The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content>
ABSTRACT Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophytho...
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American Society for Microbiology
2020
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oai:doaj.org-article:25fa72ccc0484e6eae9ac0250877956f2021-11-15T15:30:15ZThe Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content>10.1128/mSphere.00261-202379-5042https://doaj.org/article/25fa72ccc0484e6eae9ac0250877956f2020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00261-20https://doaj.org/toc/2379-5042ABSTRACT Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophythora litchii, named PlBZP32. We found that PlBZP32 is upregulated in zoospores, in cysts, and during invasive hyphal growth. We studied the functions of PlBZP32 using the RNAi technique to suppress the expression of this gene. PlBZP32-silenced mutants were more sensitive to oxidative stress, showed a lower cyst germination rate, and produced more sporangia than the wild-type strain SHS3. The PlBZP32-silenced mutants were also less invasive on the host plant. Furthermore, we analyzed the activities of extracellular peroxidases and laccases and found that silencing PlBZP32 decreased the activities of P. litchii peroxidase and laccase. To our knowledge, this is the first report that the functions of a bZIP-PAS protein are associated with oxidative stress, asexual development, and pathogenicity in oomycetes. IMPORTANCE In this study, we utilized the RNAi technique to investigate the functions of PlBZP32, which possesses a basic leucine zipper (bZIP)-PAS structure, and provided insights into the contributions of bZIP transcription factors to oxidative stress, the production of sporangia, the germination of cysts, and the pathogenicity of Peronophythora litchii. This study also revealed the role of PlBZP32 in regulating the enzymatic activities of extracellular peroxidases and laccases in the plant-pathogenic oomycete.Guanghui KongYubin ChenYizhen DengDinan FengLiqun JiangLang WanMinhui LiZide JiangPinggen XiAmerican Society for MicrobiologyarticlePeronophythora litchiibZIP transcription factorpathogenicityperoxidaselaccaseMicrobiologyQR1-502ENmSphere, Vol 5, Iss 3 (2020) |
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Peronophythora litchii bZIP transcription factor pathogenicity peroxidase laccase Microbiology QR1-502 |
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Peronophythora litchii bZIP transcription factor pathogenicity peroxidase laccase Microbiology QR1-502 Guanghui Kong Yubin Chen Yizhen Deng Dinan Feng Liqun Jiang Lang Wan Minhui Li Zide Jiang Pinggen Xi The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| description |
ABSTRACT Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophythora litchii, named PlBZP32. We found that PlBZP32 is upregulated in zoospores, in cysts, and during invasive hyphal growth. We studied the functions of PlBZP32 using the RNAi technique to suppress the expression of this gene. PlBZP32-silenced mutants were more sensitive to oxidative stress, showed a lower cyst germination rate, and produced more sporangia than the wild-type strain SHS3. The PlBZP32-silenced mutants were also less invasive on the host plant. Furthermore, we analyzed the activities of extracellular peroxidases and laccases and found that silencing PlBZP32 decreased the activities of P. litchii peroxidase and laccase. To our knowledge, this is the first report that the functions of a bZIP-PAS protein are associated with oxidative stress, asexual development, and pathogenicity in oomycetes. IMPORTANCE In this study, we utilized the RNAi technique to investigate the functions of PlBZP32, which possesses a basic leucine zipper (bZIP)-PAS structure, and provided insights into the contributions of bZIP transcription factors to oxidative stress, the production of sporangia, the germination of cysts, and the pathogenicity of Peronophythora litchii. This study also revealed the role of PlBZP32 in regulating the enzymatic activities of extracellular peroxidases and laccases in the plant-pathogenic oomycete. |
| format |
article |
| author |
Guanghui Kong Yubin Chen Yizhen Deng Dinan Feng Liqun Jiang Lang Wan Minhui Li Zide Jiang Pinggen Xi |
| author_facet |
Guanghui Kong Yubin Chen Yizhen Deng Dinan Feng Liqun Jiang Lang Wan Minhui Li Zide Jiang Pinggen Xi |
| author_sort |
Guanghui Kong |
| title |
The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_short |
The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_full |
The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_fullStr |
The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_full_unstemmed |
The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_sort |
basic leucine zipper transcription factor plbzp32 associated with the oxidative stress response is critical for pathogenicity of the lychee downy blight oomycete <named-content content-type="genus-species">peronophythora litchii</named-content> |
| publisher |
American Society for Microbiology |
| publishDate |
2020 |
| url |
https://doaj.org/article/25fa72ccc0484e6eae9ac0250877956f |
| work_keys_str_mv |
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