Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong

Pneumococcal conjugate vaccines (PCVs) successfully decreased the incidence of invasive pneumococcal disease in children. However, many countries have reported serotype replacement and a rebound in diseases from non-vaccine serotypes. Here, we report the genomic investigation of a Streptococcus pneu...

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Autores principales:	Huiluo Cao, Kelvin Hei-Yeung Chiu, Susan S. Chiu, Shuo Jiang, Kin-Hung Chow, Pak-Leung Ho
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Pneumococcal conjugate vaccine Epidemiology Invasive pneumococcal disease Cotrimoxazole resistance Microbiology QR1-502 Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/25949474bad64117946faf67699c02bc
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spelling	oai:doaj.org-article:25949474bad64117946faf67699c02bc2021-12-04T04:33:24ZGenomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong1438-422110.1016/j.ijmm.2021.151543https://doaj.org/article/25949474bad64117946faf67699c02bc2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1438422121000722https://doaj.org/toc/1438-4221Pneumococcal conjugate vaccines (PCVs) successfully decreased the incidence of invasive pneumococcal disease in children. However, many countries have reported serotype replacement and a rebound in diseases from non-vaccine serotypes. Here, we report the genomic investigation of a Streptococcus pneumoniae strain M215 that caused severe meningoencephalitis in an infant in 2019. The strain was assigned to serotype 24F using the bioinformatic pipeline SeroBA and pneumococcal type specific anti-sera. The strain was resistant to cotrimoxazole from mutations in both folA and folP genes. It was susceptible to penicillin and other non-β-lactam antibiotics. Phylogenetically, it belongs to Global Pneumococcal Sequence Cluster (GPSC) 6 and multi-locus sequence type 162. A total of 38 virulence genes were detected in the genome of M215. Upon comparison of the profile of virulence genes, GPSC6 but not non-GPSC6 strains of serotype 24F and related serotypes were found to possess the major virulence determinant, pilus islet-1, comprising genes encoding sortases (srtB, srtC, srtD), pilus proteins (rrgA, rrgB and rrgC) and one transcriptional regulator (rlrA), which was previously described to be characteristic feature of international clones in the pre-PCV era. In our locality, this represented the first detection of serotype 24F and GPSC6/ST162 causing serious pneumococcal disease. The emergence of the non-vaccine serotype 24F GPSC6/ST162 lineage with molecular feature of high virulence is concerning and emphasizes the need for full characterization of strains causing severe disease.Huiluo CaoKelvin Hei-Yeung ChiuSusan S. ChiuShuo JiangKin-Hung ChowPak-Leung HoElsevierarticlePneumococcal conjugate vaccineEpidemiologyInvasive pneumococcal diseaseCotrimoxazole resistanceMicrobiologyQR1-502Other systems of medicineRZ201-999ENInternational Journal of Medical Microbiology, Vol 311, Iss 8, Pp 151543- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pneumococcal conjugate vaccine Epidemiology Invasive pneumococcal disease Cotrimoxazole resistance Microbiology QR1-502 Other systems of medicine RZ201-999
spellingShingle	Pneumococcal conjugate vaccine Epidemiology Invasive pneumococcal disease Cotrimoxazole resistance Microbiology QR1-502 Other systems of medicine RZ201-999 Huiluo Cao Kelvin Hei-Yeung Chiu Susan S. Chiu Shuo Jiang Kin-Hung Chow Pak-Leung Ho Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
description	Pneumococcal conjugate vaccines (PCVs) successfully decreased the incidence of invasive pneumococcal disease in children. However, many countries have reported serotype replacement and a rebound in diseases from non-vaccine serotypes. Here, we report the genomic investigation of a Streptococcus pneumoniae strain M215 that caused severe meningoencephalitis in an infant in 2019. The strain was assigned to serotype 24F using the bioinformatic pipeline SeroBA and pneumococcal type specific anti-sera. The strain was resistant to cotrimoxazole from mutations in both folA and folP genes. It was susceptible to penicillin and other non-β-lactam antibiotics. Phylogenetically, it belongs to Global Pneumococcal Sequence Cluster (GPSC) 6 and multi-locus sequence type 162. A total of 38 virulence genes were detected in the genome of M215. Upon comparison of the profile of virulence genes, GPSC6 but not non-GPSC6 strains of serotype 24F and related serotypes were found to possess the major virulence determinant, pilus islet-1, comprising genes encoding sortases (srtB, srtC, srtD), pilus proteins (rrgA, rrgB and rrgC) and one transcriptional regulator (rlrA), which was previously described to be characteristic feature of international clones in the pre-PCV era. In our locality, this represented the first detection of serotype 24F and GPSC6/ST162 causing serious pneumococcal disease. The emergence of the non-vaccine serotype 24F GPSC6/ST162 lineage with molecular feature of high virulence is concerning and emphasizes the need for full characterization of strains causing severe disease.
format	article
author	Huiluo Cao Kelvin Hei-Yeung Chiu Susan S. Chiu Shuo Jiang Kin-Hung Chow Pak-Leung Ho
author_facet	Huiluo Cao Kelvin Hei-Yeung Chiu Susan S. Chiu Shuo Jiang Kin-Hung Chow Pak-Leung Ho
author_sort	Huiluo Cao
title	Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
title_short	Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
title_full	Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
title_fullStr	Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
title_full_unstemmed	Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong
title_sort	genomic investigation of a streptococcus pneumoniae serotype 24f strain causing meningoencephalitis in hong kong
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/25949474bad64117946faf67699c02bc
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Genomic investigation of a Streptococcus pneumoniae serotype 24F strain causing meningoencephalitis in Hong Kong

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