Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri

The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of the Huanglongbing (HLB), or citrus greening disease. This disease has devastated Florida’s citrus industry, and threatens California’s industry as well as other citru...

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Autores principales:	Chad Vosburg, Max Reynolds, Rita Noel, Teresa Shippy, Prashant S. Hosmani, Mirella Flores-Gonzalez, Lukas A. Mueller, Wayne B. Hunter, Susan J. Brown, Tom D’Elia, Surya Saha
Formato:	article
Lenguaje:	EN
Publicado:	GigaScience Press 2021
Materias:	Electronic computers. Computer science QA75.5-76.95
Acceso en línea:	https://doaj.org/article/25f8bc1b9d1046a8a412373943b1b23a
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spelling	oai:doaj.org-article:25f8bc1b9d1046a8a412373943b1b23a2021-12-02T17:21:21ZUtilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri2709-47152709-471510.46471/gigabyte.21https://doaj.org/article/25f8bc1b9d1046a8a412373943b1b23a2021-05-01T00:00:00Zhttps://gigabytejournal.com/articles/21https://doaj.org/toc/2709-4715https://doaj.org/toc/2709-4715The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of the Huanglongbing (HLB), or citrus greening disease. This disease has devastated Florida’s citrus industry, and threatens California’s industry as well as other citrus producing regions around the world. To find novel solutions to the disease, a better understanding of the vector is needed. The D. citri genome has been used to identify and characterize genes involved in Wnt signaling pathways. Wnt signaling is utilized for many important biological processes in metazoans, such as patterning and tissue generation. Curation based on RNA sequencing data and sequence homology confirms 24 Wnt signaling genes within the D. citri genome, including homologs for beta-catenin, Frizzled receptors, and seven Wnt-ligands. Through phylogenetic analysis, we classify D. citri Wnt ligands as Wg/Wnt1, Wnt5, Wnt6, Wnt7, Wnt10, Wnt11, and WntA. The D. citri version 3.0 genome with chromosomal length scaffolds reveals a conserved Wnt1-Wnt6-Wnt10 gene cluster with a gene configuration like that in Drosophila melanogaster. These findings provide greater insight into the evolutionary history of D. citri and Wnt signaling in this important hemipteran vector. Manual annotation was essential for identifying high quality gene models. These gene models can be used to develop molecular systems, such as CRISPR and RNAi, which target and control psyllid populations to manage the spread of HLB. Manual annotation of Wnt signaling pathways was done as part of a collaborative community annotation project.Chad VosburgMax ReynoldsRita NoelTeresa ShippyPrashant S. HosmaniMirella Flores-GonzalezLukas A. MuellerWayne B. HunterSusan J. BrownTom D’EliaSurya SahaGigaScience PressarticleElectronic computers. Computer scienceQA75.5-76.95ENGigaByte, Vol 2021, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electronic computers. Computer science QA75.5-76.95
spellingShingle	Electronic computers. Computer science QA75.5-76.95 Chad Vosburg Max Reynolds Rita Noel Teresa Shippy Prashant S. Hosmani Mirella Flores-Gonzalez Lukas A. Mueller Wayne B. Hunter Susan J. Brown Tom D’Elia Surya Saha Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri
description	The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of the Huanglongbing (HLB), or citrus greening disease. This disease has devastated Florida’s citrus industry, and threatens California’s industry as well as other citrus producing regions around the world. To find novel solutions to the disease, a better understanding of the vector is needed. The D. citri genome has been used to identify and characterize genes involved in Wnt signaling pathways. Wnt signaling is utilized for many important biological processes in metazoans, such as patterning and tissue generation. Curation based on RNA sequencing data and sequence homology confirms 24 Wnt signaling genes within the D. citri genome, including homologs for beta-catenin, Frizzled receptors, and seven Wnt-ligands. Through phylogenetic analysis, we classify D. citri Wnt ligands as Wg/Wnt1, Wnt5, Wnt6, Wnt7, Wnt10, Wnt11, and WntA. The D. citri version 3.0 genome with chromosomal length scaffolds reveals a conserved Wnt1-Wnt6-Wnt10 gene cluster with a gene configuration like that in Drosophila melanogaster. These findings provide greater insight into the evolutionary history of D. citri and Wnt signaling in this important hemipteran vector. Manual annotation was essential for identifying high quality gene models. These gene models can be used to develop molecular systems, such as CRISPR and RNAi, which target and control psyllid populations to manage the spread of HLB. Manual annotation of Wnt signaling pathways was done as part of a collaborative community annotation project.
format	article
author	Chad Vosburg Max Reynolds Rita Noel Teresa Shippy Prashant S. Hosmani Mirella Flores-Gonzalez Lukas A. Mueller Wayne B. Hunter Susan J. Brown Tom D’Elia Surya Saha
author_facet	Chad Vosburg Max Reynolds Rita Noel Teresa Shippy Prashant S. Hosmani Mirella Flores-Gonzalez Lukas A. Mueller Wayne B. Hunter Susan J. Brown Tom D’Elia Surya Saha
author_sort	Chad Vosburg
title	Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri
title_short	Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri
title_full	Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri
title_fullStr	Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri
title_full_unstemmed	Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri
title_sort	utilizing a chromosomal-length genome assembly to annotate the wnt signaling pathway in the asian citrus psyllid, diaphorina citri
publisher	GigaScience Press
publishDate	2021
url	https://doaj.org/article/25f8bc1b9d1046a8a412373943b1b23a
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Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri

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