Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe

Fumonisins (FB) are mycotoxins known to exert most of their toxicity by blocking ceramide synthase, resulting in disruption of sphingolipid metabolism. Although the effects of FB on sphinganine (Sa) and sphingosine (So) are well documented in poultry, little information is available on their other e...

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Autores principales:	Didier Tardieu, Maria Matard-Mann, Pi Nyvall Collén, Philippe Guerre
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fumonisin sphinganine sphingosine ceramide sphingomyelin sphingolipid Medicine R
Acceso en línea:	https://doaj.org/article/25a175157cf640f4b000a519d7389048
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spelling	oai:doaj.org-article:25a175157cf640f4b000a519d73890482021-11-25T19:08:42ZStrong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe10.3390/toxins131107702072-6651https://doaj.org/article/25a175157cf640f4b000a519d73890482021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/770https://doaj.org/toc/2072-6651Fumonisins (FB) are mycotoxins known to exert most of their toxicity by blocking ceramide synthase, resulting in disruption of sphingolipid metabolism. Although the effects of FB on sphinganine (Sa) and sphingosine (So) are well documented in poultry, little information is available on their other effects on sphingolipids. The objective of this study was to analyze the effects of FB on the hepatic and plasma sphingolipidome in chickens. The first concern of this analysis was to clarify the effects of FB on hepatic sphingolipid levels, whose variations can lead to numerous toxic manifestations. The second was to specify the possible use of an alteration of the sphingolipidome as a biomarker of exposure to FB, in addition to the measurement of the Sa:So ratio already widely used. For this purpose, we developed an UHPLC MS/MS method that enabled the determination of 82 SL, including 10 internal standards, in chicken liver and plasma. The validated method was used to measure the effects of FB administered to chickens at a dose close to 20 mg FB1 + FB2/kg feed for 9 days. Significant alterations of sphingoid bases, ceramides, dihydroceramides, glycosylceramides, sphingomyelins and dihydrosphingomyelins were observed in the liver. In addition, significant increases in plasma sphinganine 1-phosphate, sphingosine 1-phosphate and sphingomyelins were observed in plasma. Interestingly, partial least-squares discriminant analysis of 11 SL in plasma made it possible to discriminate exposed chickens from control chickens, whereas analysis of Sa and So alone revealed no difference. In conclusion, our results show that the effects of FB in chickens are complex, and that SL profiling enables the detection of exposure to FB when Sa and So fail.Didier TardieuMaria Matard-MannPi Nyvall CollénPhilippe GuerreMDPI AGarticlefumonisinsphinganinesphingosineceramidesphingomyelinsphingolipidMedicineRENToxins, Vol 13, Iss 770, p 770 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fumonisin sphinganine sphingosine ceramide sphingomyelin sphingolipid Medicine R
spellingShingle	fumonisin sphinganine sphingosine ceramide sphingomyelin sphingolipid Medicine R Didier Tardieu Maria Matard-Mann Pi Nyvall Collén Philippe Guerre Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe
description	Fumonisins (FB) are mycotoxins known to exert most of their toxicity by blocking ceramide synthase, resulting in disruption of sphingolipid metabolism. Although the effects of FB on sphinganine (Sa) and sphingosine (So) are well documented in poultry, little information is available on their other effects on sphingolipids. The objective of this study was to analyze the effects of FB on the hepatic and plasma sphingolipidome in chickens. The first concern of this analysis was to clarify the effects of FB on hepatic sphingolipid levels, whose variations can lead to numerous toxic manifestations. The second was to specify the possible use of an alteration of the sphingolipidome as a biomarker of exposure to FB, in addition to the measurement of the Sa:So ratio already widely used. For this purpose, we developed an UHPLC MS/MS method that enabled the determination of 82 SL, including 10 internal standards, in chicken liver and plasma. The validated method was used to measure the effects of FB administered to chickens at a dose close to 20 mg FB1 + FB2/kg feed for 9 days. Significant alterations of sphingoid bases, ceramides, dihydroceramides, glycosylceramides, sphingomyelins and dihydrosphingomyelins were observed in the liver. In addition, significant increases in plasma sphinganine 1-phosphate, sphingosine 1-phosphate and sphingomyelins were observed in plasma. Interestingly, partial least-squares discriminant analysis of 11 SL in plasma made it possible to discriminate exposed chickens from control chickens, whereas analysis of Sa and So alone revealed no difference. In conclusion, our results show that the effects of FB in chickens are complex, and that SL profiling enables the detection of exposure to FB when Sa and So fail.
format	article
author	Didier Tardieu Maria Matard-Mann Pi Nyvall Collén Philippe Guerre
author_facet	Didier Tardieu Maria Matard-Mann Pi Nyvall Collén Philippe Guerre
author_sort	Didier Tardieu
title	Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe
title_short	Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe
title_full	Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe
title_fullStr	Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe
title_full_unstemmed	Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe
title_sort	strong alterations in the sphingolipid profile of chickens fed a dose of fumonisins considered safe
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/25a175157cf640f4b000a519d7389048
work_keys_str_mv	AT didiertardieu strongalterationsinthesphingolipidprofileofchickensfedadoseoffumonisinsconsideredsafe AT mariamatardmann strongalterationsinthesphingolipidprofileofchickensfedadoseoffumonisinsconsideredsafe AT pinyvallcollen strongalterationsinthesphingolipidprofileofchickensfedadoseoffumonisinsconsideredsafe AT philippeguerre strongalterationsinthesphingolipidprofileofchickensfedadoseoffumonisinsconsideredsafe
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Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe

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