Functionalized titanium implant in regulating bacteria and cell response

Jianfeng Jin,1 Dongdong Fei,1 Yumei Zhang,2 Qintao Wang1 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of S...

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Autores principales:	Jin J, Fei D, Zhang Y, Wang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Functionalized titanium implant Ti-GO-Ag nanocomposite Surface topography Material characterization Antibacterial mechanism Cell behavior Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/256a79c0402d47169eb9780f069c7faa
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spelling	oai:doaj.org-article:256a79c0402d47169eb9780f069c7faa2021-12-02T02:14:04ZFunctionalized titanium implant in regulating bacteria and cell response1178-2013https://doaj.org/article/256a79c0402d47169eb9780f069c7faa2019-02-01T00:00:00Zhttps://www.dovepress.com/functionalized-titanium-implant-in-regulating-bacteria-and-cell-respon-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jianfeng Jin,1 Dongdong Fei,1 Yumei Zhang,2 Qintao Wang1 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China; 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China Background: Biological complications are an issue of critical interest in contemporary dental and orthopedic fields. Although titanium (Ti), graphene oxide (GO) or silver (Ag) particles are suitable for biomedical implants due to their excellent cytocompatibility, bioactivity, and antibacterial properties, the exact antibacterial mechanism is not understood when the three substances are combined (Ti-GO-Ag). Materials and methods: In this work, the material characterization, antibacterial property, antibacterial mechanisms, and cell behavior of Ti-GO-Ag fabricated by electroplating and ultraviolet reduction methods respectively, were investigated in detail. Results: The material characterization of Ti-GO-Ag tested by atomic force microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, nanoindentation, nanoscratch, inductively coupled plasma mass spectrometer, and contact angle tester revealed the importance of GO concentration and Ag content in the preparation process. The antibacterial tests of Ti-GO-Ag clearly demonstrated the whole process of bacteria interacting with materials, including reactive oxygen species, endocytosis, aggregation, perforation, and leakage. In addition, the behavior of Ti-GO-Ag showed that cell area, length, width, and fluorescence intensity were affected. Conclusion: Briefly, Ti-GO-Ag nanocomposite was a dual-functionalized implant biomaterial with antibacterial and biocompatible characterization. Keywords: functionalized titanium implant, Ti-GO-Ag nanocomposite, surface topography, material characterization, antibacterial mechanism, cell behaviorJin JFei DZhang YWang QDove Medical PressarticleFunctionalized titanium implantTi-GO-Ag nanocompositeSurface topographyMaterial characterizationAntibacterial mechanismCell behaviorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1433-1450 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Functionalized titanium implant Ti-GO-Ag nanocomposite Surface topography Material characterization Antibacterial mechanism Cell behavior Medicine (General) R5-920
spellingShingle	Functionalized titanium implant Ti-GO-Ag nanocomposite Surface topography Material characterization Antibacterial mechanism Cell behavior Medicine (General) R5-920 Jin J Fei D Zhang Y Wang Q Functionalized titanium implant in regulating bacteria and cell response
description	Jianfeng Jin,1 Dongdong Fei,1 Yumei Zhang,2 Qintao Wang1 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China; 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China Background: Biological complications are an issue of critical interest in contemporary dental and orthopedic fields. Although titanium (Ti), graphene oxide (GO) or silver (Ag) particles are suitable for biomedical implants due to their excellent cytocompatibility, bioactivity, and antibacterial properties, the exact antibacterial mechanism is not understood when the three substances are combined (Ti-GO-Ag). Materials and methods: In this work, the material characterization, antibacterial property, antibacterial mechanisms, and cell behavior of Ti-GO-Ag fabricated by electroplating and ultraviolet reduction methods respectively, were investigated in detail. Results: The material characterization of Ti-GO-Ag tested by atomic force microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, nanoindentation, nanoscratch, inductively coupled plasma mass spectrometer, and contact angle tester revealed the importance of GO concentration and Ag content in the preparation process. The antibacterial tests of Ti-GO-Ag clearly demonstrated the whole process of bacteria interacting with materials, including reactive oxygen species, endocytosis, aggregation, perforation, and leakage. In addition, the behavior of Ti-GO-Ag showed that cell area, length, width, and fluorescence intensity were affected. Conclusion: Briefly, Ti-GO-Ag nanocomposite was a dual-functionalized implant biomaterial with antibacterial and biocompatible characterization. Keywords: functionalized titanium implant, Ti-GO-Ag nanocomposite, surface topography, material characterization, antibacterial mechanism, cell behavior
format	article
author	Jin J Fei D Zhang Y Wang Q
author_facet	Jin J Fei D Zhang Y Wang Q
author_sort	Jin J
title	Functionalized titanium implant in regulating bacteria and cell response
title_short	Functionalized titanium implant in regulating bacteria and cell response
title_full	Functionalized titanium implant in regulating bacteria and cell response
title_fullStr	Functionalized titanium implant in regulating bacteria and cell response
title_full_unstemmed	Functionalized titanium implant in regulating bacteria and cell response
title_sort	functionalized titanium implant in regulating bacteria and cell response
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/256a79c0402d47169eb9780f069c7faa
work_keys_str_mv	AT jinj functionalizedtitaniumimplantinregulatingbacteriaandcellresponse AT feid functionalizedtitaniumimplantinregulatingbacteriaandcellresponse AT zhangy functionalizedtitaniumimplantinregulatingbacteriaandcellresponse AT wangq functionalizedtitaniumimplantinregulatingbacteriaandcellresponse
_version_	1718402580330053632

Functionalized titanium implant in regulating bacteria and cell response

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