Identification of microRNAs as potential prognostic markers in ependymoma.

<h4>Introduction</h4>We have examined expression of microRNAs (miRNAs) in ependymomas to identify molecular markers of value for clinical management. miRNAs are non-coding RNAs that can block mRNA translation and affect mRNA stability. Changes in the expression of miRNAs have been correl...

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Autores principales:	Fabricio F Costa, Jared M Bischof, Elio F Vanin, Rishi R Lulla, Min Wang, Simone T Sredni, Veena Rajaram, Maria de Fátima Bonaldo, Deli Wang, Stewart Goldman, Tadanori Tomita, Marcelo B Soares
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2569c213f1f540f2bf59aa946e6a4937
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spelling	oai:doaj.org-article:2569c213f1f540f2bf59aa946e6a49372021-11-18T07:35:30ZIdentification of microRNAs as potential prognostic markers in ependymoma.1932-620310.1371/journal.pone.0025114https://doaj.org/article/2569c213f1f540f2bf59aa946e6a49372011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22053178/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>We have examined expression of microRNAs (miRNAs) in ependymomas to identify molecular markers of value for clinical management. miRNAs are non-coding RNAs that can block mRNA translation and affect mRNA stability. Changes in the expression of miRNAs have been correlated with many human cancers.<h4>Materials and methods</h4>We have utilized TaqMan Low Density Arrays to evaluate the expression of 365 miRNAs in ependymomas and normal brain tissue. We first demonstrated the similarity of expression profiles of paired frozen tissue (FT) and paraffin-embedded specimens (FFPE). We compared the miRNA expression profiles of 34 FFPE ependymoma samples with 8 microdissected normal brain tissue specimens enriched for ependymal cells. miRNA expression profiles were then correlated with tumor location, histology and other clinicopathological features.<h4>Results</h4>We have identified miRNAs that are over-expressed in ependymomas, such as miR-135a and miR-17-5p, and down-regulated, such as miR-383 and miR-485-5p. We have also uncovered associations between expression of specific miRNAs which portend a worse prognosis. For example, we have identified a cluster of miRNAs on human chromosome 14q32 that is associated with time to relapse. We also found that miR-203 is an independent marker for relapse compared to the parameters that are currently used. Additionally, we have identified three miRNAs (let-7d, miR-596 and miR-367) that strongly correlate to overall survival.<h4>Conclusion</h4>We have identified miRNAs that are differentially expressed in ependymomas compared with normal ependymal tissue. We have also uncovered significant associations of miRNAs with clinical behavior. This is the first report of clinically relevant miRNAs in ependymomas.Fabricio F CostaJared M BischofElio F VaninRishi R LullaMin WangSimone T SredniVeena RajaramMaria de Fátima BonaldoDeli WangStewart GoldmanTadanori TomitaMarcelo B SoaresPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e25114 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabricio F Costa Jared M Bischof Elio F Vanin Rishi R Lulla Min Wang Simone T Sredni Veena Rajaram Maria de Fátima Bonaldo Deli Wang Stewart Goldman Tadanori Tomita Marcelo B Soares Identification of microRNAs as potential prognostic markers in ependymoma.
description	<h4>Introduction</h4>We have examined expression of microRNAs (miRNAs) in ependymomas to identify molecular markers of value for clinical management. miRNAs are non-coding RNAs that can block mRNA translation and affect mRNA stability. Changes in the expression of miRNAs have been correlated with many human cancers.<h4>Materials and methods</h4>We have utilized TaqMan Low Density Arrays to evaluate the expression of 365 miRNAs in ependymomas and normal brain tissue. We first demonstrated the similarity of expression profiles of paired frozen tissue (FT) and paraffin-embedded specimens (FFPE). We compared the miRNA expression profiles of 34 FFPE ependymoma samples with 8 microdissected normal brain tissue specimens enriched for ependymal cells. miRNA expression profiles were then correlated with tumor location, histology and other clinicopathological features.<h4>Results</h4>We have identified miRNAs that are over-expressed in ependymomas, such as miR-135a and miR-17-5p, and down-regulated, such as miR-383 and miR-485-5p. We have also uncovered associations between expression of specific miRNAs which portend a worse prognosis. For example, we have identified a cluster of miRNAs on human chromosome 14q32 that is associated with time to relapse. We also found that miR-203 is an independent marker for relapse compared to the parameters that are currently used. Additionally, we have identified three miRNAs (let-7d, miR-596 and miR-367) that strongly correlate to overall survival.<h4>Conclusion</h4>We have identified miRNAs that are differentially expressed in ependymomas compared with normal ependymal tissue. We have also uncovered significant associations of miRNAs with clinical behavior. This is the first report of clinically relevant miRNAs in ependymomas.
format	article
author	Fabricio F Costa Jared M Bischof Elio F Vanin Rishi R Lulla Min Wang Simone T Sredni Veena Rajaram Maria de Fátima Bonaldo Deli Wang Stewart Goldman Tadanori Tomita Marcelo B Soares
author_facet	Fabricio F Costa Jared M Bischof Elio F Vanin Rishi R Lulla Min Wang Simone T Sredni Veena Rajaram Maria de Fátima Bonaldo Deli Wang Stewart Goldman Tadanori Tomita Marcelo B Soares
author_sort	Fabricio F Costa
title	Identification of microRNAs as potential prognostic markers in ependymoma.
title_short	Identification of microRNAs as potential prognostic markers in ependymoma.
title_full	Identification of microRNAs as potential prognostic markers in ependymoma.
title_fullStr	Identification of microRNAs as potential prognostic markers in ependymoma.
title_full_unstemmed	Identification of microRNAs as potential prognostic markers in ependymoma.
title_sort	identification of micrornas as potential prognostic markers in ependymoma.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2569c213f1f540f2bf59aa946e6a4937
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Identification of microRNAs as potential prognostic markers in ependymoma.

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