A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.

<h4>Background</h4>DNA sequences from ancient specimens may in fact result from undetected contamination of the ancient specimens by modern DNA, and the problem is particularly challenging in studies of human fossils. Doubts on the authenticity of the available sequences have so far hamp...

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Autores principales:	David Caramelli, Lucio Milani, Stefania Vai, Alessandra Modi, Elena Pecchioli, Matteo Girardi, Elena Pilli, Martina Lari, Barbara Lippi, Annamaria Ronchitelli, Francesco Mallegni, Antonella Casoli, Giorgio Bertorelle, Guido Barbujani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/25954a904b49410099d7fb470d834f79
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spelling	oai:doaj.org-article:25954a904b49410099d7fb470d834f792021-11-25T06:11:37ZA 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.1932-620310.1371/journal.pone.0002700https://doaj.org/article/25954a904b49410099d7fb470d834f792008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18628960/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>DNA sequences from ancient specimens may in fact result from undetected contamination of the ancient specimens by modern DNA, and the problem is particularly challenging in studies of human fossils. Doubts on the authenticity of the available sequences have so far hampered genetic comparisons between anatomically archaic (Neandertal) and early modern (Cro-Magnoid) Europeans.<h4>Methodology/principal findings</h4>We typed the mitochondrial DNA (mtDNA) hypervariable region I in a 28,000 years old Cro-Magnoid individual from the Paglicci cave, in Italy (Paglicci 23) and in all the people who had contact with the sample since its discovery in 2003. The Paglicci 23 sequence, determined through the analysis of 152 clones, is the Cambridge reference sequence, and cannot possibly reflect contamination because it differs from all potentially contaminating modern sequences.<h4>Conclusions/significance</h4>The Paglicci 23 individual carried a mtDNA sequence that is still common in Europe, and which radically differs from those of the almost contemporary Neandertals, demonstrating a genealogical continuity across 28,000 years, from Cro-Magnoid to modern Europeans. Because all potential sources of modern DNA contamination are known, the Paglicci 23 sample will offer a unique opportunity to get insight for the first time into the nuclear genes of early modern Europeans.David CaramelliLucio MilaniStefania VaiAlessandra ModiElena PecchioliMatteo GirardiElena PilliMartina LariBarbara LippiAnnamaria RonchitelliFrancesco MallegniAntonella CasoliGiorgio BertorelleGuido BarbujaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 7, p e2700 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Caramelli Lucio Milani Stefania Vai Alessandra Modi Elena Pecchioli Matteo Girardi Elena Pilli Martina Lari Barbara Lippi Annamaria Ronchitelli Francesco Mallegni Antonella Casoli Giorgio Bertorelle Guido Barbujani A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.
description	<h4>Background</h4>DNA sequences from ancient specimens may in fact result from undetected contamination of the ancient specimens by modern DNA, and the problem is particularly challenging in studies of human fossils. Doubts on the authenticity of the available sequences have so far hampered genetic comparisons between anatomically archaic (Neandertal) and early modern (Cro-Magnoid) Europeans.<h4>Methodology/principal findings</h4>We typed the mitochondrial DNA (mtDNA) hypervariable region I in a 28,000 years old Cro-Magnoid individual from the Paglicci cave, in Italy (Paglicci 23) and in all the people who had contact with the sample since its discovery in 2003. The Paglicci 23 sequence, determined through the analysis of 152 clones, is the Cambridge reference sequence, and cannot possibly reflect contamination because it differs from all potentially contaminating modern sequences.<h4>Conclusions/significance</h4>The Paglicci 23 individual carried a mtDNA sequence that is still common in Europe, and which radically differs from those of the almost contemporary Neandertals, demonstrating a genealogical continuity across 28,000 years, from Cro-Magnoid to modern Europeans. Because all potential sources of modern DNA contamination are known, the Paglicci 23 sample will offer a unique opportunity to get insight for the first time into the nuclear genes of early modern Europeans.
format	article
author	David Caramelli Lucio Milani Stefania Vai Alessandra Modi Elena Pecchioli Matteo Girardi Elena Pilli Martina Lari Barbara Lippi Annamaria Ronchitelli Francesco Mallegni Antonella Casoli Giorgio Bertorelle Guido Barbujani
author_facet	David Caramelli Lucio Milani Stefania Vai Alessandra Modi Elena Pecchioli Matteo Girardi Elena Pilli Martina Lari Barbara Lippi Annamaria Ronchitelli Francesco Mallegni Antonella Casoli Giorgio Bertorelle Guido Barbujani
author_sort	David Caramelli
title	A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.
title_short	A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.
title_full	A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.
title_fullStr	A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.
title_full_unstemmed	A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.
title_sort	28,000 years old cro-magnon mtdna sequence differs from all potentially contaminating modern sequences.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/25954a904b49410099d7fb470d834f79
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A 28,000 years old Cro-Magnon mtDNA sequence differs from all potentially contaminating modern sequences.

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