Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to elect...

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Autores principales:	Mi Hee Lee, Ye Jin Park, Seung Hee Hong, Min-Ah Koo, Minyoung Cho, Jong-Chul Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrotaxis directional migration adipose-derived stem cells Golgi polarization Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2579542c3778459c83afbc7fb6995dc6
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spelling	oai:doaj.org-article:2579542c3778459c83afbc7fb6995dc62021-11-25T17:07:53ZPulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells10.3390/cells101128462073-4409https://doaj.org/article/2579542c3778459c83afbc7fb6995dc62021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2846https://doaj.org/toc/2073-4409Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.Mi Hee LeeYe Jin ParkSeung Hee HongMin-Ah KooMinyoung ChoJong-Chul ParkMDPI AGarticleelectrotaxisdirectional migrationadipose-derived stem cellsGolgi polarizationBiology (General)QH301-705.5ENCells, Vol 10, Iss 2846, p 2846 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrotaxis directional migration adipose-derived stem cells Golgi polarization Biology (General) QH301-705.5
spellingShingle	electrotaxis directional migration adipose-derived stem cells Golgi polarization Biology (General) QH301-705.5 Mi Hee Lee Ye Jin Park Seung Hee Hong Min-Ah Koo Minyoung Cho Jong-Chul Park Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
description	Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.
format	article
author	Mi Hee Lee Ye Jin Park Seung Hee Hong Min-Ah Koo Minyoung Cho Jong-Chul Park
author_facet	Mi Hee Lee Ye Jin Park Seung Hee Hong Min-Ah Koo Minyoung Cho Jong-Chul Park
author_sort	Mi Hee Lee
title	Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
title_short	Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
title_full	Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
title_fullStr	Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
title_full_unstemmed	Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
title_sort	pulsed electrical stimulation enhances consistency of directional migration of adipose-derived stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2579542c3778459c83afbc7fb6995dc6
work_keys_str_mv	AT miheelee pulsedelectricalstimulationenhancesconsistencyofdirectionalmigrationofadiposederivedstemcells AT yejinpark pulsedelectricalstimulationenhancesconsistencyofdirectionalmigrationofadiposederivedstemcells AT seungheehong pulsedelectricalstimulationenhancesconsistencyofdirectionalmigrationofadiposederivedstemcells AT minahkoo pulsedelectricalstimulationenhancesconsistencyofdirectionalmigrationofadiposederivedstemcells AT minyoungcho pulsedelectricalstimulationenhancesconsistencyofdirectionalmigrationofadiposederivedstemcells AT jongchulpark pulsedelectricalstimulationenhancesconsistencyofdirectionalmigrationofadiposederivedstemcells
_version_	1718412701108011008

Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

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