Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.

Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and...

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Autores principales:	Kateřina Pomajbíková, Miroslav Oborník, Aleš Horák, Klára J Petrželková, J Norman Grim, Bruno Levecke, Angelique Todd, Martin Mulama, John Kiyang, David Modrý
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/25a6fcaff31944438fab70be6dda0781
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spelling	oai:doaj.org-article:25a6fcaff31944438fab70be6dda07812021-11-18T09:15:12ZNovel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.1935-27271935-273510.1371/journal.pntd.0002140https://doaj.org/article/25a6fcaff31944438fab70be6dda07812013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23556024/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes) clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans.Kateřina PomajbíkováMiroslav OborníkAleš HorákKlára J PetrželkováJ Norman GrimBruno LeveckeAngelique ToddMartin MulamaJohn KiyangDavid ModrýPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2140 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
description	Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes) clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans.
format	article
author	Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý
author_facet	Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý
author_sort	Kateřina Pomajbíková
title	Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
title_short	Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
title_full	Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
title_fullStr	Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
title_full_unstemmed	Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
title_sort	novel insights into the genetic diversity of balantidium and balantidium-like cyst-forming ciliates.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/25a6fcaff31944438fab70be6dda0781
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Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.

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