Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1

Abstract The Phospholipase A2 Receptor 1 (PLA2R1) was first identified for its ability to bind some secreted PLA2s (sPLA2s). It belongs to the C-type lectin superfamily and it binds different types of proteins. It is likely a multifunctional protein that plays a role i) in inflammation and inflammat...

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Autores principales:	Sara Jaber, Delphine Goehrig, Philippe Bertolino, Amélie Massemin, Franck Bihl, Joëlle Chabry, Gérard Lambeau, David Vindrieux, David Bernard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/25d2faad67014d5f9762d7d3ad7875d3
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spelling	oai:doaj.org-article:25d2faad67014d5f9762d7d3ad7875d32021-12-02T14:59:09ZGeneration of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 110.1038/s41598-020-64863-y2045-2322https://doaj.org/article/25d2faad67014d5f9762d7d3ad7875d32020-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-64863-yhttps://doaj.org/toc/2045-2322Abstract The Phospholipase A2 Receptor 1 (PLA2R1) was first identified for its ability to bind some secreted PLA2s (sPLA2s). It belongs to the C-type lectin superfamily and it binds different types of proteins. It is likely a multifunctional protein that plays a role i) in inflammation and inflammatory diseases, ii) in cellular senescence, a mechanism participating in aging and age-related diseases including cancer, and iii) in membranous nephropathy (MN), a rare autoimmune kidney disease where PLA2R1 is the major autoantigen. To help study the role of PLA2R1 in these pathophysiological conditions, we have generated a versatile NeoR-hPLA2R1 conditional transgenic mice which will allow the specific expression of human PLA2R1 (hPLA2R1) in relevant organs and cells following Cre recombinase-driven excision of the NeoR-stop cassette flanked by LoxP sites. Proof-of-concept breeding of NeoR-hPLA2R1 mice with the ubiquitous adenoviral EIIa promoter-driven Cre mouse line resulted in the expected excision of the NeoR-stop cassette and the expression of hPLA2R1 in all tested tissues. These Tg-hPLA2R1 animals breed normally, with no reproduction or apparent growth defect. These models, especially the NeoR-hPLA2R1 conditional transgenic mouse line, will facilitate the future investigation of PLA2R1 functions in relevant pathophysiological contexts, including inflammatory diseases, age-related diseases and MN.Sara JaberDelphine GoehrigPhilippe BertolinoAmélie MasseminFranck BihlJoëlle ChabryGérard LambeauDavid VindrieuxDavid BernardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Jaber Delphine Goehrig Philippe Bertolino Amélie Massemin Franck Bihl Joëlle Chabry Gérard Lambeau David Vindrieux David Bernard Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
description	Abstract The Phospholipase A2 Receptor 1 (PLA2R1) was first identified for its ability to bind some secreted PLA2s (sPLA2s). It belongs to the C-type lectin superfamily and it binds different types of proteins. It is likely a multifunctional protein that plays a role i) in inflammation and inflammatory diseases, ii) in cellular senescence, a mechanism participating in aging and age-related diseases including cancer, and iii) in membranous nephropathy (MN), a rare autoimmune kidney disease where PLA2R1 is the major autoantigen. To help study the role of PLA2R1 in these pathophysiological conditions, we have generated a versatile NeoR-hPLA2R1 conditional transgenic mice which will allow the specific expression of human PLA2R1 (hPLA2R1) in relevant organs and cells following Cre recombinase-driven excision of the NeoR-stop cassette flanked by LoxP sites. Proof-of-concept breeding of NeoR-hPLA2R1 mice with the ubiquitous adenoviral EIIa promoter-driven Cre mouse line resulted in the expected excision of the NeoR-stop cassette and the expression of hPLA2R1 in all tested tissues. These Tg-hPLA2R1 animals breed normally, with no reproduction or apparent growth defect. These models, especially the NeoR-hPLA2R1 conditional transgenic mouse line, will facilitate the future investigation of PLA2R1 functions in relevant pathophysiological contexts, including inflammatory diseases, age-related diseases and MN.
format	article
author	Sara Jaber Delphine Goehrig Philippe Bertolino Amélie Massemin Franck Bihl Joëlle Chabry Gérard Lambeau David Vindrieux David Bernard
author_facet	Sara Jaber Delphine Goehrig Philippe Bertolino Amélie Massemin Franck Bihl Joëlle Chabry Gérard Lambeau David Vindrieux David Bernard
author_sort	Sara Jaber
title	Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
title_short	Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
title_full	Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
title_fullStr	Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
title_full_unstemmed	Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
title_sort	generation of a conditional transgenic mouse model expressing human phospholipase a2 receptor 1
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/25d2faad67014d5f9762d7d3ad7875d3
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Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1

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