LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis

Abstract LINE-1 hypomethylation is widely accepted as marker for global genomic DNA hypomethylation, which is a frequent event in cancer. The aim of the study was to evaluate LINE-1 methylation status at different stages of gastric carcinogenesis and evaluate its prognostic potential in clinical set...

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Autores principales:	Juozas Kupcinskas, Ruta Steponaitiene, Cosima Langner, Giedre Smailyte, Jurgita Skieceviciene, Limas Kupcinskas, Peter Malfertheiner, Alexander Link
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2562d8b937c24df4857df6864b7c47f9
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spelling	oai:doaj.org-article:2562d8b937c24df4857df6864b7c47f92021-12-02T15:05:38ZLINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis10.1038/s41598-017-05143-02045-2322https://doaj.org/article/2562d8b937c24df4857df6864b7c47f92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05143-0https://doaj.org/toc/2045-2322Abstract LINE-1 hypomethylation is widely accepted as marker for global genomic DNA hypomethylation, which is a frequent event in cancer. The aim of the study was to evaluate LINE-1 methylation status at different stages of gastric carcinogenesis and evaluate its prognostic potential in clinical settings. LINE-1 methylation was analyzed in 267 tissue samples by bisulfite pyrosequencing including primary colorectal cancer tissues (T-CRC) with corresponding adjacent colon mucosa (N-CRC), gastric cancer tissues (T-GC) with corresponding gastric mucosa (N-GC), normal gastric tissues (N), chronic non-atrophic and atrophic gastritis (CG). LINE-1 methylation level was lower in both T-GC and T-CRC when compared to paired adjacent tissues. No difference was observed for LINE-1 methylation status between patients with normal gastric mucosa, CG and N-GC. LINE-1 methylation in T-GC but not N-GC tended to correlate with age. Subgroup stratification analysis did not reveal significant differences in LINE-1 methylation status according to tumor stage, anatomical location, histological subtype, differentiation grade. We observed similar overall survival data between patients with high or low LINE-1 levels. In summary, LINE-1 hypomethylation is a characteristic feature in GC but not very common in early preneoplastic stages of gastric carcinogenesis. Prognostic role of LINE-1 hypomethylation in GC patients could not be confirmed in this cohort.Juozas KupcinskasRuta SteponaitieneCosima LangnerGiedre SmailyteJurgita SkiecevicieneLimas KupcinskasPeter MalfertheinerAlexander LinkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juozas Kupcinskas Ruta Steponaitiene Cosima Langner Giedre Smailyte Jurgita Skieceviciene Limas Kupcinskas Peter Malfertheiner Alexander Link LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
description	Abstract LINE-1 hypomethylation is widely accepted as marker for global genomic DNA hypomethylation, which is a frequent event in cancer. The aim of the study was to evaluate LINE-1 methylation status at different stages of gastric carcinogenesis and evaluate its prognostic potential in clinical settings. LINE-1 methylation was analyzed in 267 tissue samples by bisulfite pyrosequencing including primary colorectal cancer tissues (T-CRC) with corresponding adjacent colon mucosa (N-CRC), gastric cancer tissues (T-GC) with corresponding gastric mucosa (N-GC), normal gastric tissues (N), chronic non-atrophic and atrophic gastritis (CG). LINE-1 methylation level was lower in both T-GC and T-CRC when compared to paired adjacent tissues. No difference was observed for LINE-1 methylation status between patients with normal gastric mucosa, CG and N-GC. LINE-1 methylation in T-GC but not N-GC tended to correlate with age. Subgroup stratification analysis did not reveal significant differences in LINE-1 methylation status according to tumor stage, anatomical location, histological subtype, differentiation grade. We observed similar overall survival data between patients with high or low LINE-1 levels. In summary, LINE-1 hypomethylation is a characteristic feature in GC but not very common in early preneoplastic stages of gastric carcinogenesis. Prognostic role of LINE-1 hypomethylation in GC patients could not be confirmed in this cohort.
format	article
author	Juozas Kupcinskas Ruta Steponaitiene Cosima Langner Giedre Smailyte Jurgita Skieceviciene Limas Kupcinskas Peter Malfertheiner Alexander Link
author_facet	Juozas Kupcinskas Ruta Steponaitiene Cosima Langner Giedre Smailyte Jurgita Skieceviciene Limas Kupcinskas Peter Malfertheiner Alexander Link
author_sort	Juozas Kupcinskas
title	LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
title_short	LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
title_full	LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
title_fullStr	LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
title_full_unstemmed	LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
title_sort	line-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2562d8b937c24df4857df6864b7c47f9
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LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis

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