Cytotoxicity of gold nanoclusters in human liver cancer cells

Cytotoxicity of gold nanoclusters in human liver cancer cells

Yanjie Yang,1,2 Jing Nan,3 Jianwen Hou,3 Bianfei Yu,1 Tong Zhao,1 Shuang Xu,1 Shuangyu Lv,2 Haixia Zhang1 1Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou, 2School of Medicine, Henan University, Kaifeng, 3School of Life Sciences...

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Autores principales: Yang Y, Nan J, Hou J, Yu B, Zhao T, Xu S, Lv S, Zhang H
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/258bd8dfe70740bdba52fd5d9ce9b9de
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spelling oai:doaj.org-article:258bd8dfe70740bdba52fd5d9ce9b9de2021-12-02T00:13:00ZCytotoxicity of gold nanoclusters in human liver cancer cells1178-2013https://doaj.org/article/258bd8dfe70740bdba52fd5d9ce9b9de2014-11-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-of-gold-nanoclusters-in-human-livernbspcancer-cells-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Yanjie Yang,1,2 Jing Nan,3 Jianwen Hou,3 Bianfei Yu,1 Tong Zhao,1 Shuang Xu,1 Shuangyu Lv,2 Haixia Zhang1 1Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou, 2School of Medicine, Henan University, Kaifeng, 3School of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of China Abstract: In this study, we synthesized water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with dihydrolipoic acid (DHLA). The cytotoxicity of these Au NCs was then assessed in the normal human hepatic cell line (L02) and the human hepatoma cell line (HepG2) at different exposure times. Cell viability was normal in both cell lines at 24 hours and 48 hours; however, the growth of HepG2 cells was significantly inhibited at 72 hours. The change in lactate dehydrogenase level was strongly correlated with cell viability after 72 hours incubation with DHLA–capped Au NCs, and the increase in cellular reactive oxygen species may be related to the decrease in cell viability. Growth inhibition of HepG2 cells was possibly due to difficultly passing the checkpoint between G1 phase and S phase. The anticancer activity of DHLA–capped Au NCs should be considered when used in biomedical imaging and drug delivery. Keywords: gold nanocluster, cytotoxicity, cell viability, reactive oxygen species, cell cycleYang YNan JHou JYu BZhao TXu SLv SZhang HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5441-5448 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Yang Y
Nan J
Hou J
Yu B
Zhao T
Xu S
Lv S
Zhang H
Cytotoxicity of gold nanoclusters in human liver cancer cells
description Yanjie Yang,1,2 Jing Nan,3 Jianwen Hou,3 Bianfei Yu,1 Tong Zhao,1 Shuang Xu,1 Shuangyu Lv,2 Haixia Zhang1 1Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou, 2School of Medicine, Henan University, Kaifeng, 3School of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of China Abstract: In this study, we synthesized water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with dihydrolipoic acid (DHLA). The cytotoxicity of these Au NCs was then assessed in the normal human hepatic cell line (L02) and the human hepatoma cell line (HepG2) at different exposure times. Cell viability was normal in both cell lines at 24 hours and 48 hours; however, the growth of HepG2 cells was significantly inhibited at 72 hours. The change in lactate dehydrogenase level was strongly correlated with cell viability after 72 hours incubation with DHLA–capped Au NCs, and the increase in cellular reactive oxygen species may be related to the decrease in cell viability. Growth inhibition of HepG2 cells was possibly due to difficultly passing the checkpoint between G1 phase and S phase. The anticancer activity of DHLA–capped Au NCs should be considered when used in biomedical imaging and drug delivery. Keywords: gold nanocluster, cytotoxicity, cell viability, reactive oxygen species, cell cycle
format article
author Yang Y
Nan J
Hou J
Yu B
Zhao T
Xu S
Lv S
Zhang H
author_facet Yang Y
Nan J
Hou J
Yu B
Zhao T
Xu S
Lv S
Zhang H
author_sort Yang Y
title Cytotoxicity of gold nanoclusters in human liver cancer cells
title_short Cytotoxicity of gold nanoclusters in human liver cancer cells
title_full Cytotoxicity of gold nanoclusters in human liver cancer cells
title_fullStr Cytotoxicity of gold nanoclusters in human liver cancer cells
title_full_unstemmed Cytotoxicity of gold nanoclusters in human liver cancer cells
title_sort cytotoxicity of gold nanoclusters in human liver cancer cells
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/258bd8dfe70740bdba52fd5d9ce9b9de
work_keys_str_mv AT yangy cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT nanj cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT houj cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT yub cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT zhaot cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT xus cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT lvs cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
AT zhangh cytotoxicityofgoldnanoclustersinhumanlivernbspcancercells
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