Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations

Abstract. Wardatun S, Harahap Y, Mun’im A. 2020. Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations. Biodiversitas 21: 1060-1064. Leucaena leucocephala (Lam.) de Wit (petai Cina), Parkia speciosa Hassk....

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Autores principales:	Sri Wardatun, Yahdiana Harahap, Abdul Mun'im, NOORWATI SUTANDYO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	leguminous seed, solvent, sulfhydryl compound, yield of extract Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/25d02cbddcc849568e661c8bee9cc812
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spelling	oai:doaj.org-article:25d02cbddcc849568e661c8bee9cc8122021-11-22T00:28:56ZShort Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations1412-033X2085-472210.13057/biodiv/d210328https://doaj.org/article/25d02cbddcc849568e661c8bee9cc8122020-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4665https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wardatun S, Harahap Y, Mun’im A. 2020. Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations. Biodiversitas 21: 1060-1064. Leucaena leucocephala (Lam.) de Wit (petai Cina), Parkia speciosa Hassk. (petai), and Archidendron jiringa (Jack) I.C. Nielsen (jengkol) seeds extracts contain sulfhydryl compounds and have various therapeutic properties. The main objective of this research was to compare the effect of various ethanol concentration on the yield of extract and levels of sulfhydryl compounds from leguminous seeds. Dried seeds were macerated at room temperature (25°C) with a ratio of solids and solvents was 1:10. The solvent concentrations used were 30%, 50%, 70% and 96% ethanol. The yield of extract was expressed as the ratio of the weight of extract to the weight of dried seeds, the level of sulfhydryl compound was expressed as reduced L-glutathione (GSH) equivalent. The level of sulfhydryl compounds was determined by Ellman reagent and further analyzed using a spectrophotometer at 411 nm wavelength. Stink bean produced the highest yield of extract, while the highest level of sulfhydryl compounds was obtained from petai cina seed extract. The concentration of ethanol gave a significant difference to the yield of extract and the level of sulfhydryl compounds. The results showed that ethanol concentration affected the efficiency of the extraction of total sulfhydryl compounds and the yield of the extract on leguminous seeds.Sri WardatunYahdiana HarahapAbdul Mun'imNOORWATI SUTANDYOMBI & UNS Soloarticleleguminous seed, solvent, sulfhydryl compound, yield of extractBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	leguminous seed, solvent, sulfhydryl compound, yield of extract Biology (General) QH301-705.5
spellingShingle	leguminous seed, solvent, sulfhydryl compound, yield of extract Biology (General) QH301-705.5 Sri Wardatun Yahdiana Harahap Abdul Mun'im NOORWATI SUTANDYO Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations
description	Abstract. Wardatun S, Harahap Y, Mun’im A. 2020. Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations. Biodiversitas 21: 1060-1064. Leucaena leucocephala (Lam.) de Wit (petai Cina), Parkia speciosa Hassk. (petai), and Archidendron jiringa (Jack) I.C. Nielsen (jengkol) seeds extracts contain sulfhydryl compounds and have various therapeutic properties. The main objective of this research was to compare the effect of various ethanol concentration on the yield of extract and levels of sulfhydryl compounds from leguminous seeds. Dried seeds were macerated at room temperature (25°C) with a ratio of solids and solvents was 1:10. The solvent concentrations used were 30%, 50%, 70% and 96% ethanol. The yield of extract was expressed as the ratio of the weight of extract to the weight of dried seeds, the level of sulfhydryl compound was expressed as reduced L-glutathione (GSH) equivalent. The level of sulfhydryl compounds was determined by Ellman reagent and further analyzed using a spectrophotometer at 411 nm wavelength. Stink bean produced the highest yield of extract, while the highest level of sulfhydryl compounds was obtained from petai cina seed extract. The concentration of ethanol gave a significant difference to the yield of extract and the level of sulfhydryl compounds. The results showed that ethanol concentration affected the efficiency of the extraction of total sulfhydryl compounds and the yield of the extract on leguminous seeds.
format	article
author	Sri Wardatun Yahdiana Harahap Abdul Mun'im NOORWATI SUTANDYO
author_facet	Sri Wardatun Yahdiana Harahap Abdul Mun'im NOORWATI SUTANDYO
author_sort	Sri Wardatun
title	Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations
title_short	Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations
title_full	Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations
title_fullStr	Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations
title_full_unstemmed	Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations
title_sort	short communication: optimization of extraction of sulfhydryl compounds from several legumes seeds in indonesia with various ethanol concentrations
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/25d02cbddcc849568e661c8bee9cc812
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Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations

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