Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.

The Microviridae comprises icosahedral lytic viruses with circular single-stranded DNA genomes. The family is divided into two distinct groups based on genome characteristics and virion structure. Viruses infecting enterobacteria belong to the genus Microvirus, whereas those infecting obligate paras...

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Autores principales:	Mart Krupovic, Patrick Forterre
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/25ac2cc48d674513990ddebed1644b7f
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spelling	oai:doaj.org-article:25ac2cc48d674513990ddebed1644b7f2021-11-18T06:54:12ZMicroviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.1932-620310.1371/journal.pone.0019893https://doaj.org/article/25ac2cc48d674513990ddebed1644b7f2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21572966/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The Microviridae comprises icosahedral lytic viruses with circular single-stranded DNA genomes. The family is divided into two distinct groups based on genome characteristics and virion structure. Viruses infecting enterobacteria belong to the genus Microvirus, whereas those infecting obligate parasitic bacteria, such as Chlamydia, Spiroplasma and Bdellovibrio, are classified into a subfamily, the Gokushovirinae. Recent metagenomic studies suggest that members of the Microviridae might also play an important role in marine environments. In this study we present the identification and characterization of Microviridae-related prophages integrated in the genomes of species of the Bacteroidetes, a phylum not previously known to be associated with microviruses. Searches against metagenomic databases revealed the presence of highly similar sequences in the human gut. This is the first report indicating that viruses of the Microviridae lysogenize their hosts. Absence of associated integrase-coding genes and apparent recombination with dif-like sequences suggests that Bacteroidetes-associated microviruses are likely to rely on the cellular chromosome dimer resolution machinery. Phylogenetic analysis of the putative major capsid proteins places the identified proviruses into a group separate from the previously characterized microviruses and gokushoviruses, suggesting that the genetic diversity and host range of bacteriophages in the family Microviridae is wider than currently appreciated.Mart KrupovicPatrick ForterrePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19893 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mart Krupovic Patrick Forterre Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.
description	The Microviridae comprises icosahedral lytic viruses with circular single-stranded DNA genomes. The family is divided into two distinct groups based on genome characteristics and virion structure. Viruses infecting enterobacteria belong to the genus Microvirus, whereas those infecting obligate parasitic bacteria, such as Chlamydia, Spiroplasma and Bdellovibrio, are classified into a subfamily, the Gokushovirinae. Recent metagenomic studies suggest that members of the Microviridae might also play an important role in marine environments. In this study we present the identification and characterization of Microviridae-related prophages integrated in the genomes of species of the Bacteroidetes, a phylum not previously known to be associated with microviruses. Searches against metagenomic databases revealed the presence of highly similar sequences in the human gut. This is the first report indicating that viruses of the Microviridae lysogenize their hosts. Absence of associated integrase-coding genes and apparent recombination with dif-like sequences suggests that Bacteroidetes-associated microviruses are likely to rely on the cellular chromosome dimer resolution machinery. Phylogenetic analysis of the putative major capsid proteins places the identified proviruses into a group separate from the previously characterized microviruses and gokushoviruses, suggesting that the genetic diversity and host range of bacteriophages in the family Microviridae is wider than currently appreciated.
format	article
author	Mart Krupovic Patrick Forterre
author_facet	Mart Krupovic Patrick Forterre
author_sort	Mart Krupovic
title	Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.
title_short	Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.
title_full	Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.
title_fullStr	Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.
title_full_unstemmed	Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.
title_sort	microviridae goes temperate: microvirus-related proviruses reside in the genomes of bacteroidetes.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/25ac2cc48d674513990ddebed1644b7f
work_keys_str_mv	AT martkrupovic microviridaegoestemperatemicrovirusrelatedprovirusesresideinthegenomesofbacteroidetes AT patrickforterre microviridaegoestemperatemicrovirusrelatedprovirusesresideinthegenomesofbacteroidetes
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Microviridae goes temperate: microvirus-related proviruses reside in the genomes of Bacteroidetes.

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