefits. Prebiotics, e.g., galacto-oligosaccharides (GOS), have the potential to selectively stimulate the growth of L. plantarum probiotic strains based on their phenotypic diversity in carbohydrate utilization, and thereby enhance their health promoting effects in the host in a strain-specific manner. Previously, we have shown that GOS variably promotes the strain-specific growth of L. plantarum. In this study we investigated this variation by molecular analysis of GOS utilization by L. plantarum. HPAEC-PAD analysis revealed two distinct GOS utilization phenotypes in L. plantarum. Linking these phenotypes to the strain-specific genotypes led to the identification of a lac operon encoding a β-galactosidase (lacA), a permease (lacS), and a divergently oriented regulator (lacR), that are predicted to be involved in the utilization of higher degree of polymerization (DP) constituents present in GOS (specifically DP of 3–4). Mutation of lacA and lacS in L. plantarum NC8 resulted in reduced growth on GOS, and HPAEC analysis confirmed the role of these genes in the import and utilization of higher-DP GOS constituents. Overall, the results enable the design of highly-selective synbiotic combinations of L. plantarum strain-specific probiotics and specific GOS-prebiotic fractions.Jori FuhrenMarkus SchwalbeLucía Peralta-MarzalChristiane RöschHenk A. ScholsMichiel KleerebezemNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jori Fuhren Markus Schwalbe Lucía Peralta-Marzal Christiane Rösch Henk A. Schols Michiel Kleerebezem Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
description	Abstract Several Lactobacillus plantarum strains are marketed as probiotics for their potential health benefits. Prebiotics, e.g., galacto-oligosaccharides (GOS), have the potential to selectively stimulate the growth of L. plantarum probiotic strains based on their phenotypic diversity in carbohydrate utilization, and thereby enhance their health promoting effects in the host in a strain-specific manner. Previously, we have shown that GOS variably promotes the strain-specific growth of L. plantarum. In this study we investigated this variation by molecular analysis of GOS utilization by L. plantarum. HPAEC-PAD analysis revealed two distinct GOS utilization phenotypes in L. plantarum. Linking these phenotypes to the strain-specific genotypes led to the identification of a lac operon encoding a β-galactosidase (lacA), a permease (lacS), and a divergently oriented regulator (lacR), that are predicted to be involved in the utilization of higher degree of polymerization (DP) constituents present in GOS (specifically DP of 3–4). Mutation of lacA and lacS in L. plantarum NC8 resulted in reduced growth on GOS, and HPAEC analysis confirmed the role of these genes in the import and utilization of higher-DP GOS constituents. Overall, the results enable the design of highly-selective synbiotic combinations of L. plantarum strain-specific probiotics and specific GOS-prebiotic fractions.
format	article
author	Jori Fuhren Markus Schwalbe Lucía Peralta-Marzal Christiane Rösch Henk A. Schols Michiel Kleerebezem
author_facet	Jori Fuhren Markus Schwalbe Lucía Peralta-Marzal Christiane Rösch Henk A. Schols Michiel Kleerebezem
author_sort	Jori Fuhren
title	Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
title_short	Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
title_full	Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
title_fullStr	Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
title_full_unstemmed	Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
title_sort	phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in lactobacillus plantarum
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2541e0d6953e4d4f912ff5fd48d4ffe7
work_keys_str_mv	AT jorifuhren phenotypicandgeneticcharacterizationofdifferentialgalactooligosaccharideutilizationinlactobacillusplantarum AT markusschwalbe phenotypicandgeneticcharacterizationofdifferentialgalactooligosaccharideutilizationinlactobacillusplantarum AT luciaperaltamarzal phenotypicandgeneticcharacterizationofdifferentialgalactooligosaccharideutilizationinlactobacillusplantarum AT christianerosch phenotypicandgeneticcharacterizationofdifferentialgalactooligosaccharideutilizationinlactobacillusplantarum AT henkaschols phenotypicandgeneticcharacterizationofdifferentialgalactooligosaccharideutilizationinlactobacillusplantarum AT michielkleerebezem phenotypicandgeneticcharacterizationofdifferentialgalactooligosaccharideutilizationinlactobacillusplantarum
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Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum

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