Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from Opuntia stricta var. Dilenii’s Fruits: Optimization and Biological Activities

Opuntia stricta var. Dillenii’s prickly pears are an underutilized fruit with a high content of betalains and phenolic compounds that could bring potential health benefits for humans. The aim of this study is the optimization of the “green” extraction of betalai...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Iván Gómez-López, Gloria Lobo-Rodrigo, María P. Portillo, M. Pilar Cano
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Opuntia stricta</i> var. <i>Dillenii</i> whole fruits ultrasound-assisted extraction green solvents betalains phenolic compounds antioxidant activity Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/256eed5f4dd44478b6bdb307b629535b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:256eed5f4dd44478b6bdb307b629535b
record_format	dspace
spelling	oai:doaj.org-article:256eed5f4dd44478b6bdb307b629535b2021-11-25T16:28:35ZUltrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities10.3390/antiox101117862076-3921https://doaj.org/article/256eed5f4dd44478b6bdb307b629535b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1786https://doaj.org/toc/2076-3921<i>Opuntia stricta</i> var. <i>Dillenii</i>’s prickly pears are an underutilized fruit with a high content of betalains and phenolic compounds that could bring potential health benefits for humans. The aim of this study is the optimization of the “green” extraction of betalains and phenolic compounds from <i>Opuntia stricta</i> var. <i>Dillenii</i>’s whole fruits by ultrasound-assisted extraction (UAE), using a response surface methodology (RSM) by a central composite design (CCD) in order to obtain extracts rich in betalains and phenolic compounds with proven biological activities. For UAE optimization, the extraction temperature (20–50 °C), the amplitude (20–50%) and the ethanol volume in extraction solvent (15–80%, <i>v</i>/<i>v</i>) were selected as independent variables. All combinations were conducted at 2, 5, 10, 20 and 30 min to determinate the time effect. The betalain and phenolic compound content in <i>Opuntia stricta</i> var. <i>Dillenii</i>’s whole fruits and UAE extracts were identified by HPLC-DAD-ESI/MS and HPLC-DAD-MS/QTOF and the antioxidant (ORAC method) and the anti-inflammatory (hyaluronidase inhibition method) in vitro biological activities also were determined. The most efficient extraction time was 5 min and the best UAE parameter combination was 50% amplitude, 15% ethanol in solvent (ethanol/water, 15/85, <i>v</i>/<i>v</i>) and 20 °C temperature, obtaining 10.06 ± 0.10 mg of total major betalains/g dry weight, 2.32 ± 0.08 mg of piscidic acid/g dry weight and 0.38 ± 0.00 mg of total major flavonoids/g dry weight. All applied UAE combinations significantly improved the in vitro bioactive activities (antioxidant and anti-inflammatory) of the <i>Opuntia stricta</i> var. <i>Dillenii</i>’s extracts compared to the bioactivities of the extracts obtained by standard homogenization processes.Iván Gómez-LópezGloria Lobo-RodrigoMaría P. PortilloM. Pilar CanoMDPI AGarticle<i>Opuntia stricta</i> var. <i>Dillenii</i> whole fruitsultrasound-assisted extractiongreen solventsbetalainsphenolic compoundsantioxidant activityTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1786, p 1786 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Opuntia stricta</i> var. <i>Dillenii</i> whole fruits ultrasound-assisted extraction green solvents betalains phenolic compounds antioxidant activity Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Opuntia stricta</i> var. <i>Dillenii</i> whole fruits ultrasound-assisted extraction green solvents betalains phenolic compounds antioxidant activity Therapeutics. Pharmacology RM1-950 Iván Gómez-López Gloria Lobo-Rodrigo María P. Portillo M. Pilar Cano Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities
description	<i>Opuntia stricta</i> var. <i>Dillenii</i>’s prickly pears are an underutilized fruit with a high content of betalains and phenolic compounds that could bring potential health benefits for humans. The aim of this study is the optimization of the “green” extraction of betalains and phenolic compounds from <i>Opuntia stricta</i> var. <i>Dillenii</i>’s whole fruits by ultrasound-assisted extraction (UAE), using a response surface methodology (RSM) by a central composite design (CCD) in order to obtain extracts rich in betalains and phenolic compounds with proven biological activities. For UAE optimization, the extraction temperature (20–50 °C), the amplitude (20–50%) and the ethanol volume in extraction solvent (15–80%, <i>v</i>/<i>v</i>) were selected as independent variables. All combinations were conducted at 2, 5, 10, 20 and 30 min to determinate the time effect. The betalain and phenolic compound content in <i>Opuntia stricta</i> var. <i>Dillenii</i>’s whole fruits and UAE extracts were identified by HPLC-DAD-ESI/MS and HPLC-DAD-MS/QTOF and the antioxidant (ORAC method) and the anti-inflammatory (hyaluronidase inhibition method) in vitro biological activities also were determined. The most efficient extraction time was 5 min and the best UAE parameter combination was 50% amplitude, 15% ethanol in solvent (ethanol/water, 15/85, <i>v</i>/<i>v</i>) and 20 °C temperature, obtaining 10.06 ± 0.10 mg of total major betalains/g dry weight, 2.32 ± 0.08 mg of piscidic acid/g dry weight and 0.38 ± 0.00 mg of total major flavonoids/g dry weight. All applied UAE combinations significantly improved the in vitro bioactive activities (antioxidant and anti-inflammatory) of the <i>Opuntia stricta</i> var. <i>Dillenii</i>’s extracts compared to the bioactivities of the extracts obtained by standard homogenization processes.
format	article
author	Iván Gómez-López Gloria Lobo-Rodrigo María P. Portillo M. Pilar Cano
author_facet	Iván Gómez-López Gloria Lobo-Rodrigo María P. Portillo M. Pilar Cano
author_sort	Iván Gómez-López
title	Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities
title_short	Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities
title_full	Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities
title_fullStr	Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities
title_full_unstemmed	Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities
title_sort	ultrasound-assisted “green” extraction (uae) of antioxidant compounds (betalains and phenolics) from <i>opuntia stricta</i> var. <i>dilenii</i>’s fruits: optimization and biological activities
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/256eed5f4dd44478b6bdb307b629535b
work_keys_str_mv	AT ivangomezlopez ultrasoundassistedgreenextractionuaeofantioxidantcompoundsbetalainsandphenolicsfromiopuntiastrictaivaridileniiisfruitsoptimizationandbiologicalactivities AT glorialoborodrigo ultrasoundassistedgreenextractionuaeofantioxidantcompoundsbetalainsandphenolicsfromiopuntiastrictaivaridileniiisfruitsoptimizationandbiologicalactivities AT mariapportillo ultrasoundassistedgreenextractionuaeofantioxidantcompoundsbetalainsandphenolicsfromiopuntiastrictaivaridileniiisfruitsoptimizationandbiologicalactivities AT mpilarcano ultrasoundassistedgreenextractionuaeofantioxidantcompoundsbetalainsandphenolicsfromiopuntiastrictaivaridileniiisfruitsoptimizationandbiologicalactivities
_version_	1718413124546068480

Ultrasound-Assisted “Green” Extraction (UAE) of Antioxidant Compounds (Betalains and Phenolics) from <i>Opuntia stricta</i> var. <i>Dilenii</i>’s Fruits: Optimization and Biological Activities

Ejemplares similares