Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

Abstract The purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial prop...

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Autores principales:	Osama A. Madkhali, Sivakumar Sivagurunathan Moni, Muhammad H. Sultan, Haitham A. Bukhary, Mohammed Ghazwani, Nabil A. Alhakamy, Abdulkarim M. Meraya, Saeed Alshahrani, Saad Saeed Alqahtani, Mohammed Ali Bakkari, M. Intakhab Alam, Mohamed Eltaib Elmobark
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/25aa46f901ae4138b7db51e9670d877b
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spelling	oai:doaj.org-article:25aa46f901ae4138b7db51e9670d877b2021-12-02T14:35:39ZFormulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent10.1038/s41598-021-89330-02045-2322https://doaj.org/article/25aa46f901ae4138b7db51e9670d877b2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89330-0https://doaj.org/toc/2045-2322Abstract The purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta potential of − 35.2 mV, particle size of 69.3 z d nm, polydispersity index of 0.6, and percentage polydispersity of 77.8. The DSS nanoparticles were stable up to 102 °C. Differential scanning calorimetry revealed an endothermic peak at 165.77 °C in 12.46 min, while XRD analysis at 2θ depicted various peaks at 21.56°, 33.37°, 38.73°, 47.17°, 52.96°, and 58.42°, indicating discrete nanoparticle formation. Antibacterial studies showed that the DSS nanoparticles were effective against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations of DSS nanoparticles for Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and Proteus vulgaris (P. vulgaris) were 150, 200, 250, 150, 200, 250, 250 µg/mL, respectively. The antibacterial effects of DSS nanoparticles were in the order E. coli (26 ± 1.2 mm) at 150 µg/mL > S. pyogenes (24.6 ± 0.8 mm) at 250 µg/mL > B. subtilis (23.5 ± 2 mm) at 150 µg/mL > K. pneumoniae (22 ± 2 mm) at 250 µg/mL > P. aeruginosa (21.8 ± 1 mm) at 200 µg/mL > S. aureus (20.8 ± 1 mm) at 200 µg/mL > P. vulgaris (20.5 ± 0.9 mm) at 250 µg/mL. These results demonstrate the antibacterial potency of DSS injectable nanoparticles.Osama A. MadkhaliSivakumar Sivagurunathan MoniMuhammad H. SultanHaitham A. BukharyMohammed GhazwaniNabil A. AlhakamyAbdulkarim M. MerayaSaeed AlshahraniSaad Saeed AlqahtaniMohammed Ali BakkariM. Intakhab AlamMohamed Eltaib ElmobarkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Osama A. Madkhali Sivakumar Sivagurunathan Moni Muhammad H. Sultan Haitham A. Bukhary Mohammed Ghazwani Nabil A. Alhakamy Abdulkarim M. Meraya Saeed Alshahrani Saad Saeed Alqahtani Mohammed Ali Bakkari M. Intakhab Alam Mohamed Eltaib Elmobark Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
description	Abstract The purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta potential of − 35.2 mV, particle size of 69.3 z d nm, polydispersity index of 0.6, and percentage polydispersity of 77.8. The DSS nanoparticles were stable up to 102 °C. Differential scanning calorimetry revealed an endothermic peak at 165.77 °C in 12.46 min, while XRD analysis at 2θ depicted various peaks at 21.56°, 33.37°, 38.73°, 47.17°, 52.96°, and 58.42°, indicating discrete nanoparticle formation. Antibacterial studies showed that the DSS nanoparticles were effective against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations of DSS nanoparticles for Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and Proteus vulgaris (P. vulgaris) were 150, 200, 250, 150, 200, 250, 250 µg/mL, respectively. The antibacterial effects of DSS nanoparticles were in the order E. coli (26 ± 1.2 mm) at 150 µg/mL > S. pyogenes (24.6 ± 0.8 mm) at 250 µg/mL > B. subtilis (23.5 ± 2 mm) at 150 µg/mL > K. pneumoniae (22 ± 2 mm) at 250 µg/mL > P. aeruginosa (21.8 ± 1 mm) at 200 µg/mL > S. aureus (20.8 ± 1 mm) at 200 µg/mL > P. vulgaris (20.5 ± 0.9 mm) at 250 µg/mL. These results demonstrate the antibacterial potency of DSS injectable nanoparticles.
format	article
author	Osama A. Madkhali Sivakumar Sivagurunathan Moni Muhammad H. Sultan Haitham A. Bukhary Mohammed Ghazwani Nabil A. Alhakamy Abdulkarim M. Meraya Saeed Alshahrani Saad Saeed Alqahtani Mohammed Ali Bakkari M. Intakhab Alam Mohamed Eltaib Elmobark
author_facet	Osama A. Madkhali Sivakumar Sivagurunathan Moni Muhammad H. Sultan Haitham A. Bukhary Mohammed Ghazwani Nabil A. Alhakamy Abdulkarim M. Meraya Saeed Alshahrani Saad Saeed Alqahtani Mohammed Ali Bakkari M. Intakhab Alam Mohamed Eltaib Elmobark
author_sort	Osama A. Madkhali
title	Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
title_short	Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
title_full	Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
title_fullStr	Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
title_full_unstemmed	Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
title_sort	formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/25aa46f901ae4138b7db51e9670d877b
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Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

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