Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats

Chenmeng Li,1–3,* Xiaoyan Xu,2–4,* Xiong Zhang,2–4,* Ke Cheng,2,3,* Yujie Guo,1–3 Jie Jie,1–3 Hua Guo,1–3 Yong He,1–3 Chanjuan Zhou,2,3 Siwen Gui,2,3 Xiaogang Zhong,2,3 Haiyang Wang,2–4 Peng Xie1–4 1Department...

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Autores principales:	Li C, Xu X, Zhang X, Cheng K, Guo Y, Jie J, Guo H, He Y, Zhou C, Gui S, Zhong X, Wang H, Xie P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Borna disease virus ERK/CREB/BDNF signaling neonatal Borna disease Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/257636abcc9841388e5625197db98a21
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spelling	oai:doaj.org-article:257636abcc9841388e5625197db98a212021-12-02T04:18:20ZActivation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats1178-2021https://doaj.org/article/257636abcc9841388e5625197db98a212018-11-01T00:00:00Zhttps://www.dovepress.com/activation-of-erkcrebbdnf-pathway-involved-in-abnormal-behavior-of-neo-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Chenmeng Li,1–3,* Xiaoyan Xu,2–4,* Xiong Zhang,2–4,* Ke Cheng,2,3,* Yujie Guo,1–3 Jie Jie,1–3 Hua Guo,1–3 Yong He,1–3 Chanjuan Zhou,2,3 Siwen Gui,2,3 Xiaogang Zhong,2,3 Haiyang Wang,2–4 Peng Xie1–4 1Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; 2Department of Neurology Institute of Neuroscience and Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; 3Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; 4Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China *These authors contributed equally to this work Background: Neuropsychiatric disorders are devastating illnesses worldwide; however, the potential involvement of viruses in the pathophysiological mechanisms of psychiatric diseases have not been clearly elucidated. Borna disease virus (BDV) is a neurotropic, noncytopathic RNA virus. Materials and methods: In this study, we infected neonatal rats intracranially with BDV Hu-H1 and Strain V within 24 hours of birth. Psychological phenotypes were assessed using sucrose preference test, open field test, elevated plus maze test, and forced swim test. The protein expression of ERK/CREB/BDNF pathway was assessed by Western blotting of in vitro and in vivo samples. Results: Hu-H1-infected rats showed anxiety-like behavior 8 weeks postinfection while Strain V-infected rats demonstrated a certain abnormal behavior. Phosphorylated ERK1/2 was significantly upregulated in the hippocampi of Strain V- and Hu-H1-infected rats compared with control rats, indicating that Raf/MEK/ERK signaling was activated. Conclusion: The data suggested that infection of neonatal rats with BDV Hu-H1 and Strain V caused behavioral abnormalities that shared common molecular pathways, providing preliminary evidences to investigate the underlying mechanisms of psychiatric disorders caused by BDV. Keywords: Borna disease virus, ERK/CREB/BDNF signaling pathway, neonatal Borna diseaseLi CXu XZhang XCheng KGuo YJie JGuo HHe YZhou CGui SZhong XWang HXie PDove Medical PressarticleBorna disease virusERK/CREB/BDNF signalingneonatal Borna diseaseNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 14, Pp 3121-3132 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Borna disease virus ERK/CREB/BDNF signaling neonatal Borna disease Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Borna disease virus ERK/CREB/BDNF signaling neonatal Borna disease Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Li C Xu X Zhang X Cheng K Guo Y Jie J Guo H He Y Zhou C Gui S Zhong X Wang H Xie P Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats
description	Chenmeng Li,1–3,* Xiaoyan Xu,2–4,* Xiong Zhang,2–4,* Ke Cheng,2,3,* Yujie Guo,1–3 Jie Jie,1–3 Hua Guo,1–3 Yong He,1–3 Chanjuan Zhou,2,3 Siwen Gui,2,3 Xiaogang Zhong,2,3 Haiyang Wang,2–4 Peng Xie1–4 1Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; 2Department of Neurology Institute of Neuroscience and Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; 3Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; 4Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China *These authors contributed equally to this work Background: Neuropsychiatric disorders are devastating illnesses worldwide; however, the potential involvement of viruses in the pathophysiological mechanisms of psychiatric diseases have not been clearly elucidated. Borna disease virus (BDV) is a neurotropic, noncytopathic RNA virus. Materials and methods: In this study, we infected neonatal rats intracranially with BDV Hu-H1 and Strain V within 24 hours of birth. Psychological phenotypes were assessed using sucrose preference test, open field test, elevated plus maze test, and forced swim test. The protein expression of ERK/CREB/BDNF pathway was assessed by Western blotting of in vitro and in vivo samples. Results: Hu-H1-infected rats showed anxiety-like behavior 8 weeks postinfection while Strain V-infected rats demonstrated a certain abnormal behavior. Phosphorylated ERK1/2 was significantly upregulated in the hippocampi of Strain V- and Hu-H1-infected rats compared with control rats, indicating that Raf/MEK/ERK signaling was activated. Conclusion: The data suggested that infection of neonatal rats with BDV Hu-H1 and Strain V caused behavioral abnormalities that shared common molecular pathways, providing preliminary evidences to investigate the underlying mechanisms of psychiatric disorders caused by BDV. Keywords: Borna disease virus, ERK/CREB/BDNF signaling pathway, neonatal Borna disease
format	article
author	Li C Xu X Zhang X Cheng K Guo Y Jie J Guo H He Y Zhou C Gui S Zhong X Wang H Xie P
author_facet	Li C Xu X Zhang X Cheng K Guo Y Jie J Guo H He Y Zhou C Gui S Zhong X Wang H Xie P
author_sort	Li C
title	Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats
title_short	Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats
title_full	Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats
title_fullStr	Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats
title_full_unstemmed	Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats
title_sort	activation of erk/creb/bdnf pathway involved in abnormal behavior of neonatally borna virus-infected rats
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/257636abcc9841388e5625197db98a21
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Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats

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