Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models

Abstract Organotypic skin tissue models have decades of use for basic research applications, the treatment of burns, and for efficacy/safety evaluation studies. The complex and heterogeneous nature of native human skin however creates difficulties for the construction of physiologically comparable o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sabrina Madiedo-Podvrsan, Jean-Philippe Belaïdi, Stephanie Desbouis, Lucie Simonetti, Youcef Ben-Khalifa, Jérémie Soeur, Maïté Rielland
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/25902d4945754438aa8651e5d28d1799
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:25902d4945754438aa8651e5d28d1799
record_format	dspace
spelling	oai:doaj.org-article:25902d4945754438aa8651e5d28d17992021-12-02T17:05:49ZUtilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models10.1038/s41598-021-85553-32045-2322https://doaj.org/article/25902d4945754438aa8651e5d28d17992021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85553-3https://doaj.org/toc/2045-2322Abstract Organotypic skin tissue models have decades of use for basic research applications, the treatment of burns, and for efficacy/safety evaluation studies. The complex and heterogeneous nature of native human skin however creates difficulties for the construction of physiologically comparable organotypic models. Within the present study, we utilized bioprinting technology for the controlled deposition of separate keratinocyte subpopulations to create a reconstructed epidermis with two distinct halves in a single insert, each comprised of a different keratinocyte sub-population, in order to better model heterogonous skin and reduce inter-sample variability. As an initial proof-of-concept, we created a patterned epidermal skin model using GPF positive and negative keratinocyte subpopulations, both printed into 2 halves of a reconstructed skin insert, demonstrating the feasibility of this approach. We then demonstrated the physiological relevance of this bioprinting technique by generating a heterogeneous model comprised of dual keratinocyte population with either normal or low filaggrin expression. The resultant model exhibited a well-organized epidermal structure with each half possessing the phenotypic characteristics of its constituent cells, indicative of a successful and stable tissue reconstruction. This patterned skin model aims to mimic the edge of lesions as seen in atopic dermatitis or ichthyosis vulgaris, while the use of two populations within a single insert allows for paired statistics in evaluation studies, likely increasing study statistical power and reducing the number of models required per study. This is the first report of human patterned epidermal model using a predefined bioprinted designs, and demonstrates the relevance of bioprinting to faithfully reproduce human skin microanatomy.Sabrina Madiedo-PodvrsanJean-Philippe BelaïdiStephanie DesbouisLucie SimonettiYoucef Ben-KhalifaJérémie SoeurMaïté RiellandNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabrina Madiedo-Podvrsan Jean-Philippe Belaïdi Stephanie Desbouis Lucie Simonetti Youcef Ben-Khalifa Jérémie Soeur Maïté Rielland Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
description	Abstract Organotypic skin tissue models have decades of use for basic research applications, the treatment of burns, and for efficacy/safety evaluation studies. The complex and heterogeneous nature of native human skin however creates difficulties for the construction of physiologically comparable organotypic models. Within the present study, we utilized bioprinting technology for the controlled deposition of separate keratinocyte subpopulations to create a reconstructed epidermis with two distinct halves in a single insert, each comprised of a different keratinocyte sub-population, in order to better model heterogonous skin and reduce inter-sample variability. As an initial proof-of-concept, we created a patterned epidermal skin model using GPF positive and negative keratinocyte subpopulations, both printed into 2 halves of a reconstructed skin insert, demonstrating the feasibility of this approach. We then demonstrated the physiological relevance of this bioprinting technique by generating a heterogeneous model comprised of dual keratinocyte population with either normal or low filaggrin expression. The resultant model exhibited a well-organized epidermal structure with each half possessing the phenotypic characteristics of its constituent cells, indicative of a successful and stable tissue reconstruction. This patterned skin model aims to mimic the edge of lesions as seen in atopic dermatitis or ichthyosis vulgaris, while the use of two populations within a single insert allows for paired statistics in evaluation studies, likely increasing study statistical power and reducing the number of models required per study. This is the first report of human patterned epidermal model using a predefined bioprinted designs, and demonstrates the relevance of bioprinting to faithfully reproduce human skin microanatomy.
format	article
author	Sabrina Madiedo-Podvrsan Jean-Philippe Belaïdi Stephanie Desbouis Lucie Simonetti Youcef Ben-Khalifa Jérémie Soeur Maïté Rielland
author_facet	Sabrina Madiedo-Podvrsan Jean-Philippe Belaïdi Stephanie Desbouis Lucie Simonetti Youcef Ben-Khalifa Jérémie Soeur Maïté Rielland
author_sort	Sabrina Madiedo-Podvrsan
title	Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
title_short	Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
title_full	Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
title_fullStr	Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
title_full_unstemmed	Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
title_sort	utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/25902d4945754438aa8651e5d28d1799
work_keys_str_mv	AT sabrinamadiedopodvrsan utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels AT jeanphilippebelaidi utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels AT stephaniedesbouis utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels AT luciesimonetti utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels AT youcefbenkhalifa utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels AT jeremiesoeur utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels AT maiterielland utilizationofpatternedbioprintingforheterogeneousandphysiologicallyrepresentativereconstructedepidermalskinmodels
_version_	1718381779449020416

Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models

Ejemplares similares