HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium

ABSTRACT Bacterial cell division, with a few exceptions, is driven by FtsZ through a treadmilling mechanism to remodel and constrict the rigid peptidoglycan (PG) layer. Yet different organisms may differ in the composition of the cell division complex (divisome). In the filamentous cyanobacterium An...

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Autores principales:	Wei-Yue Xing, Jing Liu, Zi-Qian Wang, Ju-Yuan Zhang, Xiaoli Zeng, Yiling Yang, Cheng-Cai Zhang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	cell division FtsZ heterocysts cyanobacteria peptidoglycan Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/25697b3f61d34d36a7c904da06b53561
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spelling	oai:doaj.org-article:25697b3f61d34d36a7c904da06b535612021-11-10T18:37:51ZHetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium10.1128/mBio.01382-212150-7511https://doaj.org/article/25697b3f61d34d36a7c904da06b535612021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01382-21https://doaj.org/toc/2150-7511ABSTRACT Bacterial cell division, with a few exceptions, is driven by FtsZ through a treadmilling mechanism to remodel and constrict the rigid peptidoglycan (PG) layer. Yet different organisms may differ in the composition of the cell division complex (divisome). In the filamentous cyanobacterium Anabaena sp. strain PCC 7120, hetF is required for the initiation of the differentiation of heterocysts, cells specialized in N2 fixation under combined-nitrogen deprivation. In this study, we demonstrate that hetF is expressed in vegetative cells and necessary for cell division under certain conditions. Under nonpermissive conditions, cells of a ΔhetF mutant stop dividing, consistent with increased levels of HetF under similar conditions in the wild type. Furthermore, HetF is a membrane protein located at midcell and cell-cell junctions. In the absence of HetF, FtsZ rings are still present in the elongated cells; however, PG remodeling is abolished. This phenotype is similar to that observed with the inhibition of the septal PG synthase FtsI. We further reveal that HetF is recruited to or stabilized at the divisome by interacting with FtsI and that this interaction is necessary for HetF function in cell division. Our results indicate that HetF is a member of the divisome depending mainly on light intensity and reveal distinct features of the cell division machinery in cyanobacteria that are of high ecological and environmental importance. IMPORTANCE Cyanobacteria shaped the Earth’s evolutionary history and are still playing important roles for elementary cycles in different environments. They consist of highly diverse species with different cell shapes, sizes, and morphologies. Although these properties are strongly affected by the process of cytokinesis, the mechanism remains largely unexplored. Using different approaches, we demonstrate that HetF is a new component of the cell division machinery under certain environmental conditions in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. The common and diverged characteristics of cell division in prokaryotes reflect the evolutionary history of different bacteria as an adaptive measure to proliferate under certain environmental conditions. As a protein for cell differentiation, the recruitment of HetF to the septum illustrates such an adaptive mechanism in cyanobacteria.Wei-Yue XingJing LiuZi-Qian WangJu-Yuan ZhangXiaoli ZengYiling YangCheng-Cai ZhangAmerican Society for Microbiologyarticlecell divisionFtsZheterocystscyanobacteriapeptidoglycanMicrobiologyQR1-502ENmBio, Vol 12, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell division FtsZ heterocysts cyanobacteria peptidoglycan Microbiology QR1-502
spellingShingle	cell division FtsZ heterocysts cyanobacteria peptidoglycan Microbiology QR1-502 Wei-Yue Xing Jing Liu Zi-Qian Wang Ju-Yuan Zhang Xiaoli Zeng Yiling Yang Cheng-Cai Zhang HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium
description	ABSTRACT Bacterial cell division, with a few exceptions, is driven by FtsZ through a treadmilling mechanism to remodel and constrict the rigid peptidoglycan (PG) layer. Yet different organisms may differ in the composition of the cell division complex (divisome). In the filamentous cyanobacterium Anabaena sp. strain PCC 7120, hetF is required for the initiation of the differentiation of heterocysts, cells specialized in N2 fixation under combined-nitrogen deprivation. In this study, we demonstrate that hetF is expressed in vegetative cells and necessary for cell division under certain conditions. Under nonpermissive conditions, cells of a ΔhetF mutant stop dividing, consistent with increased levels of HetF under similar conditions in the wild type. Furthermore, HetF is a membrane protein located at midcell and cell-cell junctions. In the absence of HetF, FtsZ rings are still present in the elongated cells; however, PG remodeling is abolished. This phenotype is similar to that observed with the inhibition of the septal PG synthase FtsI. We further reveal that HetF is recruited to or stabilized at the divisome by interacting with FtsI and that this interaction is necessary for HetF function in cell division. Our results indicate that HetF is a member of the divisome depending mainly on light intensity and reveal distinct features of the cell division machinery in cyanobacteria that are of high ecological and environmental importance. IMPORTANCE Cyanobacteria shaped the Earth’s evolutionary history and are still playing important roles for elementary cycles in different environments. They consist of highly diverse species with different cell shapes, sizes, and morphologies. Although these properties are strongly affected by the process of cytokinesis, the mechanism remains largely unexplored. Using different approaches, we demonstrate that HetF is a new component of the cell division machinery under certain environmental conditions in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. The common and diverged characteristics of cell division in prokaryotes reflect the evolutionary history of different bacteria as an adaptive measure to proliferate under certain environmental conditions. As a protein for cell differentiation, the recruitment of HetF to the septum illustrates such an adaptive mechanism in cyanobacteria.
format	article
author	Wei-Yue Xing Jing Liu Zi-Qian Wang Ju-Yuan Zhang Xiaoli Zeng Yiling Yang Cheng-Cai Zhang
author_facet	Wei-Yue Xing Jing Liu Zi-Qian Wang Ju-Yuan Zhang Xiaoli Zeng Yiling Yang Cheng-Cai Zhang
author_sort	Wei-Yue Xing
title	HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium
title_short	HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium
title_full	HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium
title_fullStr	HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium
title_full_unstemmed	HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium
title_sort	hetf protein is a new divisome component in a filamentous and developmental cyanobacterium
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/25697b3f61d34d36a7c904da06b53561
work_keys_str_mv	AT weiyuexing hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium AT jingliu hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium AT ziqianwang hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium AT juyuanzhang hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium AT xiaolizeng hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium AT yilingyang hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium AT chengcaizhang hetfproteinisanewdivisomecomponentinafilamentousanddevelopmentalcyanobacterium
_version_	1718439758940602368

HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium

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