Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro

Fei Gao,1,2 Zhiqiang Liu,3 Wei Ren,3 Wen Jiang1 1Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, People’s Republic of China; 2Department of Neurology, First Affiliated Hospital of Xi’an Medical University, Xi’an 71...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gao F, Liu Z, Ren W, Jiang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/258efe9f3b8a492b804c65ffd72502c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:258efe9f3b8a492b804c65ffd72502c7
record_format	dspace
spelling	oai:doaj.org-article:258efe9f3b8a492b804c65ffd72502c72021-12-02T01:29:00ZAcute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro1178-2021https://doaj.org/article/258efe9f3b8a492b804c65ffd72502c72014-08-01T00:00:00Zhttp://www.dovepress.com/acute-lipopolysaccharide-exposure-facilitates-epileptiform-activity-vi-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021 Fei Gao,1,2 Zhiqiang Liu,3 Wei Ren,3 Wen Jiang1 1Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, People’s Republic of China; 2Department of Neurology, First Affiliated Hospital of Xi’an Medical University, Xi’an 710077, People’s Republic of China; 3College of Life Sciences, Shaanxi Normal University, Xi’an 710062, People’s Republic of China Abstract: Growing evidence indicates brain inflammation has been involved in the genesis of seizures. However, the direct effect of acute inflammation on neuronal circuits is not well known. Lipopolysaccharide (LPS) has been used extensively to stimulate brain inflammatory responses both in vivo and in vitro. Here, we observed the contribution of inflammation induced by 10 μg/mL LPS to the excitability of neuronal circuits in acute hippocampal slices. When slices were incubated with LPS for 30 minutes, significant increased concentration of tumor necrosis factor α and interleukin 1β were detected by enzyme-linked immunosorbent assay. In electrophysiological recordings, we found that frequency of epileptiform discharges and spikes per burst increased 30 minutes after LPS application. LPS enhanced evoked excitatory postsynaptic currents but did not modify evoked inhibitory postsynaptic currents. In addition, exposure to LPS enhanced the excitability of CA1 pyramidal neurons, as demonstrated by a decrease in rheobase and an increase in action potential frequency elicited by depolarizing current injection. Our observations suggest that acute inflammation induced by LPS facilitates epileptiform activity in vitro and that enhancement of excitatory synaptic transmission and neuronal excitability may contribute to this facilitation. These results may provide new clues for treating seizures associated with brain inflammatory disease. Keywords: lipopolysaccharide, hippocampus, inflammation, epileptiform activity, synaptic transmission, neuronal excitabilityGao FLiu ZRen WJiang WDove Medical PressarticleNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol 2014, Iss default, Pp 1489-1495 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Gao F Liu Z Ren W Jiang W Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
description	Fei Gao,1,2 Zhiqiang Liu,3 Wei Ren,3 Wen Jiang1 1Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, People’s Republic of China; 2Department of Neurology, First Affiliated Hospital of Xi’an Medical University, Xi’an 710077, People’s Republic of China; 3College of Life Sciences, Shaanxi Normal University, Xi’an 710062, People’s Republic of China Abstract: Growing evidence indicates brain inflammation has been involved in the genesis of seizures. However, the direct effect of acute inflammation on neuronal circuits is not well known. Lipopolysaccharide (LPS) has been used extensively to stimulate brain inflammatory responses both in vivo and in vitro. Here, we observed the contribution of inflammation induced by 10 μg/mL LPS to the excitability of neuronal circuits in acute hippocampal slices. When slices were incubated with LPS for 30 minutes, significant increased concentration of tumor necrosis factor α and interleukin 1β were detected by enzyme-linked immunosorbent assay. In electrophysiological recordings, we found that frequency of epileptiform discharges and spikes per burst increased 30 minutes after LPS application. LPS enhanced evoked excitatory postsynaptic currents but did not modify evoked inhibitory postsynaptic currents. In addition, exposure to LPS enhanced the excitability of CA1 pyramidal neurons, as demonstrated by a decrease in rheobase and an increase in action potential frequency elicited by depolarizing current injection. Our observations suggest that acute inflammation induced by LPS facilitates epileptiform activity in vitro and that enhancement of excitatory synaptic transmission and neuronal excitability may contribute to this facilitation. These results may provide new clues for treating seizures associated with brain inflammatory disease. Keywords: lipopolysaccharide, hippocampus, inflammation, epileptiform activity, synaptic transmission, neuronal excitability
format	article
author	Gao F Liu Z Ren W Jiang W
author_facet	Gao F Liu Z Ren W Jiang W
author_sort	Gao F
title	Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
title_short	Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
title_full	Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
title_fullStr	Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
title_full_unstemmed	Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
title_sort	acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/258efe9f3b8a492b804c65ffd72502c7
work_keys_str_mv	AT gaof acutelipopolysaccharideexposurefacilitatesepileptiformactivityviaenhancedexcitatorysynaptictransmissionandneuronalexcitabilityinvitro AT liuz acutelipopolysaccharideexposurefacilitatesepileptiformactivityviaenhancedexcitatorysynaptictransmissionandneuronalexcitabilityinvitro AT renw acutelipopolysaccharideexposurefacilitatesepileptiformactivityviaenhancedexcitatorysynaptictransmissionandneuronalexcitabilityinvitro AT jiangw acutelipopolysaccharideexposurefacilitatesepileptiformactivityviaenhancedexcitatorysynaptictransmissionandneuronalexcitabilityinvitro
_version_	1718403061837201408

Acute lipopolysaccharide exposure facilitates epileptiform activity via enhanced excitatory synaptic transmission and neuronal excitability in vitro

Ejemplares similares